Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,243,739 T→C 9.6% intergenic (+28/+71) tsaA → / ← rnt peroxiredoxin/ribonuclease T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,243,7390TC 9.6% 255.8 / 14.0 94intergenic (+28/+71)tsaA/rntperoxiredoxin/ribonuclease T
Reads supporting (aligned to +/- strand):  ref base T (41/44);  new base C (4/5);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

TTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCGGTT  >  NC_002947/1243595‑1243882
                                                                                                                                                |                                                                                                                                                
ttCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTg                                                                                                                                              >  5:424372/1‑149 (MQ=255)
   cACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGcc                                                                                                                                           <  3:254744/149‑1 (MQ=255)
          agCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGttttt                                                                                                                                    <  6:424372/149‑1 (MQ=255)
           gCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGtttttt                                                                                                                                   >  7:213474/1‑149 (MQ=255)
             aCGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTa                                                                                                                                 <  1:109116/149‑1 (MQ=255)
             aCGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCACGAAGGCCGGttttttca                                                                                                                                 <  1:210282/149‑3 (MQ=255)
              cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTAt                                                                                                                                <  8:106882/149‑1 (MQ=255)
              cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTAt                                                                                                                                >  1:56989/1‑149 (MQ=255)
              cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTAt                                                                                                                                <  6:156043/149‑1 (MQ=255)
              cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTCGCCGGTTTTTTTAt                                                                                                                                <  7:351708/149‑1 (MQ=255)
               ggCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATg                                                                                                                               >  2:328185/1‑149 (MQ=255)
               ggCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATg                                                                                                                               >  5:298512/1‑149 (MQ=255)
                 cGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGcc                                                                                                                             >  5:409807/1‑149 (MQ=255)
                 cGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAACGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGACCCAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCACGAAGGCCGGTTTTTTCATGca                                                                                                                             <  5:267898/149‑2 (MQ=38)
                     gtgtGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGAATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTcc                                                                                                                         >  6:268094/1‑149 (MQ=255)
                      tgtgCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑t                                                                                                                       <  8:213474/149‑1 (MQ=255)
                          cccTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCAt                                                                                                                                >  1:137746/1‑137 (MQ=38)
                          cccTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCAt                                                                                                                                >  1:142442/1‑137 (MQ=38)
                          cccTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCAt                                                                                                                                <  2:142442/137‑1 (MQ=38)
                          cccTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCAt                                                                                                                                <  2:137746/137‑1 (MQ=38)
                            cTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCg                                                                                                                 <  7:111809/149‑1 (MQ=255)
                                gaaCTGGAAAAAAGGCGTCAAGGGCATGATCGCTTCGCCGGATGGCGTTGCTGCCTACCTGTGTGAGAACTCTGGCTAGCTGTATTTGGTGCGTGCTTCATTAACCCGTCCTTCTTGGCCGGTTTTTTTATGCCTTCG‑TGTGCCGGCct                                                                                                             <  4:258930/148‑1 (MQ=255)
                                  aCTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCctct                                                                                                           >  6:94987/1‑149 (MQ=255)
                                    tGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTc                                                                                                         >  2:54080/1‑149 (MQ=255)
                                       aaaaaaGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTcgcg                                                                                                      >  3:284148/1‑149 (MQ=255)
                                        aaaaaGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCgg                                                                                                     >  1:74431/1‑149 (MQ=255)
                                         aaaaGGCGACAAGGGCATGAAAGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCggg                                                                                                    >  4:266018/1‑149 (MQ=255)
                                            aGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGgcgc                                                                                                 <  5:268094/149‑1 (MQ=255)
                                              gCGACAACGGCATGAACGCTTCGCCCGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGgcgcgc                                                                                               <  2:18784/149‑1 (MQ=255)
                                                gACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGccc                                                                                             >  5:416653/1‑149 (MQ=255)
                                                  cAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGgcgcgc                                                                                               <  3:172321/145‑1 (MQ=255)
                                                  cAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGATGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGgcgcgc                                                                                               >  4:172321/1‑145 (MQ=255)
                                                     gggCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGaa                                                                                        <  5:94987/149‑1 (MQ=255)
                                                       gCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATg                                                                                      >  5:351783/1‑149 (MQ=255)
                                                       gCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATg                                                                                      >  4:293268/1‑149 (MQ=255)
                                                        cATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGc                                                                                     <  2:56989/149‑1 (MQ=255)
                                                        cATGAACGCTTCGCCTGAAGGCGTTGCTGCCTAACTGAGCGACAACGCGGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTTGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATg                                                                                      <  1:54080/149‑1 (MQ=255)
                                                           gAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCtt                                                                                  >  6:320222/1‑149 (MQ=255)
                                                            aacccgtCGCCTGAAGGCGTTGCTGCCTACCTGAGCGACAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCttt                                                                                 <  7:254784/143‑1 (MQ=255)
                                                            aaCGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCAAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTc                                                                                                                                                 >  6:336770/1‑86 (MQ=255)
                                                               gCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGc                                                                              <  8:35172/149‑1 (MQ=255)
                                                                    gCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATc                                                                         <  3:293268/149‑1 (MQ=255)
                                                                    gCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATc                                                                         <  6:409807/149‑1 (MQ=255)
                                                                       tGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGtttt                                                                                                                                     >  4:47270/1‑87 (MQ=25)
                                                                       tGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGtttt                                                                                                                                     <  3:47270/87‑1 (MQ=25)
                                                                            gCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCGTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGtt                                                                  >  5:424375/1‑148 (MQ=255)
                                                                              gTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAAt                                                                                       <  6:172553/125‑1 (MQ=255)
                                                                              gTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAAt                                                                                       >  5:172553/1‑125 (MQ=255)
                                                                                  ctgcCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATc                                                            <  8:387028/148‑1 (MQ=255)
                                                                                     ccTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCgcgc                                                        >  1:208055/1‑149 (MQ=255)
                                                                                     ccTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCgcgc                                                        <  2:74431/149‑1 (MQ=255)
                                                                                       tACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCgcgc                                                        >  3:295148/1‑147 (MQ=255)
                                                                                          cTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAgcc                                                   >  2:234462/1‑149 (MQ=255)
                                                                                           tGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCa                                                                             <  2:19451/122‑1 (MQ=255)
                                                                                           tGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAgccg                                                  >  3:113976/1‑149 (MQ=255)
                                                                                               cGAGAACGCTGGCAAGCTGTAATTGCTGTGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCa                                              >  5:138761/1‑149 (MQ=255)
                                                                                               cGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCa                                              <  1:234462/149‑1 (MQ=255)
                                                                                                gagaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAt                                             >  8:232038/1‑149 (MQ=255)
                                                                                                gagaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAt                                             <  6:416653/149‑1 (MQ=255)
                                                                                                gagaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCACCCAt                                             >  6:316934/1‑149 (MQ=255)
                                                                                                 agaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTATAAGATAAATCGTTGAAATCGCTCCAGCCCGCCAtt                                            >  8:304971/1‑149 (MQ=255)
                                                                                                 agaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtt                                            <  6:424375/149‑1 (MQ=255)
                                                                                                 agaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtt                                            >  7:97910/1‑149 (MQ=255)
                                                                                                  gaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAttt                                           <  7:232038/149‑1 (MQ=255)
                                                                                                    aCGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttct                                         <  4:295148/149‑1 (MQ=255)
                                                                                                       cTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttctttc                                      <  4:113976/149‑1 (MQ=255)
                                                                                                       cTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCACCCAtttctttc                                      <  5:316934/149‑1 (MQ=255)
                                                                                                        tGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGc                                                                                     >  2:352423/1‑101 (MQ=255)
                                                                                                        tGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGc                                                                                     <  1:352423/101‑1 (MQ=255)
                                                                                                          gCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCa                                    <  5:320222/148‑1 (MQ=255)
                                                                                                               cTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGAGCCAGCCGCCCATTTCTTTCCAGCGGtt                              <  6:351783/149‑1 (MQ=255)
                                                                                                                tGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGtt                              >  2:192479/1‑148 (MQ=255)
                                                                                                                 gTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGa                            >  6:7308/1‑149 (MQ=255)
                                                                                                                  tAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGAc                           >  6:172256/1‑149 (MQ=255)
                                                                                                                    aTTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGccc                                                                                             <  3:172363/81‑1 (MQ=255)
                                                                                                                    aTTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGccc                                                                                             >  4:172363/1‑81 (MQ=255)
                                                                                                                        ctgcTTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGc                      <  7:304971/148‑1 (MQ=255)
                                                                                                                        ctgcGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAAt                                                                      <  7:163364/100‑1 (MQ=255)
                                                                                                                        ctgcGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAAt                                                                      >  8:163364/1‑100 (MQ=255)
                                                                                                                           cGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCg                    <  5:172256/147‑1 (MQ=255)
                                                                                                                           cGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCa                  <  2:208055/149‑1 (MQ=255)
                                                                                                                               cATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAAc              <  6:138761/149‑1 (MQ=255)
                                                                                                                                aTGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttctt                                        >  1:161028/1‑122 (MQ=255)
                                                                                                                                  gAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttctt                                        <  5:20454/120‑1 (MQ=255)
                                                                                                                                  gAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttctt                                        >  6:20454/1‑120 (MQ=255)
                                                                                                                                  gAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGc           >  5:306627/1‑149 (MQ=255)
                                                                                                                                  gAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCG‑ACGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCAGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGc           >  2:81321/1‑148 (MQ=255)
                                                                                                                                   aaaaaaCCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttctttc                                      >  6:129393/1‑121 (MQ=255)
                                                                                                                                   aaaaaaCCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttctttc                                      <  5:129393/121‑1 (MQ=255)
                                                                                                                                   aaaaaaCCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGtt                              <  6:137954/129‑1 (MQ=255)
                                                                                                                                   aaaaaaCCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGtt                              >  5:137954/1‑129 (MQ=255)
                                                                                                                                    aaaaaCCGGCCACGAAGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCt          >  2:279232/1‑148 (MQ=255)
                                                                                                                                           ggCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCGGtt  <  5:7308/149‑1 (MQ=255)
                                                                                                                                           ggCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCGGtt  <  8:97910/149‑1 (MQ=255)
                                                                                                                                               ttCGTGTCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTGTTCGCGGGCGCGCCCGCGAATGCCTTTTGCAGATCAATCGTTGAAATCGCTCCAGCCTCCCATTTCTTTCCAGCGGTTGACGATGCCg                    <  4:126074/127‑1 (MQ=255)
                                                                                                                                               ttCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCAGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCAAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCg                    >  3:126074/1‑127 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCC‑TGTGCCGGCCTCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCGGTT  >  NC_002947/1243595‑1243882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑