| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 196,795 | G→C | 13.1% | T767T (ACG→ACC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 196,795 | 0 | G | C | 13.1% | 156.2 / 13.9 | 60 | T767T (ACG→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (29/23); new base C (4/4); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCT > NC_002947/196647‑196935 | aaaCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACg > 8:180978/1‑149 (MQ=255) ccGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCt > 1:405910/1‑146 (MQ=38) ccGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCa < 2:405910/148‑1 (MQ=38) ccGTTGAAGCCGGCAAGA‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGTCGGCAACATCGAACAGCTGACCCCGCACCCGACGCCGGCTCAGa > 5:333263/1‑149 (MQ=255) gCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGAt > 5:122016/1‑149 (MQ=37) aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGa > 4:180160/1‑149 (MQ=37) cccAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCg > 1:175350/1‑149 (MQ=34) gCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGAt < 5:53464/149‑1 (MQ=34) gCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGAt < 7:180978/149‑1 (MQ=34) cAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATg < 2:175350/149‑1 (MQ=34) ttCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATg < 5:136385/139‑1 (MQ=34) ttCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATg > 6:136385/1‑139 (MQ=34) tCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACcgc < 7:199977/148‑1 (MQ=34) cAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑Ac > 8:208380/1‑149 (MQ=34) ccGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGAc > 4:27621/1‑149 (MQ=34) acaacaACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGt > 3:112301/1‑148 (MQ=32) caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCgg > 7:154470/1‑83 (MQ=33) caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCgg < 8:154470/83‑1 (MQ=33) aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCAc < 7:208380/149‑1 (MQ=32) gTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAggc > 6:115658/1‑149 (MQ=32) ttCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCa < 4:15461/78‑1 (MQ=34) ttCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCa > 3:15461/1‑78 (MQ=34) gACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGgcggcg < 6:122016/149‑1 (MQ=32) cGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa > 5:92097/1‑149 (MQ=32) aGCGTCAACATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCa < 6:333263/148‑1 (MQ=17) gCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCAc < 4:240671/130‑1 (MQ=32) gCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCAc > 3:240671/1‑130 (MQ=32) gCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAcc < 3:27621/149‑1 (MQ=32) cATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCg > 2:384979/1‑149 (MQ=32) cATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCg > 6:269746/1‑149 (MQ=32) cATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCg > 7:41094/1‑149 (MQ=32) aTCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGt < 8:41094/149‑1 (MQ=32) gCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCg > 5:208980/1‑108 (MQ=32) gCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCg < 6:208980/108‑1 (MQ=32) ccACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGCCTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAg > 6:2497/1‑148 (MQ=21) ggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCGCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGc < 4:112301/149‑1 (MQ=17) ggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGc > 6:276728/1‑149 (MQ=32) gcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGcc < 3:180160/149‑1 (MQ=32) aaCTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGcc > 2:152757/1‑147 (MQ=32) cTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCGTGCCCAGa < 5:115658/149‑1 (MQ=17) ttCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTTACCCTGa > 8:150549/1‑133 (MQ=17) ttCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa < 7:150549/133‑1 (MQ=32) ttCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGAc > 4:251878/1‑149 (MQ=32) gAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGc < 1:384979/148‑1 (MQ=32) aaCAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGTCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg < 1:114096/149‑1 (MQ=17) aaCAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg > 3:232773/1‑149 (MQ=32) aaCAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg > 5:63092/1‑149 (MQ=32) aaCAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg > 7:276339/1‑149 (MQ=32) gCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGa < 4:232773/149‑1 (MQ=32) aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGt > 1:275946/1‑149 (MQ=32) aCCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACa < 5:298595/147‑1 (MQ=11) aCCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACa < 7:108991/147‑1 (MQ=11) cccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa < 3:362202/149‑1 (MQ=32) cccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa > 1:209846/1‑149 (MQ=32) cccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa > 4:362202/1‑149 (MQ=32) cccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGTACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGt > 1:323727/1‑148 (MQ=255) cccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa > 1:141527/1‑149 (MQ=17) ccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc < 5:96547/149‑1 (MQ=17) cGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc > 5:373094/1‑149 (MQ=17) cGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc > 6:181133/1‑149 (MQ=17) aaCCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGTCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCt < 3:218008/149‑1 (MQ=12) | AAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCG‑ACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCT > NC_002947/196647‑196935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |