Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,350,502	T→A	5.7%	K11I (AAA→ATA) ‡	PP_1176 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,350,502	0	T	A	5.7%	217.0 / 2.5	70	K11I (AAA→ATA) ‡	PP_1176	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/35);  new base A (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

CCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTC  >  NC_002947/1350359‑1350648                                                                                                                                                |                                                                                                                                                     ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                   >  6:412415/1‑147 (MQ=255) ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                   >  6:372870/1‑147 (MQ=255)        tGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                          >  6:113958/1‑149 (MQ=255)          aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCGATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         >  3:246704/1‑148 (MQ=255)          aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         <  4:330950/148‑1 (MQ=255)          aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         >  8:266837/1‑148 (MQ=255)          aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttt                                                                                                                                          <  1:4874/147‑3 (MQ=255)           cGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGa                                                                                                                                       <  5:68337/149‑1 (MQ=255)              gaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGc                                                                                                                                    <  7:266837/149‑1 (MQ=255)                aCGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTc                                                                                                                                  <  5:372870/149‑1 (MQ=255)                 cGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCa                                                                                                                                 >  7:274284/1‑149 (MQ=255)                    gATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑Gcc                                                                                                                             <  2:229197/149‑1 (MQ=255)                      ttCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCGATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCg                                                                                                                           <  4:246704/149‑1 (MQ=255)                        ccGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGt                                                                                                                         >  6:188217/1‑149 (MQ=255)                          gCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGtc                                                                                                                        >  8:129149/1‑148 (MQ=255)                              tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGtcaatc                                                                                                                    >  3:123302/1‑148 (MQ=255)                              tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGtcaat                                                                                                                     <  5:129508/147‑1 (MQ=255)                                 gAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCaatcaatc                                                                                                                >  6:166255/1‑149 (MQ=255)                                    cGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCa                                                                                                             <  8:276473/149‑1 (MQ=255)                                     gTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAg                                                                                                            <  4:301300/149‑1 (MQ=255)                                     gTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAg                                                                                                            >  2:393604/1‑149 (MQ=255)                                     gTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAg                                                                                                            <  2:258932/149‑1 (MQ=255)                                      ttCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGc                                                                                                           <  4:123302/149‑1 (MQ=255)                                           aaGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTc                                                                                                         <  5:188217/146‑1 (MQ=255)                                                   gAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAGTCAATCCCAGCTAAC‑CGCCCCAca                                                                                             >  8:285013/1‑148 (MQ=255)                                                    aaaaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCAccc                                                                                            >  3:134151/1‑149 (MQ=255)                                                     aaaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCAccc                                                                                            <  4:134151/148‑1 (MQ=255)                                                      aaCGTTATCGCGATGTCCAAAAAGAGTCAAGGTCGGCAGCAGTAGGAAAATTCGTTTTCTGCTTATGCCAGGGAATAAACAGTCTGGCATTTGGCAGCCTTGTTGATGCTCT‑GCCCGGTCAATCAATCCCATCTCAA‑CGCCCCaggcgt                                                                                          <  8:372055/149‑6 (MQ=255)                                                         gTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCt                                                                                        >  1:304024/1‑148 (MQ=255)                                                          ttATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTg                                                                                       >  3:296182/1‑148 (MQ=255)                                                               gcgATGTCCAAAAAGAGGCACTGTAGGCAGGCGTAGGTAACGTCCTGTTCTGCTTCTGCCAGGCAATATAATGGCTGCTATTTGGCAGCCTTGTTTATTATGA‑GCCCGTTCAATCAATCACTGCTCAA‑GGCCATACCCGTCTGATACTc                                                                                 <  4:267924/149‑1 (MQ=255)                                                               gcgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACt                                                                                  <  5:412415/148‑1 (MQ=255)                                                                       cAAAAACATGCAAGGTCGGCAGCCTTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCATCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCacac                                                                         <  7:285013/149‑1 (MQ=255)                                                                       cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCacac                                                                         >  6:236582/1‑149 (MQ=255)                                                                       cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCacac                                                                         <  1:393604/149‑1 (MQ=255)                                                                       cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCacac                                                                         <  5:166255/149‑1 (MQ=255)                                                                        aaaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACAcg                                                                        >  1:392796/1‑149 (MQ=255)                                                                         aaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTAA‑GCCCGGTCAATAAATCCCAGATCAA‑CGCCCCACCCGTCTGATACTCGAACACAcgc                                                                       >  2:369183/1‑149 (MQ=255)                                                                          aaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACAcgcg                                                                      >  7:129307/1‑149 (MQ=255)                                                                            aCATGCAAGGTCGGGATGCGTACGAAACGTCCTGTTCTGCTTCTGCCATGCAATAAAAACGCTGCCATTTGGCATCCTTGTTTATTCTTATCCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGt                                                                     <  1:106852/149‑1 (MQ=255)                                                                            aCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtc                                                                    <  4:296182/149‑1 (MQ=255)                                                                              aGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtct                                                                   >  7:347495/1‑148 (MQ=255)                                                                               ggCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtctgg                                                                 <  5:113958/149‑3 (MQ=255)                                                                                gCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGt                                                                                                                         >  8:117667/1‑93 (MQ=255)                                                                                gCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGt                                                                                                                         <  7:117667/93‑1 (MQ=255)                                                                                   aGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaag                                                              >  3:41332/1‑148 (MQ=255)                                                                                          cAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGttctt                                                      <  7:129149/149‑1 (MQ=255)                                                                                                  ggAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCcttct                                              >  5:144028/1‑149 (MQ=255)                                                                                                  ggAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCcttc                                               <  1:369183/148‑1 (MQ=255)                                                                                                   gAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCcttctt                                             <  2:304024/149‑1 (MQ=255)                                                                                                     aaCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCcttc                                               >  3:269356/1‑145 (MQ=255)                                                                                                     aaCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCcttc                                               <  4:269356/145‑1 (MQ=255)                                                                                                       cGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGACTCAAAGAAGTTCTTCTCCttcttc                                            >  6:311671/1‑146 (MQ=255)                                                                                                         tCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTc                                       >  3:67107/1‑149 (MQ=255)                                                                                                         tCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTc                                       <  4:279421/149‑1 (MQ=255)                                                                                                          ccTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGTCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGGCTGATACTCGATCACACGCGTCTCAAAGAAGGTCTTCTCCTTCTTCAAGTc                                       >  4:19165/1‑148 (MQ=255)                                                                                                                    ttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGc                            >  3:222580/1‑149 (MQ=255)                                                                                                                      ctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGc                            <  4:41332/147‑1 (MQ=255)                                                                                                                        gcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCAt                        >  8:134857/1‑149 (MQ=255)                                                                                                                        gcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTACGTCGTCAAGTCCAGGATAGCGCtaa                         >  8:327806/1‑146 (MQ=255)                                                                                                                         ccAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCAt                                    <  4:176829/136‑1 (MQ=255)                                                                                                                         ccAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCAt                                    >  3:176829/1‑136 (MQ=255)                                                                                                                             gCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATg                   <  7:134857/149‑1 (MQ=255)                                                                                                                              cAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                  <  5:236582/149‑1 (MQ=255)                                                                                                                                   acaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATggg                 >  4:286683/3‑145 (MQ=255)                                                                                                                                   acaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATggg                 <  3:286683/143‑1 (MQ=255)                                                                                                                                    aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg            <  2:392796/149‑1 (MQ=255)                                                                                                                                      aGGCTGCCAAATGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAAGCGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACggg           >  1:318411/1‑149 (MQ=255)                                                                                                                                            ccATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTa     <  4:222580/148‑1 (MQ=255)                                                                                                                                              aTTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTc  <  8:129307/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     CCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCA‑GCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTC  >  NC_002947/1350359‑1350648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑