| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,275,087 | G→C | 18.0% | intergenic (‑284/+28) | PP_5462 ← / ← PP_5463 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,275,087 | 0 | G | C | 18.0% | 48.1 / 10.1 | 28 | intergenic (‑284/+28) | PP_5462/PP_5463 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/10); new base C (3/2); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01 | |||||||||||
AATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTC > NC_002947/1274944‑1275231 | aaTGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgg < 1:241053/149‑1 (MQ=255) tGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgggc < 5:53123/149‑1 (MQ=255) gCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCttt < 4:131386/149‑2 (MQ=255) gCATTTTGGTCTATCCGGCGGAGAGTGAATCTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCtt > 1:32969/1‑148 (MQ=255) ttttGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgg > 7:289311/1‑149 (MQ=255) ttCTATCCGGCGTAGAGTGAATGTGGTAGCCTGCTTCCCGGTTACGTCGGATCCAATAAGGTGCGGGAGAGCTGTAATGATGGGGGTTATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgggggg < 8:145257/147‑1 (MQ=255) gTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAggggtgg > 7:386681/1‑149 (MQ=255) cgTGAACGTCCAAGCCTGCTTCCCGGGTACGTCGGCTCCAATAAGCTGCGGGAGAGCGGGAATGATGCGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCg < 8:386681/148‑1 (MQ=255) cTGCTTCCCGCTTACGTCGGCTCCACTACGCGGCGGGAGAGCCGTAATGATGGGCGCGATCGTGGTAAAGGCGAAACGACAACGACCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTACCGTACTACGGTCGCTtc < 2:32969/149‑1 (MQ=255) gcagcccGGTGACGGCGCCTCCAATAAGGTGCGGGAGAGCGGGAATGATGGGTGTGCTGGTGGTCAAGGTGACAGGAGACTGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGTGGGCAGAAGGGCTATCGTACTACGGTCGCTtctc < 8:289311/145‑1 (MQ=255) gCTTCCCGCTTACCTCGGCTCCAATACCGTGCGGGAGAGCGGTAACGAGGGGGGGCATGGCGCAAAAGGGCAACGGAGAATGCGCCACGCCCCCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTtctc < 4:204440/149‑1 (MQ=255) ttCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCGTCTCGt > 4:205839/1‑149 (MQ=255) tCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgggc < 3:101866/103‑1 (MQ=255) tCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgggc > 4:101866/1‑103 (MQ=255) tCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAgg > 2:323192/1‑148 (MQ=255) tggtggTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACttt > 5:151343/1‑147 (MQ=255) tAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGAc > 7:216599/1‑149 (MQ=255) gTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCt > 8:61398/1‑149 (MQ=255) gTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCt > 2:392433/1‑149 (MQ=255) gagaATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGAt > 7:268151/1‑148 (MQ=255) attaccAAACCCCGCCGATGGGCGGGCTTCAGGGGGGGGCATAATGGCTTTCGTACTACGGTCGCTTCTCGTCCACCGAAATGTAGGAATCACCCGCCCGCACTCCTCATCATGAATCTCACTTTCTGGTGACTGCCTTTGGATAAGtt < 7:61398/144‑1 (MQ=255) atgccgaaaGCCCGCCGATGGGCTGGCTAGAGGGGGGGGCTGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCAACGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGtt < 3:369703/143‑1 (MQ=255) ccctgaaGCCCGCCCATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGt > 3:379928/6‑149 (MQ=255) gCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCGTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTAt > 7:99107/1‑149 (MQ=255) gCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAg > 4:244834/1‑124 (MQ=255) ctgaaGCCCGCCCATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTgg > 4:193449/4‑138 (MQ=255) ctgaaGCCCGCCCATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTgg < 3:193449/135‑1 (MQ=255) ccgcccATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCt > 3:365467/1‑149 (MQ=255) cgATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTc < 6:151343/149‑1 (MQ=255) cgATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTc < 1:392433/149‑1 (MQ=255) aTGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCttt < 6:201660/123‑1 (MQ=255) aTGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCgtt > 5:201660/1‑123 (MQ=255) | AATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTC > NC_002947/1274944‑1275231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |