Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,148,796 A→C 9.1% L150R (CTT→CGT)  PP_1007 ← transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,148,7960AC 9.1% 192.7 / 9.1 74L150R (CTT→CGT) PP_1007transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base A (31/34);  new base C (2/5);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

GGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCT  >  NC_002947/1148651‑1148943
                                                                                                                                                  |                                                                                                                                                   
ggggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAggt                                                                                                                                                  <  5:44720/149‑1 (MQ=255)
 gggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAggtg                                                                                                                                                 <  7:77218/149‑1 (MQ=255)
   gACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCTTACggtggt                                                                                                                                               <  7:54415/149‑1 (MQ=255)
    aCCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTc                                                                                                                                              <  5:294001/149‑1 (MQ=255)
    aCCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTc                                                                                                                                              <  3:70865/149‑1 (MQ=255)
     ccTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCTTACGGTGGTCg                                                                                                                                             <  4:398329/149‑1 (MQ=255)
     ccTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCg                                                                                                                                             <  6:326512/149‑1 (MQ=255)
      cTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCgg                                                                                                                                            >  2:236137/1‑149 (MQ=255)
         gAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCt                                                                                                                                         <  1:365378/149‑1 (MQ=255)
             tAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAg                                                                                                                                     >  5:317693/1‑149 (MQ=255)
              aaGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTAG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAgg                                                                                                                                    >  1:380841/1‑149 (MQ=255)
               aGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGgc                                                                                                                                   <  1:236137/149‑1 (MQ=255)
                gCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGgc                                                                                                                                   <  1:72489/149‑1 (MQ=255)
                gCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGgcg                                                                                                                                  <  7:174101/149‑1 (MQ=255)
                    cGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCTTACGGTGGTCGGc                                                                                                                                           <  6:193999/136‑1 (MQ=255)
                    cGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCTTACGGTGGTCGGc                                                                                                                                           >  5:193999/1‑136 (MQ=255)
                      cccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTACAGGCGCATggg                                                                                                                            >  3:24520/1‑149 (MQ=255)
                       ccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATggg                                                                                                                            >  1:18717/1‑148 (MQ=255)
                         tGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                         >  8:169453/1‑149 (MQ=255)
                          gTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGc                                                                                                                                       <  1:189924/134‑1 (MQ=255)
                           tAGCCGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAAGGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                         <  8:261467/147‑1 (MQ=255)
                                 gggggggACGTGGGGGCGGTCGTGGGGTTGGCTGGCTAATGCCTAATTGGTTTTGAGAAGCATTTTTGCGCGGTTTTCCAGTTGCTGGCGATAG‑CGCTGGCCGACCACGGTATGGTGGTGGGCCTGCAGGCGCATGGGCAGGTTACCGa                                                                                                                 <  2:380841/147‑1 (MQ=255)
                                    ggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACgg                                                                                                              >  2:205884/1‑149 (MQ=255)
                                     gcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGt                                                                                                             <  4:97465/149‑1 (MQ=255)
                                        aCCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGaa                                                                                                          >  7:393268/1‑149 (MQ=255)
                                             gcggcCGTCCTGGCGTTCGGTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGcc                                                                                                     <  6:317693/149‑1 (MQ=255)
                                             gcggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAg                                                                                                                                                    <  7:266919/102‑1 (MQ=255)
                                             gcggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAg                                                                                                                                                    >  8:266919/1‑102 (MQ=255)
                                             gcggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGcc                                                                                                     <  3:270066/149‑1 (MQ=255)
                                              cggccggcCTGGCGTTCGCTGGCGAATGCCGAATGGGGGTTGAGCAGCACTTTGGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGccc                                                                                                    <  6:337358/149‑1 (MQ=255)
                                               ggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGccc                                                                                                    >  8:304362/1‑148 (MQ=255)
                                                 ccGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGAc                                                                                                 <  7:169453/149‑1 (MQ=255)
                                                 ccGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGAc                                                                                                 >  7:334679/1‑149 (MQ=255)
                                                         gCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACcagcag                                                                                           >  1:229610/1‑147 (MQ=255)
                                                           gTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCTTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGgc                                                                                                                                   <  2:233349/105‑1 (MQ=255)
                                                           gTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGgc                                                                                                                                   >  1:233349/1‑105 (MQ=255)
                                                           gTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCagcagcagc                                                                                       <  7:304362/149‑1 (MQ=255)
                                                                cTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGc                                                                                  >  8:40326/1‑149 (MQ=255)
                                                                 tGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCATCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGc                                                                                  >  3:121943/1‑148 (MQ=255)
                                                                   gCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCATCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGc                                                                                  <  4:121943/146‑1 (MQ=255)
                                                                    ctaaTGCCTATTTGGTGTTGAGCAGCAGTTTTGCGCCGTCTTCCTTTTGCCGGCGCTGG‑CGCTCGCCGACCACCTTATGGTGGTGGGCCTACAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTg                                                                              <  4:24520/147‑1 (MQ=255)
                                                                    cGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTg                                                                              <  8:334679/149‑1 (MQ=255)
                                                                     gAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                         <  1:47067/105‑1 (MQ=255)
                                                                     gAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                         >  2:47067/1‑105 (MQ=255)
                                                                          ccGATTTGGTGTGGCCCACCACGTTTGCGCCGTCTGCCAGTCCCAGGCGCTGG‑CGCGCGCCGACCACCGTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAgg                                                                        <  7:139132/149‑1 (MQ=255)
                                                                          ccGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAg                                                                         <  3:168586/148‑1 (MQ=255)
                                                                           cGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                       <  7:40326/149‑1 (MQ=255)
                                                                           cGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                       >  1:207726/1‑149 (MQ=255)
                                                                           cGAATTGGTGTTGAGCAGCACTTTTGCGCCGGCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                       <  4:95096/149‑1 (MQ=255)
                                                                                 ggTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTc                                                                  <  1:205884/148‑1 (MQ=255)
                                                                                  gtgtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTc                                                                  >  4:203421/1‑147 (MQ=255)
                                                                                           agcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTgggtg                                                         >  1:351185/1‑143 (MQ=255)
                                                                                           agcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcg                                                       <  2:18717/149‑1 (MQ=255)
                                                                                              aCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAg                                                    <  8:393268/149‑1 (MQ=255)
                                                                                               cTTTTGCGCCGTGTTCCAGTTGCCGGGGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAgg                                                   <  2:207726/149‑1 (MQ=255)
                                                                                                ttttGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgc                                                  >  3:203483/1‑149 (MQ=255)
                                                                                                  ttGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcgc                                                >  2:224082/1‑149 (MQ=255)
                                                                                                     cgcCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCcg                                             >  8:183668/1‑149 (MQ=255)
                                                                                                             tCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGa                                     >  8:141827/1‑149 (MQ=255)
                                                                                                               cAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGAc                                    <  7:141827/148‑1 (MQ=255)
                                                                                                                 gTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                       <  7:115911/111‑1 (MQ=255)
                                                                                                                 gTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                       >  8:115911/1‑111 (MQ=255)
                                                                                                                    gCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGTGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGt                              >  2:50932/1‑149 (MQ=255)
                                                                                                                       ggcgctgg‑cgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTAACGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTtgc                           >  8:331906/1‑149 (MQ=255)
                                                                                                                        gcgctgg‑cgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCAGGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTtgct                          <  4:96181/149‑1 (MQ=255)
                                                                                                                              g‑cgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTgg                     <  5:348715/148‑1 (MQ=255)
                                                                                                                                      ccGACCACCTTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgc                                                  >  2:65578/1‑112 (MQ=255)
                                                                                                                                      ccGACCACCTTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgc                                                  <  1:65578/112‑1 (MQ=255)
                                                                                                                                         accaccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCg                                                                                 >  6:308285/1‑78 (MQ=255)
                                                                                                                                         accaccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCg                                                                                 <  5:308285/78‑1 (MQ=255)
                                                                                                                                            accGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGc       >  6:133748/1‑149 (MQ=255)
                                                                                                                                             ccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGGGGCCAGGGGTTTCCAGTGACTGCGCCGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGcc      >  6:321336/1‑149 (MQ=255)
                                                                                                                                              cGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGGCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCa     >  1:128687/1‑149 (MQ=255)
                                                                                                                                              cGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCATGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCa     >  4:318811/1‑149 (MQ=255)
                                                                                                                                                 aaGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCt  >  4:342272/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGG‑CGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCT  >  NC_002947/1148651‑1148943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑