Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,317,338	G→C	6.4%	Q252H (CAG→CAC)	PP_1150 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,317,338	0	G	C	6.4%	256.7 / 6.2	78	Q252H (CAG→CAC)	PP_1150	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (33/40);  new base C (3/2);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

AGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAGCAAATCA  >  NC_002947/1317192‑1317480                                                                                                                                                    |                                                                                                                                                aGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTg                                                                                                                                               <  3:3398/149‑1 (MQ=255)   ccTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTggc                                                                                                                                             >  7:137205/1‑149 (MQ=255)          cgcATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACt                                                                                                                                      <  7:312387/149‑1 (MQ=255)             aTCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGa                                                                                                                                   >  3:160433/1‑149 (MQ=255)                gTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGa                                                                                                                                <  1:343151/149‑1 (MQ=255)                gTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGa                                                                                                                                <  1:46825/149‑1 (MQ=255)                   gCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAgg                                                                                                                              <  3:110197/148‑1 (MQ=255)                    ccATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGt                                                                                                                            <  7:134863/149‑1 (MQ=255)                      aTCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGt                                                                                                                            >  7:123978/1‑147 (MQ=255)                      aTCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGa                                                                                                                          <  1:23456/149‑1 (MQ=255)                      aTCGACAGCCAACGTGAACATGCCTGGGTCGAGGGCAAGCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGa                                                                                                                          <  4:120586/149‑1 (MQ=255)                          aCAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAgg                                                                                                                        >  2:318173/1‑147 (MQ=255)                             gCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTagag                                                                                                                   <  4:160433/149‑1 (MQ=255)                              ccAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTagag                                                                                                                   >  6:316501/1‑148 (MQ=255)                              ccAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTagag                                                                                                                   >  8:139647/1‑148 (MQ=255)                              ccAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGc                                                                                                                  <  8:137205/149‑1 (MQ=255)                               cAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGc                                                                                                                  >  6:279889/1‑148 (MQ=255)                               cAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGc                                                                                                                  <  5:279889/148‑1 (MQ=255)                               cAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCa                                                                                                                 >  6:12104/1‑149 (MQ=255)                               cAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCACTGGCGGCAACTGGATGAGGGTGAGGTAGAGCa                                                                                                                 >  1:369718/1‑149 (MQ=255)                                aaCGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGa                                                                                                                          >  3:235006/1‑139 (MQ=255)                                aaCGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGa                                                                                                                          <  4:235006/139‑1 (MQ=255)                                   gTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTg                                                                                                            <  8:123978/149‑1 (MQ=255)                                      aaCATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGAc                                                                                                          <  6:345910/148‑1 (MQ=255)                                      aaCATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACg                                                                                                         >  8:174210/1‑149 (MQ=255)                                          tGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGAc                                                                                                          >  6:222797/1‑144 (MQ=255)                                          tGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGAc                                                                                                          <  5:222797/144‑1 (MQ=255)                                           gCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAgg                                                                                                                        >  6:285781/1‑130 (MQ=255)                                           gCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAgg                                                                                                                        <  5:285781/130‑1 (MQ=255)                                           gCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTg                                                                                                    >  7:18897/1‑149 (MQ=255)                                            ccTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTTCCGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTg                                                                                                    <  1:318173/149‑1 (MQ=255)                                            ccTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTgg                                                                                                   <  5:316501/149‑1 (MQ=255)                                             cTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGTTGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGGGAGGTAGAGCA‑TCTGACGCCCTGGt                                                                                                  <  5:263309/149‑1 (MQ=255)                                             cTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGc                                                                                                                  <  6:320043/134‑1 (MQ=255)                                             cTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGc                                                                                                                  >  5:320043/1‑134 (MQ=255)                                             cTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGt                                                                                                  >  1:167813/1‑149 (MQ=255)                                                   cGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAg                                                                                             <  5:12104/148‑1 (MQ=255)                                                      gggCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTggcg                                                                                                                                            <  4:30518/99‑1 (MQ=255)                                                      gggCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTggcg                                                                                                                                            >  3:30518/1‑99 (MQ=255)                                                         cAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTgc                                                                                      <  7:94076/149‑1 (MQ=255)                                                           aTCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTgcgc                                                                                    <  8:18897/149‑1 (MQ=255)                                                               cgGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACgc                                                                                <  6:19288/149‑1 (MQ=255)                                                                     ccAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTgg                                                                          >  8:351922/1‑149 (MQ=255)                                                                       atcgttcccgtgccTTTCGTGGCCTTAGCCACAAACTGATGGAGCTGTTGCGTATTTACCGTT‑CGGTGCGCCGGCAGTGGCGGGAACTGGATGAGGGGGAGGTAGAGCA‑TCTGAGGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGcc                                                                        <  5:78908/147‑1 (MQ=255)                                                                               cgtgccATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCACTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGa                                                                <  2:369718/149‑1 (MQ=255)                                                                                gtgccATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGAt                                                               <  7:174210/149‑1 (MQ=255)                                                                                gtgccATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGAt                                                               >  8:388040/1‑149 (MQ=255)                                                                                      ttCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATcagccc                                                         <  6:70694/149‑1 (MQ=255)                                                                                          tGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATcagcccagcc                                                     >  2:141364/1‑149 (MQ=255)                                                                                          tGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATcagcccagcc                                                     >  8:201029/1‑149 (MQ=255)                                                                                              cTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGGT‑CGGTGCGGCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGa                                                                                                           <  4:22216/91‑1 (MQ=255)                                                                                              cTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGAT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGa                                                                                                           >  3:22216/1‑91 (MQ=255)                                                                                                  gCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCtgtt                                              <  2:167813/148‑1 (MQ=255)                                                                                                    cAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTgttgtt                                           >  2:98206/1‑149 (MQ=255)                                                                                                     aGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGttgttg                                          >  4:125265/1‑149 (MQ=255)                                                                                                     aGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGttgttg                                          >  5:235217/1‑149 (MQ=255)                                                                                                      gAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGc                                         >  4:56476/1‑149 (MQ=255)                                                                                                      gAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCACCCTGCTGTTGTTGc                                         <  7:351922/149‑1 (MQ=255)                                                                                                        aaCTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAAcc                                                                   >  2:74812/1‑121 (MQ=255)                                                                                                        aaCTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAAcc                                                                   <  1:74812/121‑1 (MQ=255)                                                                                                                   cTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTg                            <  7:201029/149‑1 (MQ=255)                                                                                                                      ttGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGa                         >  1:81608/1‑149 (MQ=255)                                                                                                                         cGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCtgttgt                                            >  4:71182/1‑127 (MQ=255)                                                                                                                         cGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCtgttgt                                            <  3:71182/127‑1 (MQ=255)                                                                                                                          gTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATgcc                     >  7:213630/1‑149 (MQ=255)                                                                                                                            aTTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATgccgc                   >  6:68795/1‑149 (MQ=255)                                                                                                                             tttCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATgccgc                   <  6:235217/148‑1 (MQ=255)                                                                                                                                cccGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCATTATGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCCGCCTGCTGTTGTTGCGTCAGCGAATCTGGGTTGCCGCCCa                >  1:313648/1‑149 (MQ=255)                                                                                                                                cccGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCa                <  3:56476/148‑1 (MQ=255)                                                                                                                                cccGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCAc               >  7:131709/1‑149 (MQ=255)                                                                                                                                       ccagttgccgccACTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAg         >  3:147466/7‑149 (MQ=255)                                                                                                                                       cGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGa          <  5:68795/148‑1 (MQ=255)                                                                                                                                       cGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAg         >  2:183668/1‑149 (MQ=255)                                                                                                                                        ggTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAGc        <  8:213630/149‑1 (MQ=255)                                                                                                                                           ttgccgccACTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAGCaaa     >  6:82177/3‑149 (MQ=255)                                                                                                                                            cgccgcCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAGCAAAt    <  7:388040/149‑1 (MQ=255)                                                                                                                                              ccgccAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAGCAAATCa  <  2:81608/149‑1 (MQ=255)                                                                                                                                              ccgccACTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAGCAAATCa  <  5:82177/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                AGCCTGGGGCGCATCGTTGCCATCGACAGCCAACGTGAACATGCCTGGTTCGAGGGCAATCGCGGGCGCCAACGTGCCCGTGCCATTCGTGGCCTTAGCCAGAAACTGATGGTGCTGTTGCGTATTTCCCGTT‑CGGTGCGCCGCCAGTGGCGGCAACTGGATGAGGGTGAGGTAGAGCA‑TCTGACGCCCTGGTTGCAGGAAGTGCGCACGCTGCTGGCCCAACCCGATCAGCCCAGCCTGCTGTTGTTGCGTCAGCGAATCTGGGATGCCGCCCACGATGAGCAAATCA  >  NC_002947/1317192‑1317480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑