Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,175,201	A→G	5.6%	E92E (GAA→GAG)	PP_1030 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,175,201	0	A	G	5.6%	208.6 / 2.2	72	E92E (GAA→GAG)	PP_1030	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/32);  new base G (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

GGTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGCCAT  >  NC_002947/1175058‑1175349                                                                                                                                                 |                                                                                                                                                     ggTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGc                                                                                                                                                 <  1:347447/149‑1 (MQ=255)     aCCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCTTCGAGGAAGccttc                                                                                                                                             <  2:204545/149‑4 (MQ=255)      ccGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATGCCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGt                                                                                                                                             <  1:101708/149‑1 (MQ=255)       cGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTg                                                                                                                                           >  3:291409/1‑149 (MQ=255)         cATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGcc                                                                                                                                         >  8:110542/1‑149 (MQ=255)          aTGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGccc                                                                                                                                        <  8:174205/149‑1 (MQ=255)             ggCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCTAAGAAGCCGGTTGCCCGGa                                                                                                                                     <  2:177022/149‑1 (MQ=255)              gCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCgaagaa                                                                                                                                                   <  1:59778/134‑1 (MQ=255)              gCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCgaagaa                                                                                                                                                   >  2:59778/1‑134 (MQ=255)               ccATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGt                                                                                                                                   >  5:425373/1‑149 (MQ=255)                cATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCgg                                                                                                                                              >  1:280127/1‑137 (MQ=255)                cATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCgg                                                                                                                                              <  2:280127/137‑1 (MQ=255)                 aTGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGc                                                                                                                                 <  6:425373/149‑1 (MQ=255)                  tGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGcc                                                                                                                                >  2:368557/1‑149 (MQ=255)                         gcggcAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                                         <  1:368557/149‑1 (MQ=255)                          cggcAGGGGCGCTCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGag                                                                                                                        <  8:381352/149‑1 (MQ=255)                           ggcAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCTCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGATTGCCCGGAGAGCCGGCTCGagg                                                                                                                       >  6:290918/1‑149 (MQ=255)                             cAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGaggta                                                                                                                     <  1:133410/149‑1 (MQ=255)                             cAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                                         <  8:328760/145‑1 (MQ=255)                             cAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                                         >  7:328760/1‑145 (MQ=255)                               ggggCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGaggtagg                                                                                                                   >  6:118267/1‑149 (MQ=255)                                    gcgcAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGc                                                                                                              <  7:110542/149‑1 (MQ=255)                                      gcAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCg                                                                                                            >  2:12011/1‑149 (MQ=255)                                      gcAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCg                                                                                                            <  4:291409/149‑1 (MQ=255)                                        aaaGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGTGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCgtt                                                                                                          >  4:406689/1‑149 (MQ=255)                                           gTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCgttgtt                                                                                                       <  1:12011/149‑1 (MQ=255)                                            tGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTc                                                                                                      <  1:41393/149‑1 (MQ=255)                                              gCCCTGCACCCGGAAAAACTGCTCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCAGCGAAGGCTTCCTCGAAGAAGCCGATTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCg                                                                                                     <  5:290918/148‑1 (MQ=255)                                               cccTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGaa                                                                                                   <  6:336069/149‑1 (MQ=255)                                                ccTGCACCCGGAAAAACTGCCCTTCGGTGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGaaa                                                                                                  <  3:406689/149‑1 (MQ=255)                                                  tGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGc                                                                                                >  6:370100/1‑149 (MQ=255)                                                  tGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGc                                                                                                >  5:426090/1‑149 (MQ=255)                                                     aCCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCt                                                                                              >  3:338794/1‑148 (MQ=255)                                                     aCCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCt                                                                                              <  4:338794/148‑1 (MQ=255)                                                     aCCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTg                                                                                             <  2:300253/149‑1 (MQ=255)                                                      cccGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAATCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTgg                                                                                            <  7:123362/149‑1 (MQ=255)                                                       ccGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCgaa                                                                                                                                                      <  6:314275/90‑1 (MQ=255)                                                       ccGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCgaa                                                                                                                                                      >  5:314275/1‑90 (MQ=255)                                                          gAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAaga                                                                                        <  3:267048/149‑1 (MQ=255)                                                          gAAAAACAGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACCCTGCATCTATACCCCCACCGAAGGCTTACACGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGAAGGTGAGCCGATCTTCGAAAGCCTGGaa                                                                                          >  6:149397/1‑147 (MQ=255)                                                                     cTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCCCCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCgg                                                                             >  8:322350/1‑149 (MQ=255)                                                                      ttCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCgg                                                                             >  3:118403/1‑148 (MQ=255)                                                                      ttCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCATCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAACAGTGGATGCCgg                                                                             >  3:287127/1‑148 (MQ=255)                                                                            gAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAg                                                                      >  8:137059/1‑149 (MQ=255)                                                                              aCCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCg                                                                    <  4:118403/149‑1 (MQ=255)                                                                                         gCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTAcc                                                         >  3:277598/1‑149 (MQ=255)                                                                                          ccTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACttacctt                                                        >  5:55435/1‑144 (MQ=255)                                                                                            tGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGc                                                      >  1:160113/1‑149 (MQ=255)                                                                                             ggAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGCTGAGCCGTTGATCGAAAGCCTGGAAGACTGGATCCCGGCAGTGCGCGACACCTTGACCTGcc                                                     >  2:40467/1‑149 (MQ=255)                                                                                               aaGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCa                                                   >  8:226527/1‑149 (MQ=255)                                                                                                    aTG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACtgtg                                              >  5:92374/1‑149 (MQ=255)                                                                                                        cTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCgg                                           >  6:187281/1‑149 (MQ=255)                                                                                                        cTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCg                                            <  6:426090/148‑1 (MQ=255)                                                                                                         tCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGc                                          >  1:411148/1‑149 (MQ=255)                                                                                                         tCAAACCCTCCATCTATACACCCACCGAAGGCTTACTCGAAGAAGCCGGATGCCCGGAGTGCCCGCGCGAGGAAGGTGAGCCGTTCTACGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCAGCCCACTGTGCGGc                                          >  8:285940/1‑149 (MQ=255)                                                                                                           aaaCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCtt                                        >  2:69211/1‑149 (MQ=255)                                                                                                           aaaCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCt                                         <  5:370100/148‑1 (MQ=255)                                                                                                           aaaCGCTGATTTTATACCCCAACGCAAGGCTTCATCCATTAAGCGGGTTGCCCGTATGGCCGGCGGTAGGTAGGTTAGCCTTTTTTCTAAAGCCTGTAATAGTGTATGCCGGATTTTAGCTACAAC‑TTACCTGCCCACTGTGCGGCTTc                                       <  6:55435/149‑1 (MQ=255)                                                                                                            aaCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTc                                       <  5:187281/149‑1 (MQ=255)                                                                                                              cGCTGCATCTATACCCCCACCGACGGCTTCCTCGAAGAAGGCGGTTGCCCGGAGTGACGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGa                                     <  8:270895/149‑1 (MQ=255)                                                                                                                cTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACt                                                              <  7:390040/122‑1 (MQ=255)                                                                                                                cTGCATCTATACCACCACCGAAGCCATCCTCGAAGAAGCCGGTTGCCCGGACTGCCGGCGCGAGGTACCTTAGCCGTTTTTCGAAAGCCTGGAAGAGTGGAAGCAGGCAGTGAGCGACAACt                                                              >  8:390040/1‑122 (MQ=255)                                                                                                                   cATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATg                                <  7:226527/149‑1 (MQ=255)                                                                                                                       tataCCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACAt                            <  7:137059/149‑1 (MQ=255)                                                                                                                         ctcccccACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATc                           <  1:146501/146‑1 (MQ=255)                                                                                                                            ccccACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATc                           >  2:146501/1‑145 (MQ=255)                                                                                                                              ccACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGc                     <  4:277598/149‑1 (MQ=255)                                                                                                                                aCCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACGGTGCGGCTGCGAAGATGACATCAATGGCtt                   >  7:393679/1‑149 (MQ=255)                                                                                                                                    ccggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCtt                                        >  5:351143/3‑124 (MQ=255)                                                                                                                                    ccggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCtt                                        <  6:351143/122‑1 (MQ=255)                                                                                                                                    ccggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCAtt               >  6:420748/3‑149 (MQ=255)                                                                                                                                         ttCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACtt                                                             >  7:319435/1‑98 (MQ=255)                                                                                                                                         ttCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACtt                                                             <  8:319435/98‑1 (MQ=255)                                                                                                                                             tCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAg      >  3:316950/1‑149 (MQ=255)                                                                                                                                             tCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAg      >  6:317200/1‑149 (MQ=255)                                                                                                                                             tCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAg      >  6:140523/1‑149 (MQ=255)                                                                                                                                               gaagaaGCCGTTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGACGAGTGGATGCCGGCAGTGATCGACAACTTTACCTGCCCACTGTGCGGCTTCGATGATGACATCAATGGCTTCATTTACCTGCATcc    <  5:118267/149‑1 (MQ=255)                                                                                                                                               gaagaaGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTTGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGaa                                    <  2:307693/117‑1 (MQ=255)                                                                                                                                               gaagaaGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTGGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGaa                                    >  1:307693/1‑117 (MQ=255)                                                                                                                                               gaagaaGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCTGGCTGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGc     >  1:128758/1‑148 (MQ=255)                                                                                                                                               gaagaaGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGc                                             >  3:138536/1‑108 (MQ=255)                                                                                                                                               gaagaaGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGc                                             <  4:138536/108‑1 (MQ=255)                                                                                                                                               gaagaaGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACGTGGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGc     >  1:137006/1‑148 (MQ=255)                                                                                                                                                 agaagCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGCCAt  <  1:69211/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GGTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTCGGCGAACCGGTCAACGGCCTGGAAGTGATG‑CTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGCCAT  >  NC_002947/1175058‑1175349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑