| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,189,035 | A→G | 20.5% | intergenic (+25/+17) | PP_1038 → / ← tatC‑I | hypothetical protein/Sec‑independent protein translocase protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,189,035 | 0 | A | G | 20.5% | 99.1 / 23.1 | 57 | intergenic (+25/+17) | PP_1038/tatC‑I | hypothetical protein/Sec‑independent protein translocase protein |
| Reads supporting (aligned to +/- strand): ref base A (20/23); new base G (4/7); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 | |||||||||||
GTGGAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCCGGCGGTGTCAG > NC_002947/1188893‑1189183 | gTGGAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCcg > 7:206149/1‑149 (MQ=255) gAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCcgc‑g > 7:7660/1‑148 (MQ=255) ggTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCGTGCCCGC‑Gaata < 1:99664/149‑3 (MQ=255) ggTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCGTGCCCGC‑Gaata < 7:174349/149‑3 (MQ=255) aGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCGTGCCCGC‑Gaatagccgt < 8:279375/149‑8 (MQ=255) aGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGccc < 4:134136/149‑1 (MQ=255) gCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGccc > 6:118333/1‑148 (MQ=255) cTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTa < 1:90038/149‑1 (MQ=255) tggtggtggCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTa > 6:403702/1‑148 (MQ=255) tggtggCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCCCTTTCGCGGGCGTGCCCGC‑Gaatagccgt > 8:204684/1‑137 (MQ=255) tggtggCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCCCTTTCGCGGGCGTGCCCGC‑Gaatagccgt < 7:204684/144‑8 (MQ=255) tggCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCa > 2:168989/1‑149 (MQ=255) tGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCca > 7:245723/1‑149 (MQ=255) gAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGCGGAAAGGGCCCTACAATCCAGCCACaa < 1:212822/149‑1 (MQ=255) tGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTcc > 1:382309/1‑149 (MQ=255) aTTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCtt > 1:193585/1‑149 (MQ=255) tgCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCt < 8:245723/149‑1 (MQ=255) cgcTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTc < 5:403702/149‑1 (MQ=255) gcTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGc > 7:131801/1‑142 (MQ=255) gcTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGc < 8:131801/142‑1 (MQ=255) aaGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACa < 1:168989/148‑1 (MQ=255) ccACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCca > 7:145565/1‑145 (MQ=255) ccACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCca < 8:145565/145‑1 (MQ=255) cACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGc < 3:39235/149‑1 (MQ=255) cccGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCa > 1:350950/1‑149 (MQ=255) tACTAGACGCCAGCATATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCaa < 8:384034/149‑1 (MQ=255) aGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCGTGCCCGC‑Gaa < 4:359473/69‑1 (MQ=255) aaGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCAcc > 3:161946/1‑149 (MQ=255) cTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCcaca > 1:83256/1‑149 (MQ=255) gcggcTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GTAAGGGCCCTACAATcc < 7:309692/69‑1 (MQ=255) gcggcTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GGAAGGGCCCTACAATcc > 8:309692/1‑69 (MQ=255) gcTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGc > 7:364064/1‑94 (MQ=255) gcTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGc < 8:364064/94‑1 (MQ=255) tCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCa < 5:128312/147‑1 (MQ=255) tCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCa > 6:128312/1‑147 (MQ=255) tCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACt < 4:147260/149‑1 (MQ=255) tCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACt > 3:147260/1‑149 (MQ=255) gCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAg > 6:121328/1‑113 (MQ=255) gCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAg < 5:121328/113‑1 (MQ=255) gCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGc < 2:382309/149‑1 (MQ=255) cGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCa < 8:206149/149‑1 (MQ=255) cGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCa < 2:350950/149‑1 (MQ=255) gAACGCATCACGG‑CTATTCGCGGGCTCGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTc < 8:7660/148‑1 (MQ=255) gAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTc < 2:193585/148‑1 (MQ=255) aaCGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTg < 7:76453/149‑1 (MQ=255) aCGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGc < 4:161946/149‑1 (MQ=255) cGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCg < 2:83256/149‑1 (MQ=255) gccctttCGCGGGCGTGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCa < 2:156833/124‑1 (MQ=255) gccctttCGCGGGCGTGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCa > 1:156833/6‑129 (MQ=255) g‑CTATTCGCGGGCCCGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACAt > 5:118352/1‑149 (MQ=255) cctttCGCGGGCGTGCCCGC‑GAATGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATc > 5:131353/4‑149 (MQ=255) tATTCGCGGGCCCGCCCGC‑GAAAGGGCCCTACAATCGAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTGCACAAGCCAGCAACCCCAATGAGGGAACAGCACCCACATCGGCACTACCAGCATCGTCTGCGAAAACACAt > 1:132051/1‑149 (MQ=255) tttCGCGGGCGTGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACa < 4:260819/103‑1 (MQ=255) tttCGCGGGCGTGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACa > 3:260819/2‑104 (MQ=255) gcgGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑At‑ct > 2:362472/1‑94 (MQ=255) gcgGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑At‑ct < 1:362472/94‑1 (MQ=255) cACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCCGGCGgtgt > 7:344667/1‑147 (MQ=255) aCGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCCGGCGGTGTCAg > 1:211448/1‑149 (MQ=255) | GTGGAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGG‑CTATTCGCGGGCACGCCCGC‑GAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCC‑AT‑CTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCCGGCGGTGTCAG > NC_002947/1188893‑1189183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |