Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,215,084 G→T 6.8% Y625* (TAC→TAA) ‡ PP_1061 ← ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,215,0840GT 6.8% 190.3 / 4.1 59Y625* (TAC→TAA) ‡PP_1061ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base T (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

CAGGGCCAGGCCCCAGGCGCGGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCA  >  NC_002947/1214937‑1215231
                                                                                                                                                    |                                                                                                                                                   
cAGGGCCAGGCCCCAGGCGCGGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGCAAGGGCGCCAAGCACTTCACGGGTGCGGGCCAgg                                                                                                                                                     <  1:257041/149‑1 (MQ=255)
   ggCCAGGCCCCAGGCGCGGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGt                                                                                                                                                 <  5:338299/149‑1 (MQ=255)
       aGGCCCCAGGCGCGGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAg                                                                                                                                             <  2:355966/149‑1 (MQ=255)
         gCCACAGGCGCGGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGc                                                                                                                                            >  4:111309/1‑148 (MQ=255)
          ccccAGGCGCGGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCaa                                                                                                                                          <  1:148031/149‑1 (MQ=255)
           cccAGGCGCGGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAAc                                                                                                                                         >  4:114803/1‑149 (MQ=255)
                 cgcgGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCTGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGccc                                                                                                                                   <  1:82443/149‑1 (MQ=255)
                 cgcgGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGTTGCTCGACTACCTGGccc                                                                                                                                   <  3:227877/149‑1 (MQ=255)
                      ttGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGcac                                                                                                                               >  4:345717/1‑148 (MQ=255)
                                        ggTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGTTGCTCGACTACCTGGCCCGcacccgtg                                                                                                                          >  4:111239/1‑130 (MQ=38)
                                        ggTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGTTGCTCGACTACCTGGCCCGcacccgtg                                                                                                                          <  3:111239/135‑6 (MQ=38)
                                           gaatgaatGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAgg                                                                                                          >  2:74751/1‑148 (MQ=255)
                                              tgaatgaTCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGc                                                                                                      <  3:111309/149‑1 (MQ=255)
                                               gaatgaTCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGcc                                                                                                     >  1:188447/1‑149 (MQ=255)
                                                         ttGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGtt                                                                                                                <  1:393896/128‑1 (MQ=255)
                                                         ttGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGtt                                                                                                                >  2:393896/1‑128 (MQ=255)
                                                              tCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGt                                                                                      <  1:74751/149‑1 (MQ=255)
                                                                ccgccgGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTAc                                                                                    >  8:73522/1‑149 (MQ=255)
                                                                       aCTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCAcc                                                                             <  2:188447/149‑1 (MQ=255)
                                                                           aTCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCgtgt                                                                         >  4:344751/1‑149 (MQ=255)
                                                                           aTCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCgt                                                                           <  2:59158/147‑1 (MQ=255)
                                                                           aTCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGAAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGc                                                                                          <  2:36842/132‑1 (MQ=255)
                                                                           aTCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGAAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGc                                                                                          >  1:36842/1‑132 (MQ=255)
                                                                            tCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCt                                                                                                    <  4:319248/121‑1 (MQ=255)
                                                                            tCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCt                                                                                                    >  3:319248/1‑121 (MQ=255)
                                                                            tCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTc                                                                        <  3:344751/149‑1 (MQ=255)
                                                                            tCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTc                                                                        <  3:345717/149‑1 (MQ=255)
                                                                             cGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTcg                                                                       >  2:173313/1‑149 (MQ=255)
                                                                              gAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTcgc                                                                      >  8:95873/1‑149 (MQ=255)
                                                                                   aaCGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCt                                                                 >  8:191188/1‑149 (MQ=255)
                                                                                     cggtCCAGCACCATCGTATCCAGCGAGGGC‑AGGGCTCCATGCTCTTCCCGGGTGCGGGCCAGGTAGTCGAGCATCTGGCCCGCACTAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTAGCTCCACCGTGTCGCCGCCTGc                                                               <  8:165667/146‑1 (MQ=255)
                                                                                       ttCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGc                                                               >  5:381473/1‑147 (MQ=255)
                                                                                       ttCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCa                                                             <  2:31086/149‑1 (MQ=255)
                                                                                         ccctCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGGCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAgc                                                           >  6:291610/4‑149 (MQ=255)
                                                                                          cATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAgcg                                                          >  6:402452/1‑149 (MQ=255)
                                                                                           aTCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCt                                                                                                    >  7:90378/1‑106 (MQ=255)
                                                                                           aTCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCt                                                                                                    <  8:90378/106‑1 (MQ=255)
                                                                                                cAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGtt                                                    >  7:6413/1‑149 (MQ=255)
                                                                                                      aTCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGt                                              >  3:264549/1‑149 (MQ=255)
                                                                                                       tCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGt                                              <  4:264549/148‑1 (MQ=255)
                                                                                                         cTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGa                                           <  7:95873/149‑1 (MQ=255)
                                                                                                          tGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGAt                                          >  7:232517/1‑149 (MQ=255)
                                                                                                           gCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGAtt                                         >  2:40105/1‑149 (MQ=255)
                                                                                                              agggc‑agggcGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGtt                                                    <  8:328487/135‑1 (MQ=255)
                                                                                                              agggc‑agggcGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGtt                                                    >  7:328487/1‑135 (MQ=255)
                                                                                                                  c‑agggcGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCTCCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCt                                  <  7:73522/149‑1 (MQ=255)
                                                                                                                                    cACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCAcggc                  <  8:6413/148‑1 (MQ=255)
                                                                                                                                     aCGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGa                                           <  7:189980/122‑1 (MQ=255)
                                                                                                                                     aCGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGa                                           >  8:189980/1‑122 (MQ=255)
                                                                                                                                     aCGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCAcggcg                 <  1:40105/148‑1 (MQ=255)
                                                                                                                                        tgTGCGGGCCAGTTGCTCGACTACCTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCAcggcgg                >  6:397686/2‑146 (MQ=39)
                                                                                                                                         gTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCCGCCGTGGCCCGGCTGCAgg            <  6:381473/149‑2 (MQ=255)
                                                                                                                                             gggCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTg                                                                                                   >  4:149584/1‑58 (MQ=255)
                                                                                                                                             gggCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTg                                                                                                   <  3:149584/58‑1 (MQ=255)
                                                                                                                                             gggCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTg                                                                >  7:195191/1‑93 (MQ=255)
                                                                                                                                             gggCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTg                                                                <  8:195191/93‑1 (MQ=255)
                                                                                                                                             gggCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGc        >  5:356888/1‑149 (MQ=255)
                                                                                                                                              ggCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCt       >  6:175906/1‑149 (MQ=255)
                                                                                                                                               gCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTc      <  1:173313/149‑1 (MQ=255)
                                                                                                                                               gCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCACGCCTGCAGCCGGGCCACGGCGGCAGACAGCTc      <  7:191188/149‑1 (MQ=255)
                                                                                                                                                   ggTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCa  <  5:291610/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGGGCCAGGCCCCAGGCGCGGTTGATGCGGCTACCGTAGGGTGAATGAATGATCAGTTGGGTCCCGCCGGACTCATCGAAAAAACGTTCCATCACCAGCGTATCCTGCGAGGGC‑AGGGCGCCAAGCACTTCACGGGTGCGGGCCAGGTAGTCGAGCAACTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCA  >  NC_002947/1214937‑1215231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑