Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	990,590	C→G	6.6%	R157R (CGC→CGG) ‡	PP_0856 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	990,590	0	C	G	6.6%	242.3 / 7.0	76	R157R (CGC→CGG) ‡	PP_0856	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (36/35);  new base G (2/3);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

GTCATGCTCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTGG  >  NC_002947/990444‑990738                                                                                                                                                   |                                                                                                                                                     gTCATGCTCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGc                                                                                                                                                      <  8:29239/147‑1 (MQ=255) gTCATGCTCGGTACCCTCAAGGGTGAAGGCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCt                                                                                                                                                    <  5:169292/149‑1 (MQ=255)      gCTCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCg                                                                                                                                               >  8:216231/1‑149 (MQ=255)        tCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGc                                                                                                                                             >  6:263715/1‑149 (MQ=255)          ggTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCt                                                                                                                                           <  6:386024/149‑1 (MQ=255)            tACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         <  3:316889/149‑1 (MQ=255)                   aaGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATgccgc                                                                                                                                  >  4:297519/1‑149 (MQ=255)                        tGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         <  1:266708/137‑1 (MQ=255)                        tGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         >  2:266708/1‑137 (MQ=255)                          aaGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGc                                                                                                                           <  7:216231/149‑1 (MQ=255)                             tCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGAc                                                                                                                        <  2:9025/149‑1 (MQ=255)                               aTTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         <  4:389131/130‑1 (MQ=255)                               aTTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         >  3:389131/1‑130 (MQ=255)                               aTTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGAccg                                                                                                                      >  7:153074/1‑149 (MQ=255)                                   cccTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGCCCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGt                                                                                                                  >  7:355600/1‑149 (MQ=255)                                        gACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTgg                                                                                                               <  3:297519/147‑1 (MQ=255)                                             cAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTAc                                                                                                         >  5:415219/1‑148 (MQ=255)                                              aGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGAt                                                                                                                                       >  5:135024/1‑117 (MQ=255)                                              aGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGAt                                                                                                                                       <  6:135024/117‑1 (MQ=255)                                              aGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACg                                                                                                        >  2:299564/1‑148 (MQ=255)                                                   cGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACt                                                                                                  >  3:159227/1‑149 (MQ=255)                                                   cGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACt                                                                                                  >  5:7627/1‑149 (MQ=255)                                                    ggTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTc                                                                                                 <  7:91317/149‑1 (MQ=255)                                                     gTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCg                                                                                                >  7:390538/1‑149 (MQ=255)                                                          gagCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGAGCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggc                                                                                           >  3:138966/1‑149 (MQ=255)                                                          gagCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggc                                                                                           >  3:13403/1‑149 (MQ=255)                                                          gagCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggc                                                                                           <  4:159227/149‑1 (MQ=255)                                                           agCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggcc                                                                                          <  4:69074/149‑1 (MQ=255)                                                           agCGGTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggcc                                                                                          <  4:13403/149‑1 (MQ=255)                                                                 ggCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCt                                                                                     <  7:33694/148‑1 (MQ=255)                                                                  gcgcTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGa                                                                                   >  3:27167/1‑149 (MQ=255)                                                                      tCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCt                                                                               >  2:231427/1‑149 (MQ=255)                                                                        gcgcgTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGc                                                                             <  6:415219/149‑1 (MQ=255)                                                                        gcgcgTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGc                                                                             >  7:146122/1‑149 (MQ=255)                                                                          gtgTGACCATCGAAGTGCTGGCCCCTCCTGCCAACAACGGTGAGGTGGTGGTGGTGCAGAGCCAGGACTATCGCCTGATGGGCGTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGt                                                                           <  4:138966/147‑1 (MQ=255)                                                                           cgTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTg                                                                          <  1:299564/149‑1 (MQ=255)                                                                            gTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTgg                                                                         <  2:132115/149‑1 (MQ=255)                                                                                ccAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGGTGGATCTAc                                                                                                         >  7:339888/1‑113 (MQ=255)                                                                                 cAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCg                                                                    >  8:168086/1‑149 (MQ=255)                                                                                  aGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgc                                                                                               >  8:120180/1‑121 (MQ=255)                                                                                  aGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgc                                                                                               <  7:120180/121‑1 (MQ=255)                                                                                      aaGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGtt                                                                                                                 <  5:411597/99‑1 (MQ=255)                                                                                      aaGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGtt                                                                                                                 >  6:411597/1‑99 (MQ=255)                                                                                         tGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGcc                                                             >  3:157180/1‑148 (MQ=255)                                                                                              gCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATc                                                         <  7:168086/147‑1 (MQ=255)                                                                                               ccccACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGc                                                       >  4:401702/1‑148 (MQ=255)                                                                                                cccACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGcca                                                     <  5:263715/149‑1 (MQ=255)                                                                                                         caacaaCGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGcc                                            >  3:355264/1‑149 (MQ=255)                                                                                                         caacaaCGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGcc                                            >  6:358653/1‑149 (MQ=255)                                                                                                          aacaacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGTTGCCGCCTCTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCg                                                                    <  7:138443/124‑1 (MQ=255)                                                                                                          aacaacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGTTGCCGCCTCTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCg                                                                    >  8:138443/1‑124 (MQ=255)                                                                                                            caacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCTCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTgg                                         <  8:153074/149‑1 (MQ=255)                                                                                                             aacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGc                                        >  3:373922/1‑149 (MQ=255)                                                                                                                    aCGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGc                                  <  8:284226/148‑1 (MQ=255)                                                                                                                    aCGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGc                                  >  7:284226/1‑148 (MQ=255)                                                                                                                     cGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCg                                <  8:146122/149‑1 (MQ=255)                                                                                                                      gtggtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTg                                          <  4:243116/138‑1 (MQ=255)                                                                                                                      gtggtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTg                                          >  3:243116/1‑138 (MQ=255)                                                                                                                         gtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCt                            <  4:27167/149‑1 (MQ=255)                                                                                                                            gtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCa                                                                       <  7:125112/103‑1 (MQ=255)                                                                                                                            gtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCa                                                                       >  8:125112/1‑103 (MQ=255)                                                                                                                               gtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTAc                      <  4:373922/149‑1 (MQ=255)                                                                                                                                     aCCCAGGCCGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAATCTCCCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCaa                <  8:355600/149‑1 (MQ=255)                                                                                                                                       ccAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCt                            <  4:336124/135‑1 (MQ=255)                                                                                                                                       ccAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCt                            >  3:336124/1‑135 (MQ=255)                                                                                                                                         aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGGTCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAgg              >  4:373196/1‑147 (MQ=255)                                                                                                                                         aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAgg              >  4:378171/1‑147 (MQ=255)                                                                                                                                         aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCAGGCGGTTggtg                                <  3:76799/129‑4 (MQ=255)                                                                                                                                         aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCAGGCGGTTggtg                                >  4:76799/1‑126 (MQ=255)                                                                                                                                           gACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGt          >  8:46203/1‑149 (MQ=255)                                                                                                                                              gATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGc       >  3:388125/1‑149 (MQ=255)                                                                                                                                              gATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGc       >  8:284371/1‑149 (MQ=255)                                                                                                                                               aTCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCg      <  4:355264/149‑1 (MQ=255)                                                                                                                                                tCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCgg     <  3:401702/149‑1 (MQ=255)                                                                                                                                                  gCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGt    <  7:284371/148‑1 (MQ=255)                                                                                                                                                  gCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTg   <  6:7627/149‑1 (MQ=255)                                                                                                                                                  gCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTg   <  4:157180/149‑1 (MQ=255)                                                                                                                                                   ccTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTgg  >  3:342921/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GTCATGCTCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTGG  >  NC_002947/990444‑990738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑