Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,208,810 G→A 8.4% S64L (TCG→TTG)  PP_1058 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,208,8100GA 8.4% 133.8 / 2.2 48S64L (TCG→TTG) PP_1058hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base A (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GCTTGAGCAGGCGTGGCAGGAATTCACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACGGGGGGACTTGGGTTGTGGCGTCTCGGCCGGAGGTATCTC  >  NC_002947/1208664‑1208955
                                                                                                                                                  |                                                                                                                                                 
gcTTGAGCAGGCGTGGCAGGAATTCACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGAt                                                                                                                                                 <  7:219059/149‑1 (MQ=255)
  ttGAGCAGGCGTGGCAGGAATTCACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTg                                                                                                                                               >  5:391345/1‑149 (MQ=255)
     aGCAGGCGTGGCAGGAATTCACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCa                                                                                                                                             <  8:345723/148‑1 (MQ=255)
           cGTGGCAGGAATTCACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCaga                                                                                                                                      <  2:319872/149‑1 (MQ=255)
               gCAGGAATTCACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTa                                                                                                                                  >  5:50236/1‑149 (MQ=255)
                     aTTCACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACgg                                                                                                                               >  1:146307/1‑146 (MQ=255)
                        cACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTc                                                                                                                          <  2:146307/148‑1 (MQ=255)
                         aCCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTAc                                                                                                                                 >  1:255860/1‑140 (MQ=255)
                         aCCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTAc                                                                                                                                 <  2:255860/140‑1 (MQ=255)
                         aCCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGt                                                                                                                        >  2:340906/1‑149 (MQ=255)
                          cccAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGt                                                                                                                        >  3:382653/1‑148 (MQ=255)
                          cccAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTc                                                                                                                       <  3:324553/149‑1 (MQ=255)
                           ccAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTa                                                                                                                                  >  4:68712/1‑137 (MQ=255)
                           ccAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTa                                                                                                                                  <  3:68712/137‑1 (MQ=255)
                           ccAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCa                                                                                                                      <  6:391345/149‑1 (MQ=255)
                                       aGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAaca                                                                                                          >  5:216423/1‑149 (MQ=255)
                                        gCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAacac                                                                                                         <  6:216423/149‑1 (MQ=255)
                                           cAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGa                                                                                                      <  5:64472/149‑1 (MQ=255)
                                              gCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATgg                                                                                                   >  6:26642/1‑149 (MQ=255)
                                                gTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATAcgcg                                                                                                                <  7:341157/134‑1 (MQ=255)
                                                gTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATAcgcg                                                                                                                >  8:341157/1‑134 (MQ=255)
                                                gTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGc                                                                                                  >  4:143171/1‑148 (MQ=255)
                                                       gAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAgcg                                                                                          >  6:258554/1‑149 (MQ=255)
                                                        aaCGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAgcgc                                                                                         <  2:37402/149‑1 (MQ=255)
                                                           ggCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGccac                                                                                      <  1:206174/149‑1 (MQ=255)
                                                               cGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCTGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACcagc                                                                                  <  7:310897/149‑1 (MQ=255)
                                                                        ggTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCaggatgagg                                                                         <  4:382653/149‑1 (MQ=255)
                                                                        ggTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCaggatgag                                                                          >  1:194156/1‑148 (MQ=255)
                                                                               gatgGCATTGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCag                                                                                                                                       >  8:282354/1‑80 (MQ=255)
                                                                               gatgGCATTGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCag                                                                                                                                       <  7:282354/80‑1 (MQ=255)
                                                                                                        aGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACg                                         >  2:264372/1‑149 (MQ=255)
                                                                                                         gCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACgg                                        <  5:258554/149‑1 (MQ=255)
                                                                                                           gAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACgggg                                      <  1:340906/149‑1 (MQ=255)
                                                                                                            ctttggTCATTGTACTGGCCAGTTTGCTGAAATCGCGCTATTGCAGTTCAGATGTACTGAATTCGTCATACGCGGCAACACCGTTGGCAACCAGCGCCACCAGTAGGATGAGTATTATCAGCCGCCACCATAGCCGACGCTGACgggg                                      <  2:370449/144‑1 (MQ=255)
                                                                                                            actAGGTCAGTGTACTGGCCAGTTTGCTGTACTCGCGGGATTGCAGTTCTGAGGTACGGATTTCGTCATTCGCGGCAACAGCGTTGGCAACCAGCGCCACCAGCAGGATGAGTATGATCAGCGGCCACCATAGCCGACGCTGACgggg                                      <  3:284871/146‑1 (MQ=255)
                                                                                                            aaTAGGTCAGTGTACTGCCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCACCCAGCGCCACCAGAAGCATGAGGATGATCAGCCGCCACCAAAGCCGACGCTGACcggg                                      >  4:284871/1‑148 (MQ=255)
                                                                                                               aGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACgggg                                      >  1:370449/1‑145 (MQ=255)
                                                                                                                      tgtACTGGCCAGTTTGCTGAACTCGCGCAATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCaggatgagga                                                                        <  4:246689/104‑1 (MQ=255)
                                                                                                                      tgtACTGGCCAGTTTGCTGAACTCGCGCAATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCaggatgagga                                                                        <  4:241334/104‑1 (MQ=255)
                                                                                                                      tgtACTGGCCAGTTTGCTGAACTCGCGCAATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCaggatgagga                                                                        >  3:246689/1‑104 (MQ=255)
                                                                                                                      tgtACTGGCCAGTTTGCTGAACTCGCGCAATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCaggatgagga                                                                        >  3:241334/1‑104 (MQ=255)
                                                                                                                        tACTGGCCAGTTTGATGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACGGGGGGACTTGGGTtgt                         >  8:75107/1‑149 (MQ=255)
                                                                                                                          cTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACGGGGGGACTTGGGTtgtg                        <  1:264372/148‑1 (MQ=255)
                                                                                                                           tGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACGGGGGGACTTGGGTTGTGGc                      >  2:410399/1‑149 (MQ=255)
                                                                                                                             gCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCagg                                                                               <  2:11188/90‑1 (MQ=255)
                                                                                                                             gCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACGGGGGGACTTGGGTTGTGGc                      <  5:26642/147‑1 (MQ=255)
                                                                                                                                aGTTTGCTGACCTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGTATGATCAGCCGCCACCATAGCCGACGc                                              <  7:282773/120‑1 (MQ=255)
                                                                                                                                aGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGc                                              >  8:282773/1‑120 (MQ=255)
                                                                                                                                               cgcgATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACGGGGGGACTTGGGTTGTGGCGTCTCGGCCGGAGGTTtctc  <  6:50236/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTTGAGCAGGCGTGGCAGGAATTCACCCAGGGCGCTATAGCCCAGGCGTTTGTCGAACGGCCCGTCACCGGGGTAGATGATGGCATCGCTGGGACCGGGTTGCAGCGAATAGGTCAGTGTACTGGCCAGTTTGCTGAACTCGCGCGATTGCAGTTCAGAGGTACGGAATTCGTCATACGCGGCAACACCGATGGCAACCAGCGCCACCAGCAGGATGAGGATGATCAGCCGCCACCATAGCCGACGCTGACGGGGGGACTTGGGTTGTGGCGTCTCGGCCGGAGGTATCTC  >  NC_002947/1208664‑1208955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑