Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	990,969	T→G	5.6%	Y284D (TAC→GAC)	PP_0856 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	990,969	0	T	G	5.6%	208.1 / 2.1	67	Y284D (TAC→GAC)	PP_0856	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (36/27);  new base G (2/2);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGGCTTGTTGCTGTCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACTTTGAAG  >  NC_002947/990824‑991110                                                                                                                                                  |                                                                                                                                              cggcggCTTGTTGCTGTCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCt                                                                                                                                               <  6:52623/146‑1 (MQ=255) cggcggCTTGTTGCTGTCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCt                                                                                                                                               >  5:52623/1‑146 (MQ=255)          gttgCTGTCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAg                                                                                                                                   >  3:161789/1‑149 (MQ=255)           ttgCTGTCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGc                                                                                                                                  <  5:307467/149‑1 (MQ=255)            tgCTGTCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGcc                                                                                                                                 >  6:356099/1‑149 (MQ=255)             gCTGTCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGTTAACGTCTACGTCATCGAAGCCt                                                                                                                                >  6:102705/1‑149 (MQ=255)                 tCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGCTGACGTAGACGTTACCGAAGccctggg                                                                                                                            <  6:824/149‑6 (MQ=38)                 tCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGCTGACGTAGACGTTACCGAAGccctgg                                                                                                                             <  4:52637/148‑5 (MQ=255)                   tggtggTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCGACGTCAGCGAAGCCTCGGGTa                                                                                                                          >  4:163757/1‑149 (MQ=255)                      ggttctccTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCg                                                                                                                       <  8:6081/143‑1 (MQ=255)                       ggtACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGt                                                                                                                      >  1:215888/1‑149 (MQ=255)                           cccTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCACGGGTACGGTGGa                                                                                                                   >  1:347474/1‑148 (MQ=255)                           cccTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGGGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGa                                                                                                                   >  8:90088/1‑148 (MQ=255)                             cTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCtga                                                                                                                              <  4:65255/135‑3 (MQ=255)                             cTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCtga                                                                                                                              >  3:65255/1‑133 (MQ=255)                               gTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAgtg                                                                                                              >  4:119657/1‑149 (MQ=255)                                      aGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGa                                                                                                       <  5:300047/149‑1 (MQ=255)                                        cACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAgc                                                                                                     >  2:376958/1‑149 (MQ=255)                                                aGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTc                                                                                              >  6:180391/1‑148 (MQ=255)                                                   gCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGGGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGt                                                                                          <  7:90088/149‑1 (MQ=255)                                                      gtgtAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGcc                                                                                       <  4:161789/149‑1 (MQ=255)                                                       tgtAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGAt                                                                                                         >  1:197865/1‑130 (MQ=255)                                                       tgtAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGAt                                                                                                         <  2:197865/130‑1 (MQ=255)                                                             gggCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGa                                                                                <  3:221206/149‑1 (MQ=255)                                                                    gacctggaAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGa                                                                         >  4:222310/1‑149 (MQ=255)                                                                      cctggaAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACa                                                                       <  1:205469/149‑1 (MQ=255)                                                                         ggaAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTgg                                                                                                                    >  5:269946/1‑101 (MQ=255)                                                                         ggaAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTgg                                                                                                                    <  6:269946/101‑1 (MQ=255)                                                                          gaAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCAt                                                                   >  4:331417/1‑149 (MQ=255)                                                                           aaaGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATg                                                                  <  8:8905/149‑1 (MQ=255)                                                                            aaGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGc                                                                              >  3:368016/1‑136 (MQ=255)                                                                            aaGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTGCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGc                                                                              <  4:368016/136‑1 (MQ=255)                                                                                  ccGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTcgcc                                                           >  5:213787/1‑149 (MQ=255)                                                                                   cgcgAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGa                                                                         >  4:191315/1‑134 (MQ=255)                                                                                   cgcgAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGa                                                                         <  3:191315/134‑1 (MQ=255)                                                                                      gAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCa                                                       <  1:88617/149‑1 (MQ=255)                                                                                      gAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCa                                                       <  3:119657/149‑1 (MQ=255)                                                                                        gCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGt                                                     <  2:215888/149‑1 (MQ=255)                                                                                         cTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAggt                                                    <  1:376958/149‑3 (MQ=255)                                                                                            tgGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGTAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGAc                                                 >  8:80604/1‑149 (MQ=255)                                                                                            tgGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGAc                                                 <  6:20551/149‑1 (MQ=255)                                                                                            tgGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGAc                                                 >  5:20551/1‑149 (MQ=255)                                                                                              gCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGaaa                                                                                                                 <  1:46974/83‑1 (MQ=255)                                                                                              gCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGaaa                                                                                                                 >  2:46974/1‑83 (MQ=255)                                                                                               cAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGc                                              >  7:101491/1‑149 (MQ=255)                                                                                                aGCGCGATGCTTACAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGca                                             >  4:263522/1‑148 (MQ=255)                                                                                                      aTGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAAc                                                                           >  5:372301/1‑113 (MQ=255)                                                                                                      aTGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAAc                                                                           <  6:372301/113‑1 (MQ=255)                                                                                                              aGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTcag                               >  7:166177/1‑149 (MQ=255)                                                                                                               gCTATGTGGGTGTCGCTCAGGGCTTCTGTAACGTCTACGTCATCGAAGCCTCGTGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTcagc                              <  5:102705/149‑1 (MQ=255)                                                                                                                cTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCa                                                       <  2:139239/123‑1 (MQ=255)                                                                                                                cTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCa                                                       >  1:139239/1‑123 (MQ=255)                                                                                                                     tgGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTAc                        >  6:185457/1‑149 (MQ=255)                                                                                                                        gtgtCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACAGCCCCTGAAGTGTACAGCAGCTACGTg                     >  8:69470/1‑149 (MQ=255)                                                                                                                          gtCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGc                   >  2:361206/1‑149 (MQ=255)                                                                                                                             gCCCAGGGCTTCGGTAACGTCTACGTCAGCGTAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCtgt                <  7:80604/149‑1 (MQ=255)                                                                                                                             gCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCtgt                <  3:222310/149‑1 (MQ=255)                                                                                                                              cccAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCtgtg               >  5:271583/1‑149 (MQ=255)                                                                                                                               ccAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTgg              >  4:60295/1‑149 (MQ=255)                                                                                                                                cgaggCTTCGCTGACGTAGACGTTACCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTggg             >  1:331600/4‑149 (MQ=255)                                                                                                                                     cTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACt        >  2:310549/1‑149 (MQ=255)                                                                                                                                     cTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACt        >  2:315865/1‑149 (MQ=255)                                                                                                                                        cGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTg                                         >  4:281004/1‑113 (MQ=255)                                                                                                                                        cGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTg                                         <  3:281004/113‑1 (MQ=255)                                                                                                                                          gTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACTTTg     >  1:297187/1‑147 (MQ=255)                                                                                                                                          gTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCACCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACTTTg     >  5:274511/1‑147 (MQ=255)                                                                                                                                          gTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCACCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACTTTGaa   <  6:274511/149‑1 (MQ=255)                                                                                                                                                 cTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACTTTGAAg  <  3:10837/143‑1 (MQ=255)                                                                                                                                                 cTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACTTTGAAg  >  4:10837/1‑143 (MQ=255)                                                                                                                                                  |                                                                                                                                              CGGCGGCTTGTTGCTGTCTGGTGGTACCCTGTACGTCAGCACCTACCAGGGCCGTGTAGCGGGCCTGGACCTGGAAAGCGGCCGCGAGCTGTGGCAGCGCGATGCTTCCAGCTATGTGGGTGTCGCCCAGGGCTTCGGTAACGTCTACGTCAGCGAAGCCTCGGGTACCGTGGAAAGTGTGGATGAGCGCTCTTCCAGTGCCCTGTGGAGCAACGACAGCATGGCTCGCCGCCAGTTGACCGCCCCTGAAGTGTTCAGCAGCTACGTGGCTGTGGGTGACTTTGAAG  >  NC_002947/990824‑991110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑