Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,175,196	C→T	6.6%	L91F (CTC→TTC)	PP_1030 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,175,196	0	C	T	6.6%	221.2 / 3.9	76	L91F (CTC→TTC)	PP_1030	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/34);  new base T (3/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

GCCGCACCGGTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATT  >  NC_002947/1175050‑1175336                                                                                                                                                    |                                                                                                                                             gCCGCACCGGTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTc                                                                                                                                            >  2:41393/1‑149 (MQ=255)   cGCACCGGTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCg                                                                                                                                           >  1:300253/1‑148 (MQ=255)     cACCGGTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCTTCgag                                                                                                                                         >  1:204545/1‑147 (MQ=255)         ggTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGc                                                                                                                                    <  1:347447/149‑1 (MQ=255)             aCCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCTTCGAGGAAGccttc                                                                                                                                <  2:204545/149‑4 (MQ=255)              ccGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATGCCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGt                                                                                                                                <  1:101708/149‑1 (MQ=255)               cGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTg                                                                                                                              >  3:291409/1‑149 (MQ=255)                 cATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGcc                                                                                                                            >  8:110542/1‑149 (MQ=255)                  aTGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGccc                                                                                                                           <  8:174205/149‑1 (MQ=255)                     ggCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCTAAGAAGCCGGTTGCCCGGa                                                                                                                        <  2:177022/149‑1 (MQ=255)                      gCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCgaagaa                                                                                                                                      >  2:59778/1‑134 (MQ=255)                      gCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCgaagaa                                                                                                                                      <  1:59778/134‑1 (MQ=255)                       ccATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGt                                                                                                                      >  5:425373/1‑149 (MQ=255)                        cATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCgg                                                                                                                                 >  1:280127/1‑137 (MQ=255)                        cATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCgg                                                                                                                                 <  2:280127/137‑1 (MQ=255)                         aTGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGc                                                                                                                    <  6:425373/149‑1 (MQ=255)                          tGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGcc                                                                                                                   >  2:368557/1‑149 (MQ=255)                                 gcggcAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                            <  1:368557/149‑1 (MQ=255)                                  cggcAGGGGCGCTCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGag                                                                                                           <  8:381352/149‑1 (MQ=255)                                   ggcAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCTCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGATTGCCCGGAGAGCCGGCTCGagg                                                                                                          >  6:290918/1‑149 (MQ=255)                                     cAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGaggta                                                                                                        <  1:133410/149‑1 (MQ=255)                                     cAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                            >  7:328760/1‑145 (MQ=255)                                     cAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                            <  8:328760/145‑1 (MQ=255)                                       ggggCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGaggtagg                                                                                                      >  6:118267/1‑149 (MQ=255)                                            gcgcAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGc                                                                                                 <  7:110542/149‑1 (MQ=255)                                              gcAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCg                                                                                               <  4:291409/149‑1 (MQ=255)                                              gcAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCg                                                                                               >  2:12011/1‑149 (MQ=255)                                                aaaGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGTGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCgtt                                                                                             >  4:406689/1‑149 (MQ=255)                                                   gTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCgttgtt                                                                                          <  1:12011/149‑1 (MQ=255)                                                    tGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTc                                                                                         <  1:41393/149‑1 (MQ=255)                                                      gCCCTGCACCCGGAAAAACTGCTCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCAGCGAAGGCTTCCTCGAAGAAGCCGATTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCg                                                                                        <  5:290918/148‑1 (MQ=255)                                                       cccTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGaa                                                                                      <  6:336069/149‑1 (MQ=255)                                                        ccTGCACCCGGAAAAACTGCCCTTCGGTGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGaaa                                                                                     <  3:406689/149‑1 (MQ=255)                                                          tGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGc                                                                                   >  5:426090/1‑149 (MQ=255)                                                          tGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGc                                                                                   >  6:370100/1‑149 (MQ=255)                                                             aCCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCt                                                                                 >  3:338794/1‑148 (MQ=255)                                                             aCCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCt                                                                                 <  4:338794/148‑1 (MQ=255)                                                             aCCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTg                                                                                <  2:300253/149‑1 (MQ=255)                                                              cccGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAATCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTgg                                                                               <  7:123362/149‑1 (MQ=255)                                                               ccGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCgaa                                                                                                                                         <  6:314275/90‑1 (MQ=255)                                                               ccGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCgaa                                                                                                                                         >  5:314275/1‑90 (MQ=255)                                                                  gAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAaga                                                                           <  3:267048/149‑1 (MQ=255)                                                                  gAAAAACAGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACCCTGCATCTATACCCCCACCGAAGGCTTACACGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGAAGGTGAGCCGATCTTCGAAAGCCTGGaa                                                                             >  6:149397/1‑147 (MQ=255)                                                                             cTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCCCCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCgg                                                                >  8:322350/1‑149 (MQ=255)                                                                              ttCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCgg                                                                >  3:118403/1‑148 (MQ=255)                                                                              ttCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCATCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAACAGTGGATGCCgg                                                                >  3:287127/1‑148 (MQ=255)                                                                                    gAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAg                                                         >  8:137059/1‑149 (MQ=255)                                                                                      aCCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCg                                                       <  4:118403/149‑1 (MQ=255)                                                                                                 gCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTAcc                                            >  3:277598/1‑149 (MQ=255)                                                                                                  ccTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACttacctt                                           >  5:55435/1‑144 (MQ=255)                                                                                                    tGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGc                                         >  1:160113/1‑149 (MQ=255)                                                                                                     ggAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGCTGAGCCGTTGATCGAAAGCCTGGAAGACTGGATCCCGGCAGTGCGCGACACCTTGACCTGcc                                        >  2:40467/1‑149 (MQ=255)                                                                                                       aaGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCa                                      >  8:226527/1‑149 (MQ=255)                                                                                                            aTG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACtgtg                                 >  5:92374/1‑149 (MQ=255)                                                                                                                cTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCgg                              >  6:187281/1‑149 (MQ=255)                                                                                                                cTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCg                               <  6:426090/148‑1 (MQ=255)                                                                                                                 tCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGc                             >  1:411148/1‑149 (MQ=255)                                                                                                                 tCAAACCCTCCATCTATACACCCACCGAAGGCTTACTCGAAGAAGCCGGATGCCCGGAGTGCCCGCGCGAGGAAGGTGAGCCGTTCTACGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCAGCCCACTGTGCGGc                             >  8:285940/1‑149 (MQ=255)                                                                                                                   aaaCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCtt                           >  2:69211/1‑149 (MQ=255)                                                                                                                   aaaCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCt                            <  5:370100/148‑1 (MQ=255)                                                                                                                   aaaCGCTGATTTTATACCCCAACGCAAGGCTTCATCCATTAAGCGGGTTGCCCGTATGGCCGGCGGTAGGTAGGTTAGCCTTTTTTCTAAAGCCTGTAATAGTGTATGCCGGATTTTAGCTACAAC‑TTACCTGCCCACTGTGCGGCTTc                          <  6:55435/149‑1 (MQ=255)                                                                                                                    aaCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTc                          <  5:187281/149‑1 (MQ=255)                                                                                                                      cGCTGCATCTATACCCCCACCGACGGCTTCCTCGAAGAAGGCGGTTGCCCGGAGTGACGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGa                        <  8:270895/149‑1 (MQ=255)                                                                                                                        cTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACt                                                 <  7:390040/122‑1 (MQ=255)                                                                                                                        cTGCATCTATACCACCACCGAAGCCATCCTCGAAGAAGCCGGTTGCCCGGACTGCCGGCGCGAGGTACCTTAGCCGTTTTTCGAAAGCCTGGAAGAGTGGAAGCAGGCAGTGAGCGACAACt                                                 >  8:390040/1‑122 (MQ=255)                                                                                                                           cATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATg                   <  7:226527/149‑1 (MQ=255)                                                                                                                               tataCCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACAt               <  7:137059/149‑1 (MQ=255)                                                                                                                                 ctcccccACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATc              <  1:146501/146‑1 (MQ=255)                                                                                                                                    ccccACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATc              >  2:146501/1‑145 (MQ=255)                                                                                                                                      ccACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGc        <  4:277598/149‑1 (MQ=255)                                                                                                                                        aCCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACGGTGCGGCTGCGAAGATGACATCAATGGCtt      >  7:393679/1‑149 (MQ=255)                                                                                                                                            ccggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCtt                           <  6:351143/122‑1 (MQ=255)                                                                                                                                            ccggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCtt                           >  5:351143/3‑124 (MQ=255)                                                                                                                                            ccggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCAtt  >  6:420748/3‑149 (MQ=255)                                                                                                                                                 ttCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACtt                                                >  7:319435/1‑98 (MQ=255)                                                                                                                                                 ttCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACtt                                                <  8:319435/98‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             GCCGCACCGGTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATT  >  NC_002947/1175050‑1175336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑