Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	991,333	C→A	6.1%	intergenic (+71/‑46)	PP_0856 → / → der	lipoprotein/GTPase Der

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	991,333	0	C	A	6.1%	211.4 / 3.2	66	intergenic (+71/‑46)	PP_0856/der	lipoprotein/GTPase Der
Reads supporting (aligned to +/- strand):  ref base C (30/32);  new base A (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

CCGCCCACTTGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGG  >  NC_002947/991187‑991481                                                                                                                                                   |                                                                                                                                                     ccgcccACTTGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCtt                                                                                                                                                    >  6:112880/1‑149 (MQ=255) ccgcccACTTGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCtt                                                                                                                                                    <  3:135898/149‑1 (MQ=255)  cgcccACTTGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTg                                                                                                                                                   >  2:11231/1‑149 (MQ=255)   gcccACTTGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTg                                                                                                                                                   >  6:199848/1‑148 (MQ=255)     ccACTTGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAg                                                                                                                                                >  8:100449/1‑149 (MQ=255)      cACTTGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGc                                                                                                                                               <  5:195722/149‑1 (MQ=255)         ttGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGc                                                                                                                                            >  8:33990/1‑149 (MQ=255)              gTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCGTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCAGGCCGCTGCAAGGCAGCGGccgga                                                                                                                                        <  4:55035/148‑4 (MQ=255)              gTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCttt                                                                                                                                        <  3:274706/148‑1 (MQ=255)              gTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCttt                                                                                                                                        <  6:204752/148‑1 (MQ=255)              gTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTg                                                                                                                                       >  5:195176/1‑149 (MQ=255)                   ccgatctaTCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCAtt                                                                                                                                   <  1:174668/141‑1 (MQ=255)                       acCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCaa                                                                                                                              <  2:368287/149‑1 (MQ=255)                           tCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCAtt                                                                                                                                   >  2:174668/1‑140 (MQ=255)                              aTGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGc                                                                                                                                            >  3:4619/1‑128 (MQ=255)                              aTGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGc                                                                                                                                            <  4:4619/128‑1 (MQ=255)                              aTGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCAt                                                                                                                                    >  6:334980/1‑136 (MQ=255)                               tGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCga                                                                                                                      <  7:100449/149‑1 (MQ=255)                                 tCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCgag                                                                                                                     <  5:199848/148‑1 (MQ=255)                                     cGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCTCCGGCCTTTGCATTTTCAAGAATTCGAGAGt                                                                                                                  <  1:36838/147‑1 (MQ=255)                                         aaCAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGCCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGACAGTg                                                                                                                 >  4:270118/1‑144 (MQ=255)                                           cAGCGGCAAGCTCGAGGCACTGACCATCCGCACAAGCTAAGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGAAGGACACGCCGCCCAAACACCGCCCGCTGCCTAGCACCGGCCtat                                                                                                                                        >  3:371797/1‑117 (MQ=255)                                              cGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGAt                                                                                                       <  4:19572/149‑1 (MQ=255)                                              cGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGAt                                                                                                       >  4:367456/1‑149 (MQ=255)                                               ggCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATg                                                                                                      <  3:7413/149‑1 (MQ=255)                                               ggCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATg                                                                                                      >  6:377540/1‑149 (MQ=255)                                                   aGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGTCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGGATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTc                                                                                                  <  3:270118/149‑1 (MQ=255)                                                   aGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTc                                                                                                  <  6:195176/149‑1 (MQ=255)                                                      tCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGc                                                                                                                                               >  3:387525/1‑101 (MQ=255)                                                      tCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGc                                                                                                                                               <  4:387525/101‑1 (MQ=255)                                                        gAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGc                                                                                                                                            >  7:129182/1‑102 (MQ=255)                                                        gAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGc                                                                                                                                            <  8:129182/102‑1 (MQ=255)                                                            cACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCga                                                                                                                      >  7:91669/1‑120 (MQ=255)                                                            cACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCga                                                                                                                      <  8:91669/120‑1 (MQ=255)                                                             aCTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGc                                                                                        >  3:183552/1‑149 (MQ=255)                                                                   cATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCtggt                                                                                  <  7:33990/149‑1 (MQ=255)                                                                    aTCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCCAACTCCGGCAGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCtggtg                                                                                 >  7:266846/1‑149 (MQ=255)                                                                    aTCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCtggtg                                                                                 >  8:229675/1‑149 (MQ=255)                                                                     tCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTAGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCtggtgg                                                                                <  4:294021/149‑1 (MQ=255)                                                                           gAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTGCCGTAATTGCCCTGGTGGgccgcc                                                                          >  1:193434/1‑149 (MQ=255)                                                                                tATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAAc                                                                     >  5:366410/1‑149 (MQ=255)                                                                                   gCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGGGGCCTTTGCATTTTCAAGAATTCGAGAGTGGTGATCCGCTGGTTGCCGTAATTGCCCTGGTGGGCCGCCCGCACg                                                                    >  7:139143/1‑147 (MQ=255)                                                                                        aaGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTGGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCa                                                              >  4:267612/1‑148 (MQ=255)                                                                                         aGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCaaa                                                            <  4:183552/149‑1 (MQ=255)                                                                                                      ttCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTc                                                >  6:324664/1‑148 (MQ=255)                                                                                                       tCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGt                                                                                              >  7:172439/1‑101 (MQ=255)                                                                                                       tCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGt                                                                                              <  8:172439/101‑1 (MQ=255)                                                                                                        cAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTACCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAAc                                             >  4:143415/1‑149 (MQ=255)                                                                                                         aaGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAAcc                                            <  8:280461/149‑1 (MQ=255)                                                                                                           gCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGc                                          <  3:267612/149‑1 (MQ=255)                                                                                                               cggcgTAGGACACGCCGCCCAATCTCCGGCCGGTGCCTTGCAGCGGCCTGTGCATTTTCAAGAATTCGAGAGTGGAGAGCGGATGGTTCCCGTAATTGCGCTGGTGGGCCGCCCGAAGGTCTGCAAATCCACCATGTTCATCCGCCTGa                                      <  6:324418/149‑1 (MQ=255)                                                                                                                  cgTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCa                                   <  3:290483/149‑1 (MQ=255)                                                                                                                   gTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCaa                                  <  5:324664/149‑1 (MQ=255)                                                                                                                      ggACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGa                                <  1:213168/148‑1 (MQ=255)                                                                                                                               gccAAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGc                      <  3:9950/149‑1 (MQ=255)                                                                                                                                 ccAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGTGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGAt                        >  8:270633/1‑145 (MQ=255)                                                                                                                                 ccAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGTGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGAt                        <  7:270633/145‑1 (MQ=255)                                                                                                                                  cAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGAt                        <  1:169545/144‑1 (MQ=255)                                                                                                                                  cAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTAAAGAATTCGAGAGTGGAGAGCCGATGGTTACCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAAAACACCAAGTTCAACCGCCTGACCAAGACCCCCCATgaga                    >  2:169545/1‑145 (MQ=255)                                                                                                                                          ggCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCTAACGTCGGCAAATCCACCATGTTCAACCGCCTTACCAAGACCCGCGATGCCATCGTCGGTg           <  1:11231/149‑1 (MQ=255)                                                                                                                                           gCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGa          <  3:143415/149‑1 (MQ=255)                                                                                                                                           gCCGCTGCAAGGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCAtcgt                <  7:210182/143‑1 (MQ=255)                                                                                                                                           gCCGCTGCAAGGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCAtcgt                >  8:210182/1‑143 (MQ=255)                                                                                                                                            ccGCTGCAAGGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGAc         >  2:122800/1‑149 (MQ=255)                                                                                                                                              gCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCt       >  3:223224/1‑149 (MQ=255)                                                                                                                                                 gCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTc    >  2:147104/1‑149 (MQ=255)                                                                                                                                                  ccTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCg   >  1:22971/1‑149 (MQ=255)                                                                                                                                                   cTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCgg  <  2:22971/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CCGCCCACTTGTGGTCGGCGACACCATCTATGTCTTCGGCAACAGCGGCAAGCTCGAGGCACTGACCATCCGCTGAAGCTATGCTTGAAGCCTTGGCGGGCTTCAAGCCGCGGCGTAGGACACGCCGCCCAAACTCCGGCCGCTGCCTTGCAGCGGCCTTTGCATTTTCAAGAATTCGAGAGTGGAGAGCCGATGGTTCCCGTAATTGCCCTGGTGGGCCGCCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGG  >  NC_002947/991187‑991481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑