Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,273,453 G→A 5.1% A41V (GCC→GTC)  PP_1114 ← SEC‑C domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,273,4530GA 5.1% 305.5 / 3.7 98A41V (GCC→GTC) PP_1114SEC‑C domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (47/46);  new base A (3/2);  total (50/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

Only 100 of 101 total aligned reads displayed.

TCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCG  >  NC_002947/1273314‑1273600
                                                                                                                                           |                                                                                                                                                   
tCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTa                                                                                                                                            >  6:219710/1‑149 (MQ=255)
  gcgGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATc                                                                                                                                          <  3:285794/149‑1 (MQ=255)
        gcgcCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTcc                                                                                                                                    >  1:86025/1‑149 (MQ=255)
          gcCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCtg                                                                                                                                  >  8:182456/1‑149 (MQ=255)
             cGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCtggtg                                                                                                                               <  1:21266/149‑1 (MQ=255)
              gCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCtggtgg                                                                                                                              >  3:187123/1‑149 (MQ=255)
                  ccACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGt                                                                                                                          >  7:96417/1‑149 (MQ=255)
                   cagaGTGTTCGAGCCATCTCTCAGATTTTTGTCGCGTTAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTg                                                                                                                         <  8:50126/146‑1 (MQ=255)
                    acaGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTgg                                                                                                                        >  4:82172/1‑149 (MQ=255)
                      aGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGtt                                                                                                                      <  1:284349/149‑1 (MQ=255)
                         gtTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTg                                                                                                                     >  4:401021/1‑147 (MQ=255)
                          ttCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCa                                                                                                                   <  5:280532/148‑1 (MQ=255)
                             gAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAg                                                                                                                  >  1:14259/1‑146 (MQ=255)
                             gAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAg                                                                                                                  <  2:14259/146‑1 (MQ=255)
                             gAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGc                                                                                                               >  1:293887/1‑149 (MQ=255)
                               gCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCgg                                                                                                             <  2:293887/149‑1 (MQ=255)
                               gCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGCCCTGAAGGACGTTggcc                                                                                                                                             <  7:83339/117‑4 (MQ=255)
                               gCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGCCCTGAAGGACGTTggcc                                                                                                                                             >  8:83339/1‑114 (MQ=255)
                                ccATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCggg                                                                                                            <  6:44149/149‑1 (MQ=255)
                                  aTCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGcc                                                                                                          >  6:86194/1‑149 (MQ=255)
                                    ctctcAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCgt                                                                                                        >  4:361163/1‑149 (MQ=255)
                                     tctcAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGtt                                                                                                                      >  8:311043/1‑134 (MQ=255)
                                     tctcAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGtt                                                                                                                      <  7:311043/134‑1 (MQ=255)
                                       tcAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCgtgt                                                                                                      >  7:16589/1‑148 (MQ=255)
                                       tcAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCgtgt                                                                                                      >  3:214777/1‑148 (MQ=255)
                                       tcAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCgt                                                                                                        <  4:157703/146‑1 (MQ=255)
                                       tcAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTa                                                                                                     >  7:271164/1‑149 (MQ=255)
                                        cAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTa                                                                                                     >  4:15961/1‑148 (MQ=255)
                                         aGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGc                                                                                                                                       <  8:30763/113‑1 (MQ=255)
                                         aGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGc                                                                                                                                       >  7:30763/1‑113 (MQ=255)
                                         aGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCgtgt                                                                                                      <  3:82172/146‑1 (MQ=255)
                                         aGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACa                                                                                                   >  3:327605/1‑149 (MQ=255)
                                          gATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACAt                                                                                                  <  4:187123/149‑1 (MQ=255)
                                          gATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACAt                                                                                                  >  8:86994/1‑149 (MQ=255)
                                            tttttATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGc                                                                                                                                               <  5:389067/102‑1 (MQ=255)
                                            tttttATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGc                                                                                                                                               >  6:389067/1‑102 (MQ=255)
                                             ttttATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGc                                                                                               >  3:987/1‑149 (MQ=255)
                                              tttATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGcc                                                                                              >  3:117877/1‑149 (MQ=255)
                                                tATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCcgt                                                                                            >  7:294397/1‑149 (MQ=255)
                                                         aGTCGTTCAGGCGCCGTGTCACTTCTTGTACTTCTTCTCGCTACCGCACTGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCcgtggtcgtgg                                                                                    <  8:271164/148‑1 (MQ=255)
                                                          gTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCgtggtg                                                                                  <  8:16589/149‑1 (MQ=255)
                                                          gTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGGCgtggtg                                                                                  <  5:86194/149‑1 (MQ=255)
                                                               tCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCgtggtggtg                                                                               >  6:123982/1‑147 (MQ=255)
                                                               tCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCgtggtggtg                                                                               <  5:123982/147‑1 (MQ=255)
                                                                   gcgcCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATg                                                                         >  6:105677/1‑149 (MQ=255)
                                                                   gcgcCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATg                                                                         >  7:377875/1‑149 (MQ=255)
                                                                   gcgcCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATg                                                                         <  2:86025/149‑1 (MQ=255)
                                                                    cgcCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATgg                                                                        <  5:219710/149‑1 (MQ=255)
                                                                    cgcCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATgg                                                                        <  3:361163/149‑1 (MQ=255)
                                                                      ccGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGc                                                                       >  8:18730/1‑148 (MQ=255)
                                                                         tGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATg                                                                   >  1:180718/1‑149 (MQ=255)
                                                                          ggCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGc                                                                                                                    <  2:93864/99‑1 (MQ=255)
                                                                          ggCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGc                                                                                                                    >  1:93864/1‑99 (MQ=255)
                                                                           gCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGt                                                                 <  7:18730/149‑1 (MQ=255)
                                                                             aCTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTg                                                               <  4:327605/149‑1 (MQ=255)
                                                                              cttcttGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGc                                                              <  7:86994/149‑1 (MQ=255)
                                                                                  ttGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGt                                                           >  1:163960/1‑148 (MQ=255)
                                                                                    gTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAg                                                        >  5:399051/1‑149 (MQ=255)
                                                                                     tACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAg                                                        <  4:214777/148‑1 (MQ=255)
                                                                                       cttcttCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGa                                                                              >  3:131634/1‑124 (MQ=255)
                                                                                       cttcttCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGa                                                                              <  4:131634/124‑1 (MQ=255)
                                                                                       cttcttCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGc                                                     <  2:126899/149‑1 (MQ=255)
                                                                                          cttctCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCAt                                                  >  3:324807/1‑149 (MQ=255)
                                                                                            tctcGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATg                                                 >  8:350236/1‑148 (MQ=255)
                                                                                             ctcGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGa                                                >  4:6997/1‑148 (MQ=255)
                                                                                                gCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCCGTCAGGGcgggg                                                 <  5:337744/144‑5 (MQ=255)
                                                                                                gCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCCGTCAGGGcgggg                                                 >  6:337744/1‑140 (MQ=255)
                                                                                                 cTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTg                                           <  3:15961/149‑1 (MQ=255)
                                                                                                 cTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTg                                           <  4:987/149‑1 (MQ=255)
                                                                                                  tACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCAt                                                  <  1:366857/141‑1 (MQ=255)
                                                                                                  tACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCAt                                                  >  2:366857/1‑141 (MQ=255)
                                                                                                  tACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTg                                           >  2:210200/1‑148 (MQ=255)
                                                                                                   aCCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTggg                                         >  2:206726/1‑149 (MQ=255)
                                                                                                    ccGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTg                                           <  1:210200/146‑1 (MQ=255)
                                                                                                     cGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGgt                                       >  2:93537/1‑149 (MQ=255)
                                                                                                      gcacgggcacggGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGgtt                                      >  7:307965/1‑149 (MQ=255)
                                                                                                       cacgggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGcc                                                                                              >  1:329495/1‑92 (MQ=255)
                                                                                                       cacgggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGGTGCAGTGCGGGCCGTGTACATGGcc                                                                                              <  2:329495/92‑1 (MQ=255)
                                                                                                        acgggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGgttgt                                    <  7:350236/149‑1 (MQ=255)
                                                                                                         cgggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGgttgtt                                   <  8:294397/149‑1 (MQ=255)
                                                                                                          gggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGttgttg                                  >  8:40170/1‑149 (MQ=255)
                                                                                                            gcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTggg                                <  3:6997/149‑1 (MQ=255)
                                                                                                                     gTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAg                       <  3:401021/149‑1 (MQ=255)
                                                                                                                      ttACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAgg                      <  4:117877/149‑1 (MQ=255)
                                                                                                                      gtACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAgg                      <  8:69189/148‑1 (MQ=255)
                                                                                                                        aCGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTTGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAggg                     <  6:399051/148‑1 (MQ=255)
                                                                                                                          ggCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCGGTGTAGATGGCCGTGGTCGTGGTGGTGATCATGGCCAGGCTTGGAGTCAGGGCCATGAACGAGGGGTTGTTGGGACATTGCAGGGt                    >  6:407686/1‑147 (MQ=255)
                                                                                                                           gCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGAACATGGCCGTGGTCGTGGTGGTGATCATCGCCATGGTTGCTGTCAGGGCCATGTACGAGGGGTAGTTGGGACATTGCAGGGTCAc                 >  3:343285/1‑149 (MQ=255)
                                                                                                                            gcAACGCCCTGAAGGACGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTggg                                         <  3:44548/123‑1 (MQ=255)
                                                                                                                            gcAACGCCCTGAAGGACGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGAAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTggg                                         >  4:44548/2‑124 (MQ=255)
                                                                                                                             cAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTc               <  7:182456/149‑1 (MQ=255)
                                                                                                                              aaCGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTcc              >  6:400249/1‑149 (MQ=255)
                                                                                                                                cGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATgg                                                                        <  3:341478/89‑1 (MQ=255)
                                                                                                                                cGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATgg                                                                        >  4:341478/1‑89 (MQ=255)
                                                                                                                                cGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCgg            <  5:105677/149‑1 (MQ=255)
                                                                                                                                   cctgaaGGACGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTAt         >  5:203230/6‑149 (MQ=255)
                                                                                                                                    cTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATg        <  7:40170/149‑1 (MQ=255)
                                                                                                                                         gggCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAAtc   <  2:180718/149‑1 (MQ=255)
                                                                                                                                         gggCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAAtc   <  1:206726/149‑1 (MQ=255)
                                                                                                                                          ggCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAAtcg  >  5:345709/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
TCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCG  >  NC_002947/1273314‑1273600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑