Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,373,817 A→T 7.0% L46Q (CTG→CAG)  PP_1199 ← toxin‑antitoxin system HigB‑like toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,373,8170AT 7.0% 251.2 / 5.1 82L46Q (CTG→CAG) PP_1199toxin‑antitoxin system HigB‑like toxin
Reads supporting (aligned to +/- strand):  ref base A (39/37);  new base T (3/3);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGTATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGCTG  >  NC_002947/1373670‑1373964
                                                                                                                                                   |                                                                                                                                                   
aaGGTATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCa                                                                                                                                                     <  2:409441/148‑1 (MQ=255)
 aGGTATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGATGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAg                                                                                                                                                    >  7:341217/1‑148 (MQ=255)
  ggTATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAg                                                                                                                                                    >  3:89735/1‑147 (MQ=255)
  ggTATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGga                                                                                                                                                  <  5:164629/149‑1 (MQ=255)
    tATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTGGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGgaga                                                                                                                                                <  2:275039/149‑1 (MQ=255)
         aCGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTCTCTCCTGGAGGAAAcc                                                                                                                                           >  2:44164/1‑149 (MQ=255)
             tGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTCTCTCCTGGAGGAAACCGGcg                                                                                                                                       <  1:44164/149‑2 (MQ=255)
              gCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCtt                                                                                                                                      >  6:61373/1‑149 (MQ=255)
                    gCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGa                                                                                                                                 <  6:277253/148‑1 (MQ=255)
                      gATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTCTCTCCTGGAGGAAACCGGc                                                                                                                                        >  8:224587/1‑139 (MQ=255)
                      gATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTCTCTCCTGGAGGAAACCGGc                                                                                                                                        <  7:224587/139‑1 (MQ=255)
                      gATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCCCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATg                                                                                                                               >  2:206633/1‑148 (MQ=255)
                           aTCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCGTCGGGATGCTCGGc                                                                                                                         >  4:225225/1‑149 (MQ=255)
                                aaTCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGa                                                                                                                     >  2:97549/1‑148 (MQ=255)
                                  tCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGTCAGATAg                                                                                                                  <  5:239116/149‑1 (MQ=255)
                                    aTACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAgg                                                                                                                 <  1:165230/148‑1 (MQ=255)
                                     tACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAAcc                                                                                                                                           <  8:318850/121‑1 (MQ=255)
                                     tACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAAcc                                                                                                                                           >  7:318850/1‑121 (MQ=255)
                                      aCGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCt                                                                                                              >  2:230479/1‑149 (MQ=255)
                                                tggGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCg                                                                                                    <  4:89735/149‑1 (MQ=255)
                                                tggGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCg                                                                                                    <  5:61373/149‑1 (MQ=255)
                                                tggGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCg                                                                                                    >  6:353430/1‑149 (MQ=255)
                                                     tGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTAtt                                                                                               >  3:274056/1‑149 (MQ=255)
                                                       cAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTAttct                                                                                             <  4:274056/149‑1 (MQ=255)
                                                                 gtagtaGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCa                                                                                   <  3:205304/149‑1 (MQ=255)
                                                                    gtagATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGt                                                                                <  7:122109/149‑1 (MQ=255)
                                                                          cACTCGTACACCCCCACTCTTGCCCATGCCCTAGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGCTTTCCTCAAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGTTAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAggagatcat                                                                          >  1:331398/1‑142 (MQ=255)
                                                                            cTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCCGTATTCTTCATCATCCAGGTGCTCCTTg                                                                        <  1:230479/149‑1 (MQ=255)
                                                                              cGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCtt                                                                         >  3:25577/1‑146 (MQ=255)
                                                                                   acCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTcc                                                                           >  2:280892/1‑139 (MQ=255)
                                                                                   acCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTcc                                                                           <  1:280892/139‑1 (MQ=255)
                                                                                   acCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCt                                                                          <  7:179049/140‑1 (MQ=255)
                                                                                   acCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCt                                                                          >  8:179049/1‑140 (MQ=255)
                                                                                    cccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCTGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGa                                                                <  5:362700/149‑1 (MQ=255)
                                                                                    cccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTtcatca                                                                                       >  4:50093/1‑126 (MQ=255)
                                                                                    cccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTtcatca                                                                                       <  3:50093/126‑1 (MQ=255)
                                                                                     ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCtt                                                                         >  4:200501/1‑139 (MQ=255)
                                                                                     ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCtt                                                                         >  2:50448/1‑139 (MQ=255)
                                                                                     ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCggtg                                                               >  5:364874/1‑147 (MQ=255)
                                                                                     ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCg                                                                  >  3:37865/1‑146 (MQ=255)
                                                                                     ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCg                                                                  <  4:37865/146‑1 (MQ=255)
                                                                                       ccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGt                                                             >  4:21534/1‑149 (MQ=255)
                                                                                       ccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGt                                                             >  3:133620/1‑149 (MQ=255)
                                                                                        cACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTg                                                            <  4:25577/149‑1 (MQ=255)
                                                                                         aCTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTg                                                            >  5:213590/1‑148 (MQ=255)
                                                                                           tctTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGt                                                             <  4:173744/145‑1 (MQ=255)
                                                                                           tctTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGt                                                             >  3:173744/1‑145 (MQ=255)
                                                                                           tctTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGaa                                                          >  8:39035/1‑148 (MQ=255)
                                                                                             ttgcccttgcccTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGTGGCGAATAc                                                       >  8:372342/1‑149 (MQ=255)
                                                                                               gcccttgcccttGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTAttcttc                                                                                           <  2:384189/111‑1 (MQ=255)
                                                                                               gcccttgcccttGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTAttcttc                                                                                           >  1:384189/1‑111 (MQ=255)
                                                                                                cccttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTg                                                     <  2:247609/148‑1 (MQ=255)
                                                                                                 ccttgccctttGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTggg                                                   <  1:30211/149‑1 (MQ=255)
                                                                                                 ccttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTggg                                                   <  1:97549/149‑1 (MQ=255)
                                                                                                 ccttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTggg                                                   <  3:200501/149‑1 (MQ=255)
                                                                                                 ccttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTggg                                                   >  2:148088/1‑149 (MQ=255)
                                                                                                  cttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTgggg                                                  >  7:314387/1‑149 (MQ=255)
                                                                                                  cttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTgggg                                                  >  7:213103/1‑149 (MQ=255)
                                                                                                       ccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGtctc                                              >  1:411418/1‑148 (MQ=255)
                                                                                                        cttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGa                                            <  8:213103/149‑1 (MQ=255)
                                                                                                           ggCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGAt                                           <  5:40819/147‑1 (MQ=255)
                                                                                                            gCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGaa                                        <  3:21534/149‑1 (MQ=255)
                                                                                                              tGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGa                                      <  1:206633/149‑1 (MQ=255)
                                                                                                                cccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAg                                                               >  5:82288/1‑122 (MQ=255)
                                                                                                                cccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAg                                                               <  6:82288/122‑1 (MQ=255)
                                                                                                                 ccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTga                                                                       <  4:239785/113‑1 (MQ=255)
                                                                                                                 ccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTga                                                                       >  3:239785/1‑113 (MQ=255)
                                                                                                                 ccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCa                                   <  6:213590/149‑1 (MQ=255)
                                                                                                                   aGCTGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGTTGCTCGGCCAGTTAGGCCTGCAAAGCCCGGTATTCTTCATCTTCGAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTGTCGTTGAAGATCATg                                 <  2:331398/149‑1 (MQ=255)
                                                                                                                       gATCTTTCTGAGGCCACCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGGCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGa                                         <  6:415938/137‑1 (MQ=255)
                                                                                                                       gATCTTTCTGAGGCCACCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGa                                         >  5:415938/1‑137 (MQ=255)
                                                                                                                          cTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTCCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGCAGGTGAATACTGGGGTCTCGAAGAAGATCATGCAGTca                           >  4:44091/1‑147 (MQ=255)
                                                                                                                            ttCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTg                        >  5:242767/1‑149 (MQ=255)
                                                                                                                              cTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGc                       <  7:39035/148‑1 (MQ=255)
                                                                                                                                   gccgccGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCg                 >  7:239423/1‑149 (MQ=255)
                                                                                                                                    ccgccgGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGt                <  1:148088/149‑1 (MQ=255)
                                                                                                                                           tttCCTCCTGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCTTCCATGTGCTCCTTGAGATCGGAGGTGAATACTGGGTTCTCGATGAAGTTCATGCAGTCCCTGCTATGCGTCAATGGc         <  3:44091/149‑1 (MQ=255)
                                                                                                                                             ctctccTGGAGGAAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCg        >  1:370404/3‑148 (MQ=255)
                                                                                                                                              cctccAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGt       <  8:239423/148‑1 (MQ=255)
                                                                                                                                               ctccAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAg     <  6:364874/149‑1 (MQ=255)
                                                                                                                                                tccAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGc    >  2:185692/1‑149 (MQ=255)
                                                                                                                                                tccAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGc    >  6:170238/1‑149 (MQ=255)
                                                                                                                                                  cAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGCTg  >  2:206009/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAGGTATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGCTG  >  NC_002947/1373670‑1373964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑