Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,253,429 G→C 7.7% intergenic (‑75/+32) PP_1096 ← / ← metG electron transport complex protein/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,253,4290GC 7.7% 160.6 / 4.4 52intergenic (‑75/+32)PP_1096/metGelectron transport complex protein/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base C (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

GCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCG  >  NC_002947/1253288‑1253577
                                                                                                                                             |                                                                                                                                                    
gCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCg                                                                                                                                               >  7:45080/1‑149 (MQ=255)
   gTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTc                                                                                                                                            <  6:122634/149‑1 (MQ=255)
    tCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGCCGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAg                                                                                                                                                   >  6:415381/1‑141 (MQ=255)
    tCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAg                                                                                                                                                   <  5:415381/141‑1 (MQ=255)
       gAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCca                                                                                                                                         >  3:76047/1‑148 (MQ=255)
       gAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCca                                                                                                                                         >  8:160726/1‑148 (MQ=255)
                   gTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGc                                                                                                                                                     <  1:294827/124‑1 (MQ=255)
                   gTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGc                                                                                                                                                     >  2:294827/1‑124 (MQ=255)
                      attgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGc                                                                                                                         >  6:34958/1‑149 (MQ=255)
                       ttgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCAGGCAGGCCGCTCCCACAATGGCCCGCAGGGCg                                                                                                                        <  2:231363/149‑1 (MQ=255)
                          atCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAAt                                                                                                                                     >  8:72599/1‑133 (MQ=255)
                          atCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAAt                                                                                                                                     <  6:227458/133‑1 (MQ=255)
                          atCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAAt                                                                                                                                     <  7:72599/133‑1 (MQ=255)
                          atCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGt                                                                                                                      >  8:31810/1‑148 (MQ=255)
                              gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGCAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 <  7:147647/149‑1 (MQ=255)
                                  ggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATg                                                                                                             >  7:346425/1‑149 (MQ=255)
                                    cgcTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATgcg                                                                                                           >  7:351275/1‑149 (MQ=255)
                                    cgcTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATgcg                                                                                                           <  2:400809/149‑1 (MQ=255)
                                         aTCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctg                                                                                                        <  8:351275/147‑1 (MQ=255)
                                             ggACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcctgg                                                                                                  <  8:45080/149‑1 (MQ=255)
                                              gACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcctggc                                                                                                 >  8:16699/1‑149 (MQ=255)
                                                 cAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTGACTTGATGCGCTGGCCTGGCTTg                                                                                              >  4:115342/1‑149 (MQ=255)
                                                         aGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 <  6:59326/122‑1 (MQ=255)
                                                         aGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 >  5:59326/1‑122 (MQ=255)
                                                         aGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCt                                                                                      >  8:59995/1‑149 (MQ=255)
                                                            cattcatGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCAGCTCCCACAATGGCCCGCAGTGCGGTTACTTGATGCGATGGCCTGGCTTGGCGCCGCTGTc                                                                                   >  3:293939/1‑149 (MQ=255)
                                                                       tGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTcagca                                                                        <  8:99683/149‑1 (MQ=255)
                                                                        gACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTcagcag                                                                       >  4:161659/1‑149 (MQ=255)
                                                                          cGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGt                                                                     <  5:70580/149‑1 (MQ=255)
                                                                                ccGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGAttt                                                               <  3:161659/149‑1 (MQ=255)
                                                                                ccGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGAttt                                                               >  6:54099/1‑149 (MQ=255)
                                                                                      aTCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCAc                                                         <  7:160726/149‑1 (MQ=255)
                                                                                         ggggCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGc                                                      >  7:171411/1‑149 (MQ=255)
                                                                                          gggCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTTGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGc                                                      <  4:76047/148‑1 (MQ=255)
                                                                                          gggCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGcc                                                     <  7:16699/149‑1 (MQ=255)
                                                                                            gCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAg                                                   >  1:269378/1‑149 (MQ=255)
                                                                                                  gTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCg                                              <  8:346425/148‑1 (MQ=255)
                                                                                                       ggTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGcc                                        <  8:171411/149‑1 (MQ=255)
                                                                                                            gACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTg                                                                                              <  6:354715/90‑1 (MQ=255)
                                                                                                            gACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTg                                                                                              >  5:354715/1‑90 (MQ=255)
                                                                                                            gACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGAc                                   >  3:155344/1‑149 (MQ=255)
                                                                                                                 gAAGGGGCCTGCGTAATTGTGGGAGCGGGCAAGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGc                              >  1:41613/1‑149 (MQ=255)
                                                                                                                      ggCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCg                         <  4:155344/149‑1 (MQ=255)
                                                                                                                             gTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGc                  <  5:34958/149‑1 (MQ=255)
                                                                                                                                    tgGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTc                          >  8:60566/1‑134 (MQ=255)
                                                                                                                                    tgGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTc                          <  7:60566/134‑1 (MQ=255)
                                                                                                                                    tgGGAGCGGCCTGCCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGa                       <  2:14783/137‑1 (MQ=255)
                                                                                                                                    tgGGAGCGGCCTGCCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGa                       >  1:14783/1‑137 (MQ=255)
                                                                                                                                     gggAGCGGCCTGCCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCggg                                                                               >  8:170953/1‑80 (MQ=38)
                                                                                                                                     gggAGCGGCCTGCCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCggg                                                                               <  7:170953/80‑1 (MQ=38)
                                                                                                                                      ggAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAgcgc          <  2:41613/148‑1 (MQ=255)
                                                                                                                                       gAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCAt        >  4:357927/1‑149 (MQ=255)
                                                                                                                                           gggCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTc    >  8:228034/1‑149 (MQ=255)
                                                                                                                                            ggCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTcc   >  1:216191/1‑149 (MQ=255)
                                                                                                                                             gCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCg  >  3:184917/1‑149 (MQ=255)
                                                                                                                                             gCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCg  <  4:184917/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCG  >  NC_002947/1253288‑1253577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑