Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,092,674 C→G 6.9% V51V (GTG→GTC) ‡ ptsH ← phosphocarrier protein HPr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,092,6740CG 6.9% 184.9 / 4.4 58V51V (GTG→GTC) ‡ptsHphosphocarrier protein HPr
Reads supporting (aligned to +/- strand):  ref base C (28/26);  new base G (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

CTGGTGCAAGCGCAGGTTTGCGAGGCCTATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGG  >  NC_002947/1092528‑1092822
                                                                                                                                                  |                                                                                                                                                    
cTGGTGCAAGCGCAGGTTTGCGAGGCCTATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCAc                                                                                                                                                    >  3:319661/1‑149 (MQ=255)
      cAAGCGCAGGTTTGCGAGGCCTATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCat                                                                                                                                              >  1:213043/1‑149 (MQ=255)
               gTTTGCGAGGCCTATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCtt                                                                                                                                     >  3:341982/1‑149 (MQ=255)
               gTTTGCGAGGCCTATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCtt                                                                                                                                     >  6:284111/1‑149 (MQ=255)
                  tGCGAGGCCTATTCTCCTTCGTAATAGATATTGTTGATCAGCTCGACCAGCGCGTCCATTGCGTCGCTGTCCTGCTCTCCCTCTGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACAGCCATGATGCTCTTGc                                                                                                                                   <  8:306596/148‑1 (MQ=255)
                   gCGAGGCCTATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATGGCGGTGATGATGctgg                                                                                                                                      >  4:270392/1‑142 (MQ=255)
                   gCGAGGCCTATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATGGCGGTGATGATGctgg                                                                                                                                      <  3:270392/144‑3 (MQ=255)
                           tATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAg                                                                                                                         >  6:291339/1‑149 (MQ=255)
                           tATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAg                                                                                                                         >  1:215076/1‑149 (MQ=255)
                           tATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAg                                                                                                                         >  2:107678/1‑149 (MQ=255)
                           tATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAg                                                                                                                         >  5:133985/1‑149 (MQ=255)
                             ttCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAg                                                                                                                         <  5:108507/147‑1 (MQ=255)
                             ttCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGACTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGc                                                                                                                        <  1:107678/148‑1 (MQ=255)
                                gCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTg                                                                                                                     <  6:28104/148‑1 (MQ=255)
                                           aGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGa                                                                                                         >  6:80533/1‑149 (MQ=255)
                                           aGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGa                                                                                                         >  3:278445/1‑149 (MQ=255)
                                               aTTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGaccca                                                                                                     <  6:84302/149‑1 (MQ=255)
                                                 tgttgATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGacccacc                                                                                                   >  7:289185/1‑149 (MQ=255)
                                                  gttgATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGacccacc                                                                                                   <  5:284111/148‑1 (MQ=255)
                                                   ttgATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGTCATCCACCAGCTTGTCCGGGGCGCGACCCACCCg                                                                                                 >  3:129556/1‑149 (MQ=255)
                                                   ttgATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGCCCCACCCg                                                                                                 >  2:135859/1‑149 (MQ=255)
                                                      aTCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGcc                                                                                                                                  >  8:30721/1‑113 (MQ=255)
                                                      aTCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGcc                                                                                                                                  <  7:30721/113‑1 (MQ=255)
                                                      aTCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATccac                                                                                                                            >  7:220917/1‑119 (MQ=255)
                                                      aTCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATccac                                                                                                                            <  8:220917/119‑1 (MQ=255)
                                                      aTCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTAc                                                                                              >  2:210671/1‑149 (MQ=255)
                                                       tCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATGGCGGTGATGATGctgg                                                                                                                                      <  6:44664/108‑3 (MQ=255)
                                                       tCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATGGCGGTGATGATGctgg                                                                                                                                      >  5:44664/1‑106 (MQ=255)
                                                       tCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTAcc                                                                                             >  4:237613/1‑149 (MQ=255)
                                                              gACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGTATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAgg                                                                                      <  8:295655/149‑1 (MQ=255)
                                                              gACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAg                                                                                       <  5:80533/148‑1 (MQ=255)
                                                                 cAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAgggg                                                                                    >  3:348444/1‑148 (MQ=255)
                                                                  aGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAgggg                                                                                    >  4:68613/1‑147 (MQ=255)
                                                                        tCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACGCGTACCTGGCAGGGGAAGcggcc                                                                            >  2:340143/1‑149 (MQ=255)
                                                                          cATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGcggccg                                                                           <  2:213043/148‑1 (MQ=255)
                                                                           aTGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGcggccggc                                                                         <  2:215076/149‑1 (MQ=255)
                                                                            tGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGcggccg                                                                           <  5:129244/146‑1 (MQ=255)
                                                                            tGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGcggccg                                                                           >  6:129244/1‑146 (MQ=255)
                                                                              gCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCca                                                                       >  4:226645/1‑148 (MQ=255)
                                                                                gTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCacac                                                                    <  4:278445/149‑1 (MQ=255)
                                                                                gTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCacac                                                                    <  3:237613/149‑1 (MQ=255)
                                                                                   gCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACcg                                                                  <  4:348444/148‑1 (MQ=255)
                                                                                    cTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACcgac                                                                <  1:210671/149‑1 (MQ=255)
                                                                                     tGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACcgac                                                                >  4:354840/1‑148 (MQ=255)
                                                                                           gCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTg                                                         <  5:291339/149‑1 (MQ=255)
                                                                                            cTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTg                                                         <  3:68613/148‑1 (MQ=255)
                                                                                                  cTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTgccg                                                  >  5:330995/1‑149 (MQ=255)
                                                                                                     ggTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGccc                                               <  6:87574/149‑1 (MQ=255)
                                                                                                           cAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCAt                                         <  8:289185/149‑1 (MQ=255)
                                                                                                                 cACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGTCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGAc                                   <  4:129556/149‑1 (MQ=255)
                                                                                                                      gggTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGc                              >  2:76629/1‑149 (MQ=255)
                                                                                                                       ggTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGc                              <  3:354840/148‑1 (MQ=255)
                                                                                                                          gCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCttgt                          >  3:236033/1‑149 (MQ=255)
                                                                                                                          gCCTTTGCCGGGGGCCACCATCATCACCCCCATGATGCTTTCTCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCttgt                          <  1:135859/149‑1 (MQ=255)
                                                                                                                            cTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCttgtt                         >  4:88943/1‑148 (MQ=255)
                                                                                                                               tGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTtgat                      <  3:226645/148‑1 (MQ=255)
                                                                                                                                       gCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGCAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTtgatga                    >  4:56275/1‑142 (MQ=255)
                                                                                                                                       gCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTtgatga                    <  3:56275/142‑1 (MQ=255)
                                                                                                                                              tcatcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCgcgg      <  3:88943/149‑1 (MQ=255)
                                                                                                                                                 tcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCgg                                             >  6:210740/1‑107 (MQ=255)
                                                                                                                                                 tcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCgg                                             <  5:210740/107‑1 (MQ=255)
                                                                                                                                                 tcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCCGCTTGTTGATGATGGTGATTTCgcgggcg   >  6:324575/1‑149 (MQ=255)
                                                                                                                                                 tcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTtgatga                    >  6:423952/1‑132 (MQ=255)
                                                                                                                                                 tcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTtgatga                    <  5:423952/132‑1 (MQ=255)
                                                                                                                                                 tcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGAtt            <  5:161615/140‑1 (MQ=255)
                                                                                                                                                 tcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGAtt            >  6:161615/1‑140 (MQ=255)
                                                                                                                                                 tcaCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCgcgggcg   >  7:312014/1‑149 (MQ=255)
                                                                                                                                                  caCCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCgcgggcgg  >  2:106972/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTGGTGCAAGCGCAGGTTTGCGAGGCCTATTCGCCTTCGTCAAAGAAATTGTTGATCAGCTCGACCAGCGCGTCCATGGCGTCGCTGTCCTGCTCGCCCTCGGTATGCAGGTGCACCTGGGTGCCTTTGCCTGCGGCCAGCATCATCACCGCCATGATGCTCTTGCCATCCACCAGCTTGTCCGGGGCGCGACCCACCCGTACCTGGCAGGGGAAGCGGCCGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGG  >  NC_002947/1092528‑1092822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑