Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,231,194 C→A 6.9% V507L (GTG→TTG)  glpD ← aerobic glycerol‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,231,1940CA 6.9% 230.3 / 5.8 72V507L (GTG→TTG) glpDaerobic glycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (32/35);  new base A (2/3);  total (34/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

AAGCACTGAAGGCTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCCAGCAGGTAATCCACCTCGCGGGT  >  NC_002947/1231046‑1231335
                                                                                                                                                    |                                                                                                                                               
aaGCACTGAAGGCTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCAc                                                                                                                                                 <  3:256994/149‑1 (MQ=255)
  gCACTGAAGGCTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCt                                                                                                                                               >  7:2503/1‑149 (MQ=255)
        aaGGCTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCg                                                                                                                                           <  3:378662/147‑1 (MQ=255)
         aGGCTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGc                                                                                                                                        >  2:96814/1‑149 (MQ=255)
         aGGCTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCAAGGTGCGGGc                                                                                                                                        <  2:83679/149‑1 (MQ=255)
           gCTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCtt                                                                                                                                      <  5:73370/149‑1 (MQ=255)
            cTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCCGCGCGGCTATTCGCCCGCAAGGTGCGGGcgaa                                                                                                                                     <  3:145469/149‑4 (MQ=255)
               aTTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCttgt                                                                                                                                    <  7:181616/147‑1 (MQ=255)
               aTTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCttgt                                                                                                                                    <  2:227630/147‑1 (MQ=255)
                tttCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGc                                                                                                                                 <  8:2503/149‑1 (MQ=255)
                    gATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGa                                                                                                                              <  5:14521/148‑1 (MQ=255)
                       cGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGc                                                                                                                                 <  5:240116/142‑1 (MQ=255)
                       cGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGc                                                                                                                                 >  6:240116/1‑142 (MQ=255)
                          agcgagcgGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCt                                                                                                                       >  2:302296/1‑149 (MQ=255)
                            cgagcgGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCttg                                                                                                                                     <  1:185343/133‑1 (MQ=255)
                            cgagcgGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCttg                                                                                                                                     <  1:185347/133‑1 (MQ=255)
                            cgagcgGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCttg                                                                                                                                     >  2:185347/1‑133 (MQ=255)
                            cgagcgGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCttg                                                                                                                                     >  2:185343/1‑133 (MQ=255)
                               gcgGGCATTGTTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAgg                                                                                                                  <  5:38252/149‑1 (MQ=255)
                                cgGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAggg                                                                                                                 <  8:26606/149‑1 (MQ=255)
                                cgGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAggg                                                                                                                 <  8:20564/149‑1 (MQ=255)
                                  ggCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGc                                                                                                                >  4:213265/1‑148 (MQ=255)
                                     aTTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATGCGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATc                                                                                                            <  6:131232/149‑1 (MQ=255)
                                         ctcgcgcGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCt                                                                                                        >  6:191801/4‑149 (MQ=255)
                                         cTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCGCCGTAGGTATCAGGCTGCGCGGCTATACGCCAGCACCTTGCGGGCTTGTTGCAGATACGACTCCATGGCCATCTGCt                                                                                                        >  3:245795/1‑149 (MQ=255)
                                         cTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCt                                                                                                        >  7:106913/1‑149 (MQ=255)
                                              gcgGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTgc                                                                                                   >  7:189905/1‑149 (MQ=255)
                                                 gTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGcgcg                                                                                                <  4:106662/149‑1 (MQ=255)
                                                       gcgTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGc                                                                                          <  5:191801/149‑1 (MQ=255)
                                                         gTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGa                                                                                        >  5:215337/1‑149 (MQ=255)
                                                                        gcTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGt                                                                       <  6:215337/149‑1 (MQ=255)
                                                                        gcTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGt                                                                       <  1:52101/149‑1 (MQ=255)
                                                                                aaTACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGcc                                                               <  4:251192/149‑1 (MQ=255)
                                                                                aaTACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGc                                                                >  2:11518/1‑148 (MQ=255)
                                                                                     gCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTgc                                                                                                   <  7:388089/110‑1 (MQ=255)
                                                                                     gCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTgc                                                                                                   >  8:388089/1‑110 (MQ=255)
                                                                                       ccccTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGa                                                            >  7:71974/1‑145 (MQ=255)
                                                                                       ccccTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGa                                                            <  8:71974/145‑1 (MQ=255)
                                                                                       ccccTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAAt                                                        >  2:385907/1‑149 (MQ=255)
                                                                                        cccTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAGCGCCCAGCTT‑GGTGCGACGCCAGATAAt                                                        <  1:11518/149‑1 (MQ=255)
                                                                                        cccTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATg                                                       >  6:169610/1‑149 (MQ=255)
                                                                                          cTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCCGCGACCG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGt                                                      >  4:122049/1‑148 (MQ=255)
                                                                                              aCGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACa                                                                                      <  7:80461/114‑1 (MQ=255)
                                                                                              aCGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACa                                                                                      >  8:80461/1‑114 (MQ=255)
                                                                                                   tCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTgcg                                                                                                  >  8:330082/1‑97 (MQ=255)
                                                                                                   tCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTgcg                                                                                                  <  7:330082/97‑1 (MQ=255)
                                                                                                      agagGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCATATAATGTCAGc                                                  >  3:264456/1‑140 (MQ=255)
                                                                                                      agagGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCATATAATGTCAGc                                                  <  4:264456/140‑1 (MQ=255)
                                                                                                       gagGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAAt                                                        >  7:212673/1‑133 (MQ=255)
                                                                                                       gagGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAAt                                                        <  8:212673/133‑1 (MQ=255)
                                                                                                             ttCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCAt                                  >  6:417707/1‑149 (MQ=255)
                                                                                                               cACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATg                                                       <  3:275344/126‑1 (MQ=255)
                                                                                                               cACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATg                                                       >  4:275344/1‑126 (MQ=255)
                                                                                                                aCCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtc                                <  8:189905/148‑1 (MQ=255)
                                                                                                                aCCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtc                                <  6:402719/148‑1 (MQ=255)
                                                                                                                 ccGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTGCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtcct                              >  1:155870/1‑149 (MQ=255)
                                                                                                                    tAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGcc                                     >  8:290006/1‑139 (MQ=255)
                                                                                                                    tAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGcc                                     <  7:290006/139‑1 (MQ=255)
                                                                                                                       gTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtcctcctc                          >  4:63728/1‑147 (MQ=255)
                                                                                                                        tATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCACGGCCATCTGCTCCTTCGCGGTCAGCGACAG‑GCCCATCTTCGGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtcctcctcc                         <  1:302296/148‑1 (MQ=255)
                                                                                                                         aTCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCcag                       >  3:29243/1‑148 (MQ=255)
                                                                                                                          tCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCcagca                     <  4:29243/149‑1 (MQ=255)
                                                                                                                           cAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCcagcag                    >  2:46621/1‑149 (MQ=255)
                                                                                                                               cTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCCAGCAGGTaa                >  7:381320/1‑149 (MQ=255)
                                                                                                                                tGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCCAGCAGGTAAt               >  7:386803/1‑149 (MQ=255)
                                                                                                                                   gcgGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtc                                >  5:230681/1‑129 (MQ=255)
                                                                                                                                   gcgGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtc                                <  6:230681/129‑1 (MQ=255)
                                                                                                                                         acaagCCCGCAAGGTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCCAGCAGGTAATCCACCTcgc      >  2:320989/5‑149 (MQ=255)
                                                                                                                                          ttCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCCAGCAGGTAATCCACCTcgcg     <  8:112408/149‑1 (MQ=255)
                                                                                                                                            cGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCCAGCAGGTAATCCACCTCGCgg    <  1:385907/148‑1 (MQ=255)
                                                                                                                                             gCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCCAGCAGGTAATCCACCTCGCGGGt  <  1:46621/149‑1 (MQ=255)
                                                                                                                                             gCCCGCAAGGTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtcctcctc                          >  7:364146/1‑125 (MQ=255)
                                                                                                                                             gCCCGCAAGGTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATtcctcctc                          <  8:364146/125‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AAGCACTGAAGGCTGATTTCGATCGCAGCGAGCGGGCATTGCTGGCGCGGTTGGCGCGTGAAGCCTACGAGCGCTGGGCGAATACGCCCCCTTGACGGATCAAGAGGGCTTCACCGTAGGTATCAGGCTGCGCGGCTATTCGCCCGCACCTTGCGGGCTTGTTGCAGATACGCCTCCAGGGCCATCTGCTCCTGCGCGGTCAGCGACAG‑GCCCAGCTT‑GGTGCGACGCCAGATAATGTCAGCGGTTTGCTCTGCCCATTCCTCCTCCAGCAGGTAATCCACCTCGCGGGT  >  NC_002947/1231046‑1231335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑