Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,054,462	A→C	7.6%	M1G (GTG→GGG) †‡	PP_0912 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,054,462	0	A	C	7.6%	273.7 / 13.9	93	M1G (GTG→GGG) †‡	PP_0912	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (43/43);  new base C (5/2);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

TGGACGATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCA  >  NC_002947/1054315‑1054609                                                                                                                                                     |                                                                                                                                                    tGGACGATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGca                                                                                                                                                     >  5:317430/1‑148 (MQ=255) tGGACGATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGAACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGca                                                                                                                                                     >  7:218121/1‑148 (MQ=255)   gACGATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGcac                                                                                                                                                    >  8:320135/1‑147 (MQ=255)        ttCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCg                                                                                                                                             >  7:328299/1‑149 (MQ=255)        ttCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCg                                                                                                                                             <  7:74890/149‑1 (MQ=255)          cGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCgg                                                                                                                                            <  1:339884/148‑1 (MQ=255)          cGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCgg                                                                                                                                            <  3:32513/148‑1 (MQ=255)           ggCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCggg                                                                                                                                           <  5:187074/148‑1 (MQ=255)              cTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCGACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCAGGAACAAGGCCGGGCGtt                                                                                                                                       >  5:369332/1‑149 (MQ=255)               ttCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGttt                                                                                                                                      >  2:135827/1‑149 (MQ=255)                   ccccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑TAAAACGCCCGGCACAAGGCCGGGCGTTTGCgg                                                                                                                                  <  8:232496/149‑1 (MQ=255)                    cccAGACATCAAGAAGCTGCTGCACTGTGCCTCATGTTAGGTTTGATCGAAGGCATACAGTATGCGCATCTGCGTGCACGCCCGGCTGCGGGAAGCGTTTTACCCGGCTGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGt                                                                                                                                 <  6:276629/149‑1 (MQ=255)                     ccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTTCgttt                                                                                                                                <  7:320135/149‑4 (MQ=255)                     ccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTg                                                                                                                                <  3:337458/149‑1 (MQ=255)                      cAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTg                                                                                                                                >  6:91761/1‑148 (MQ=255)                       aGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGaa                                                                                                                              <  5:409664/149‑1 (MQ=255)                         aCATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAgc                                                                                                                            <  4:237525/149‑1 (MQ=255)                            tCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTg                                                                                                                                     >  8:111878/1‑137 (MQ=255)                            tCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTg                                                                                                                                     <  7:111878/137‑1 (MQ=255)                              aaGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTa                                                                                                                        >  8:346648/1‑148 (MQ=255)                              aaGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTa                                                                                                                        >  6:287552/1‑148 (MQ=255)                                      gcagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgtt                                                                                                                                 <  4:74662/131‑3 (MQ=38)                                      gcagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgtt                                                                                                                                 >  3:74662/1‑129 (MQ=38)                                          caCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGTGCTACAGCAGGATCAg                                                                                                            >  4:347712/1‑148 (MQ=255)                                          caCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAg                                                                                                            >  1:88456/1‑148 (MQ=255)                                                 cctcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCg                                                                                                                                   <  3:163610/118‑1 (MQ=255)                                                 cctcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCg                                                                                                                                   >  4:163610/1‑118 (MQ=255)                                                  ctcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCa                                                                                                             <  2:58701/139‑1 (MQ=255)                                                  ctcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCa                                                                                                             >  1:58701/1‑139 (MQ=255)                                                   tcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGt                                                                                                                                 >  6:49845/1‑118 (MQ=255)                                                   tcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGt                                                                                                                                 <  5:49845/118‑1 (MQ=255)                                                    cctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATc                                                                                                 >  2:291238/1‑149 (MQ=255)                                                     ctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGAt                                                                                                  <  8:338248/147‑1 (MQ=255)                                                                 aTCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGTGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCgg                                                                                     <  3:347712/148‑1 (MQ=255)                                                                 aTCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCgg                                                                                     <  8:328299/148‑1 (MQ=255)                                                                  tCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGt                                                                                   >  4:52004/1‑149 (MQ=255)                                                                    gAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGtt                                                                                  >  6:145234/1‑148 (MQ=255)                                                                    gAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACCCGTGATCCGCAGTGTCCGGGTTc                                                                                 <  2:88456/149‑1 (MQ=255)                                                                         ccTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGtt                                                                            <  3:52004/149‑1 (MQ=255)                                                                          cTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTg                                                                           >  8:147019/1‑149 (MQ=255)                                                                          cTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGACACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTg                                                                           >  8:237027/1‑149 (MQ=255)                                                                              aGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCaa                                                                       >  8:5668/1‑149 (MQ=255)                                                                                gATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGTCACATGGCCGGGCGTTTGCGGTGAAGCGCTACAGCATGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAg                                                                               <  7:226542/139‑1 (MQ=255)                                                                                gATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATg                                                                     >  6:242184/1‑149 (MQ=255)                                                                                gATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATg                                                                     <  7:121099/149‑1 (MQ=255)                                                                                gATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATg                                                                     >  1:132896/1‑149 (MQ=255)                                                                                gATGAGCATCTGCGTGCAAGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGAACAAGGCCGGGAGTTTGCGGTGAAGCGCTACAGCATGATCAGAACTCGTGATACGCAGTTTCAGGGTTCAg                                                                               >  8:226542/1‑139 (MQ=255)                                                                                  tGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTa                                                                                                                        >  7:104636/1‑96 (MQ=255)                                                                                  tGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTa                                                                                                                        <  8:104636/96‑1 (MQ=255)                                                                                   gAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGcc                                                                   <  6:317430/148‑1 (MQ=255)                                                                                       aTCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCt                                                              >  8:242064/1‑149 (MQ=255)                                                                                        tCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCtt                                                             >  8:380926/1‑149 (MQ=255)                                                                                         cTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTg                                                            >  3:47660/1‑149 (MQ=255)                                                                                             gTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAgg                                                                              >  6:91464/1‑127 (MQ=255)                                                                                             gTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAgg                                                                              <  5:91464/127‑1 (MQ=255)                                                                                               gCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCg                                                                                                      >  8:325790/1‑101 (MQ=255)                                                                                               gCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCg                                                                                                      <  7:325790/101‑1 (MQ=255)                                                                                               gCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCg                                                        >  3:292355/1‑147 (MQ=255)                                                                                               gCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCa                                                      >  2:189759/1‑149 (MQ=255)                                                                                                cACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGc                                                                         >  4:231102/1‑129 (MQ=255)                                                                                                cACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGc                                                                         <  3:231102/129‑1 (MQ=255)                                                                                                cACGCCAGGCAGCGGGAAGCGTTTTACCCGGCACCAAACTTAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCa                                                      <  1:135827/149‑1 (MQ=255)                                                                                                 aCGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGc                                                    <  2:11337/149‑1 (MQ=255)                                                                                                     cAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGcc                                                                   >  4:68180/1‑130 (MQ=255)                                                                                                     cAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGcc                                                                   <  3:68180/130‑1 (MQ=255)                                                                                                      aGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGtt                                               <  7:242064/149‑1 (MQ=255)                                                                                                       ggCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCa                                                                                             <  7:276827/102‑1 (MQ=255)                                                                                                       ggCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCa                                                                                             >  8:276827/1‑102 (MQ=255)                                                                                                         cAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcga                                            <  5:364423/149‑1 (MQ=255)                                                                                                           gCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgatc                                          <  5:287552/149‑1 (MQ=255)                                                                                                              ggAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCt                                                  <  2:316293/138‑1 (MQ=255)                                                                                                              ggAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCt                                                  >  1:316293/1‑138 (MQ=255)                                                                                                                aaGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAccc                                     >  2:395547/1‑149 (MQ=255)                                                                                                                    gTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTc                                 <  5:145234/149‑1 (MQ=255)                                                                                                                        tACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGc                              <  4:47660/148‑1 (MQ=255)                                                                                                                           ccGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGa                          >  6:335885/1‑149 (MQ=255)                                                                                                                           ccGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGa                          >  6:143609/1‑149 (MQ=255)                                                                                                                                agcaAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAgg                     <  7:147019/149‑1 (MQ=255)                                                                                                                                   gccc‑GAAAACGCCCGGCACCAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGc                                                           <  6:105764/105‑1 (MQ=255)                                                                                                                                   aaaC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGggcg                  >  1:319522/1‑149 (MQ=255)                                                                                                                                     aC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGggcggc                <  1:189759/149‑1 (MQ=255)                                                                                                                                     aC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCACAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGggcggc                >  3:133022/1‑149 (MQ=255)                                                                                                                                      ccgcaaaCGCCCGGCCTTGTGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATACGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGggcggc                >  7:62045/5‑149 (MQ=255)                                                                                                                                       cgcaaaCGCCCGGCCTTGTGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTc                                                                                 <  2:354369/80‑1 (MQ=25)                                                                                                                                       cgcaaaCGCCCGGCCTTGTGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTc                                                                                 >  1:354369/4‑83 (MQ=25)                                                                                                                                        gcaaaCGCCCGGCCTTGTGCCGGGCGTTTTCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGa              >  2:84610/3‑149 (MQ=255)                                                                                                                                        gcaaaCGCCCGGCCTTGTGCCGGGCGTTTTCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGa              >  2:90276/3‑149 (MQ=255)                                                                                                                                          aaaCGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATg            >  6:199269/1‑149 (MQ=255)                                                                                                                                            aCGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAcc                                      >  4:67183/1‑121 (MQ=255)                                                                                                                                            aCGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAcc                                      <  3:67183/121‑1 (MQ=255)                                                                                                                                              gCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGt        >  7:4049/1‑149 (MQ=255)                                                                                                                                              gCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGt        >  2:356661/1‑149 (MQ=255)                                                                                                                                               cccGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTc       <  7:380926/149‑1 (MQ=255)                                                                                                                                               cccGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTc       <  8:4049/149‑1 (MQ=255)                                                                                                                                                ccGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTcc      >  3:348829/1‑149 (MQ=255)                                                                                                                                                  ggCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCg    <  7:346648/149‑1 (MQ=255)                                                                                                                                                     acaAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGa              >  6:391532/1‑136 (MQ=255)                                                                                                                                                     acaAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGa              <  5:391532/136‑1 (MQ=255)                                                                                                                                                     acaAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGca  >  1:115564/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TGGACGATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAAC‑GAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCA  >  NC_002947/1054315‑1054609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑