Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,288,148	C→T	5.9%	P511S (CCC→TCC)	PP_1125 →	putative Helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,288,148	0	C	T	5.9%	208.5 / 2.1	67	P511S (CCC→TCC)	PP_1125	putative Helicase
Reads supporting (aligned to +/- strand):  ref base C (31/32);  new base T (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GCCGCCGATATGCTGCGGCGCAGCCTGTGGAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACATCGTCGAAGATGCCCGTGGCGCGCTGGTGTTGTTCTC  >  NC_002947/1288007‑1288296                                                                                                                                               |                                                                                                                                                     gccgccGATATGCTGCGGCGCAGCCTGTGGAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGc                                                                                                                                                 <  5:42437/147‑1 (MQ=255) gccgccGATATGCTGCGGCGCAGCCTGTGGAGCGTGGCCCACGGCGCGCGGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGccc                                                                                                                                               <  5:186649/149‑1 (MQ=255)     ccgATATGCTGCGGCGCAGCCTGTGGAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTc                                                                                                                                           <  5:421884/149‑1 (MQ=255)            gctgcGGCGCAGCCTGTGGAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATg                                                                                                                                    >  6:50307/1‑149 (MQ=255)                  gcgcAGCCTGTGGAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATg                                                                                                                              >  8:151269/1‑149 (MQ=255)                    gcAGCCTGTGGAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGAACGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGcc                                                                                                                            >  6:221075/1‑149 (MQ=255)                      aGCCGGTGTAGCGGGGCCCACGGGGCGTGGGGCACCTCGCGTACATTCACCGCGCTGGGAAC‑GTTCCACCGTTTTCCAAGGTGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCgg                                                                                                                          <  1:333482/149‑1 (MQ=255)                          tgtgGAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTg                                                                                                                      >  8:166199/1‑149 (MQ=255)                              gAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGccaggggg                                                                                                                  >  1:145913/1‑143 (MQ=255)                              gAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGc                                                                                                                  <  1:312434/149‑1 (MQ=255)                               aGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCg                                                                                                                 <  2:353407/149‑1 (MQ=255)                                gCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCgg                                                                                                                <  6:204296/149‑1 (MQ=255)                                      cccACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTcccc                                                                                                          <  2:375182/149‑1 (MQ=255)                                       ccACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGTCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTcccc                                                                                                          >  6:42778/1‑148 (MQ=255)                                       ccACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGTCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCg                                                                                                         >  8:8419/1‑149 (MQ=255)                                                  tGGTGACCTCGGCGACGTTGACCGCGCTGGGCAATGTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGc                                                                                                                                       >  1:61187/1‑109 (MQ=255)                                                  tGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGcc                                                                                              >  5:322535/1‑149 (MQ=255)                                                   ggTGACCTCGGCGACATTGACCGCGCTGAGCAA‑GTTCGACCGTTTTCGCATGCGTTCGTGGTTGCCGCGCGACGCAGTCACCTGCTTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGGCCCCGACCTGCAGGCCg                                                                                             <  8:161400/149‑1 (MQ=255)                                                     tGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGAc                                                                                           <  5:391926/149‑1 (MQ=255)                                                        ccTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCa                                                                                        >  7:312650/1‑149 (MQ=255)                                                         cTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGTGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCg                                                                                                                 >  3:200892/1‑123 (MQ=255)                                                         cTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGTGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCg                                                                                                                 <  4:200892/123‑1 (MQ=255)                                                         cTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCAc                                                                                       >  6:56316/1‑149 (MQ=255)                                                          tCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCgg                                                                                                                >  2:72144/1‑123 (MQ=255)                                                          tCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCgg                                                                                                                <  1:72144/123‑1 (MQ=255)                                                          tCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCAcg                                                                                      >  1:237457/1‑149 (MQ=255)                                                          tCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCAcg                                                                                      >  1:243675/1‑149 (MQ=255)                                                          tCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCAcg                                                                                      >  8:156419/1‑149 (MQ=255)                                                                    tGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATgcag                                                                              >  5:198057/1‑147 (MQ=255)                                                                          cgcTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCCCCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCAGGCCTGTTGCGGGTCCCCGACCTGCAGGCGGCACCACGCGATGCAGCAGCGca                                                                       >  8:378393/1‑148 (MQ=255)                                                                           gcTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCg                                                                                                         >  1:309121/1‑113 (MQ=255)                                                                           gcTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCg                                                                                                         <  2:309121/113‑1 (MQ=255)                                                                               ggCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACcgc                                                                 >  2:123892/1‑149 (MQ=255)                                                                                 cAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCgcgg                                                               <  8:338170/149‑1 (MQ=255)                                                                                 cAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCgcgg                                                               >  8:339874/1‑149 (MQ=255)                                                                                     gTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGTCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGa                                                            <  7:8419/149‑1 (MQ=255)                                                                                     gTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGTCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGa                                                            <  5:42778/149‑1 (MQ=255)                                                                                     gTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCa                                                                                                  <  3:266914/111‑1 (MQ=255)                                                                                     gTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCa                                                                                                  >  4:266914/1‑111 (MQ=255)                                                                                     gTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGa                                                            <  2:237457/149‑1 (MQ=255)                                                                                     gTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGa                                                            <  2:243675/149‑1 (MQ=255)                                                                                         gACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGatcat                                                        >  4:56163/1‑149 (MQ=255)                                                                                         gACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGatca                                                         <  7:166199/148‑1 (MQ=255)                                                                                          aCCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGatcatc                                                       <  7:151269/149‑1 (MQ=255)                                                                                          aCCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGatcatc                                                       <  7:71303/149‑1 (MQ=255)                                                                                          aCCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGatcatc                                                       <  7:156419/149‑1 (MQ=255)                                                                                              ttttCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCcgc                                                    >  3:268836/1‑148 (MQ=255)                                                                                                    cATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGc                                             <  4:82250/149‑1 (MQ=255)                                                                                                     aTGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGcc                                            <  3:56163/149‑1 (MQ=255)                                                                                                     aTGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGcc                                            <  8:312650/149‑1 (MQ=255)                                                                                                     aTGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGcc                                            >  5:39772/1‑149 (MQ=255)                                                                                                         gTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGc                                                                                                                  <  4:178699/75‑1 (MQ=255)                                                                                                         gTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGc                                                                                                                  >  3:178699/1‑75 (MQ=255)                                                                                                           tCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGcc                                            >  7:248003/1‑143 (MQ=255)                                                                                                           tCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGcc                                            <  8:248003/143‑1 (MQ=255)                                                                                                               ggTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGCCCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACAtcgtc                                  >  2:343759/1‑149 (MQ=255)                                                                                                                     cgcgcgACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTg                                              >  8:354689/1‑131 (MQ=255)                                                                                                                     cgcgcgACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTg                                              <  7:354689/131‑1 (MQ=255)                                                                                                                      gcgcgACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAgcgc                                                                        >  1:48395/1‑104 (MQ=255)                                                                                                                      gcgcgACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAgcgc                                                                        <  2:48395/104‑1 (MQ=255)                                                                                                                             gCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACAtc                                     <  1:189781/132‑1 (MQ=255)                                                                                                                             gCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACAtc                                     >  2:189781/1‑132 (MQ=255)                                                                                                                                gTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACATCGTCGAAGATGCCCGTGgcgc                 >  3:152995/1‑149 (MQ=255)                                                                                                                                        cGTGGTGCCCAGCCCGTTCGTTGATGGAGATGCCTGGCTGTTGCGTTTCCCCGACCTGCAGGCCGACCCATGCTTTGCAGGAGCGCACTCCGCGGGGATCTTCCGGGTACTGCCTAACATGGTCGAAGATGCACGTGGCGGGATGgtg          <  6:322535/148‑1 (MQ=255)                                                                                                                                         gtggtgCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACATCGTCGAAGATGCCCGTGGCGCGCTGGtgtt        >  7:123395/1‑149 (MQ=255)                                                                                                                                          tggtgCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACATCGTCGAAGATGCCCGTGGCGCGCTGGtgttg       <  8:123395/149‑1 (MQ=255)                                                                                                                                          tggtgCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACATCGTCGAAGATGCCCGTGGCGCGCTGGtgttg       <  7:339874/149‑1 (MQ=255)                                                                                                                                               cccAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACATCGTCGAAGATGCCCGTGGCGCGCTGGTGTTGTtctc  <  5:221075/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     GCCGCCGATATGCTGCGGCGCAGCCTGTGGAGCGTGGCCCACGGCGCGCTGGTGACCTCGGCGACATTGACCGCGCTGGGCAA‑GTTCGACCGTTTTCGCATGCGTTCGGGGTTGCCGCGCGACGCAGTCACCTGCGTGGTGCCCAGCCCGTTCGTGCATGGCGATGCCGGCCTGTTGCGGGTCCCCGACCTGCAGGCCGACCCACGCGATGCAGCAGCGCACACCGCGGCGATCATCCGCGAACTGCCGAACATCGTCGAAGATGCCCGTGGCGCGCTGGTGTTGTTCTC  >  NC_002947/1288007‑1288296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑