Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,373,822 A→G 6.3% S44S (TCT→TCC) ‡ PP_1199 ← toxin‑antitoxin system HigB‑like toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,373,8220AG 6.3% 231.1 / 3.6 78S44S (TCT→TCC) ‡PP_1199toxin‑antitoxin system HigB‑like toxin
Reads supporting (aligned to +/- strand):  ref base A (37/36);  new base G (3/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGCTG  >  NC_002947/1373674‑1373964
                                                                                                                                                    |                                                                                                                                              
tATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTGGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGgaga                                                                                                                                                <  2:275039/149‑1 (MQ=255)
     aCGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTCTCTCCTGGAGGAAAcc                                                                                                                                           >  2:44164/1‑149 (MQ=255)
         tGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTCTCTCCTGGAGGAAACCGGcg                                                                                                                                       <  1:44164/149‑2 (MQ=255)
          gCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCtt                                                                                                                                      >  6:61373/1‑149 (MQ=255)
                gCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGa                                                                                                                                 <  6:277253/148‑1 (MQ=255)
                  gATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTCTCTCCTGGAGGAAACCGGc                                                                                                                                        >  8:224587/1‑139 (MQ=255)
                  gATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTCTCTCCTGGAGGAAACCGGc                                                                                                                                        <  7:224587/139‑1 (MQ=255)
                  gATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCCCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATg                                                                                                                               >  2:206633/1‑148 (MQ=255)
                       aTCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCGTCGGGATGCTCGGc                                                                                                                         >  4:225225/1‑149 (MQ=255)
                            aaTCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGa                                                                                                                     >  2:97549/1‑148 (MQ=255)
                              tCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGTCAGATAg                                                                                                                  <  5:239116/149‑1 (MQ=255)
                                aTACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAgg                                                                                                                 <  1:165230/148‑1 (MQ=255)
                                 tACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAAcc                                                                                                                                           <  8:318850/121‑1 (MQ=255)
                                 tACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAAcc                                                                                                                                           >  7:318850/1‑121 (MQ=255)
                                  aCGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCt                                                                                                              >  2:230479/1‑149 (MQ=255)
                                            tggGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCg                                                                                                    <  4:89735/149‑1 (MQ=255)
                                            tggGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCg                                                                                                    <  5:61373/149‑1 (MQ=255)
                                            tggGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCg                                                                                                    >  6:353430/1‑149 (MQ=255)
                                                 tGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTAtt                                                                                               >  3:274056/1‑149 (MQ=255)
                                                   cAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTAttct                                                                                             <  4:274056/149‑1 (MQ=255)
                                                             gtagtaGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCa                                                                                   <  3:205304/149‑1 (MQ=255)
                                                                gtagATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGt                                                                                <  7:122109/149‑1 (MQ=255)
                                                                      cACTCGTACACCCCCACTCTTGCCCATGCCCTAGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGCTTTCCTCAAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGTTAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAggagatcat                                                                          >  1:331398/1‑142 (MQ=255)
                                                                        cTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCCGTATTCTTCATCATCCAGGTGCTCCTTg                                                                        <  1:230479/149‑1 (MQ=255)
                                                                          cGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCtt                                                                         >  3:25577/1‑146 (MQ=255)
                                                                               acCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTcc                                                                           <  1:280892/139‑1 (MQ=255)
                                                                               acCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTcc                                                                           >  2:280892/1‑139 (MQ=255)
                                                                               acCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCt                                                                          >  8:179049/1‑140 (MQ=255)
                                                                               acCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCt                                                                          <  7:179049/140‑1 (MQ=255)
                                                                                cccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCTGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGa                                                                <  5:362700/149‑1 (MQ=255)
                                                                                cccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTtcatca                                                                                       >  4:50093/1‑126 (MQ=255)
                                                                                cccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTtcatca                                                                                       <  3:50093/126‑1 (MQ=255)
                                                                                 ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCtt                                                                         >  2:50448/1‑139 (MQ=255)
                                                                                 ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCtt                                                                         >  4:200501/1‑139 (MQ=255)
                                                                                 ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCggtg                                                               >  5:364874/1‑147 (MQ=255)
                                                                                 ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCg                                                                  >  3:37865/1‑146 (MQ=255)
                                                                                 ccccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCg                                                                  <  4:37865/146‑1 (MQ=255)
                                                                                   ccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGt                                                             >  3:133620/1‑149 (MQ=255)
                                                                                   ccACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGt                                                             >  4:21534/1‑149 (MQ=255)
                                                                                    cACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTg                                                            <  4:25577/149‑1 (MQ=255)
                                                                                     aCTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTg                                                            >  5:213590/1‑148 (MQ=255)
                                                                                       tctTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGt                                                             <  4:173744/145‑1 (MQ=255)
                                                                                       tctTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGt                                                             >  3:173744/1‑145 (MQ=255)
                                                                                       tctTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGaa                                                          >  8:39035/1‑148 (MQ=255)
                                                                                         ttgcccttgcccTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGTGGCGAATAc                                                       >  8:372342/1‑149 (MQ=255)
                                                                                           gcccttgcccttGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTAttcttc                                                                                           <  2:384189/111‑1 (MQ=255)
                                                                                           gcccttgcccttGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTAttcttc                                                                                           >  1:384189/1‑111 (MQ=255)
                                                                                            cccttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTg                                                     <  2:247609/148‑1 (MQ=255)
                                                                                             ccttgccctttGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTggg                                                   <  1:30211/149‑1 (MQ=255)
                                                                                             ccttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTggg                                                   >  2:148088/1‑149 (MQ=255)
                                                                                             ccttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTggg                                                   <  1:97549/149‑1 (MQ=255)
                                                                                             ccttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTggg                                                   <  3:200501/149‑1 (MQ=255)
                                                                                              cttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTgggg                                                  >  7:314387/1‑149 (MQ=255)
                                                                                              cttgcccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTgggg                                                  >  7:213103/1‑149 (MQ=255)
                                                                                                   ccttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGtctc                                              >  1:411418/1‑148 (MQ=255)
                                                                                                    cttgGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGa                                            <  8:213103/149‑1 (MQ=255)
                                                                                                       ggCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGAt                                           <  5:40819/147‑1 (MQ=255)
                                                                                                        gCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGaa                                        <  3:21534/149‑1 (MQ=255)
                                                                                                          tGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGa                                      <  1:206633/149‑1 (MQ=255)
                                                                                                            cccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAg                                                               >  5:82288/1‑122 (MQ=255)
                                                                                                            cccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAg                                                               <  6:82288/122‑1 (MQ=255)
                                                                                                             ccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTga                                                                       <  4:239785/113‑1 (MQ=255)
                                                                                                             ccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTga                                                                       >  3:239785/1‑113 (MQ=255)
                                                                                                             ccAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCa                                   <  6:213590/149‑1 (MQ=255)
                                                                                                               aGCTGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGTTGCTCGGCCAGTTAGGCCTGCAAAGCCCGGTATTCTTCATCTTCGAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTGTCGTTGAAGATCATg                                 <  2:331398/149‑1 (MQ=255)
                                                                                                                   gATCTTTCTGAGGCCACCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGGCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGa                                         <  6:415938/137‑1 (MQ=255)
                                                                                                                   gATCTTTCTGAGGCCACCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGa                                         >  5:415938/1‑137 (MQ=255)
                                                                                                                      cTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTCCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGCAGGTGAATACTGGGGTCTCGAAGAAGATCATGCAGTca                           >  4:44091/1‑147 (MQ=255)
                                                                                                                        ttCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTg                        >  5:242767/1‑149 (MQ=255)
                                                                                                                          cTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGc                       <  7:39035/148‑1 (MQ=255)
                                                                                                                               gccgccGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCg                 >  7:239423/1‑149 (MQ=255)
                                                                                                                                ccgccgGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGt                <  1:148088/149‑1 (MQ=255)
                                                                                                                                       tttCCTCCTGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCTTCCATGTGCTCCTTGAGATCGGAGGTGAATACTGGGTTCTCGATGAAGTTCATGCAGTCCCTGCTATGCGTCAATGGc         <  3:44091/149‑1 (MQ=255)
                                                                                                                                         ctctccTGGAGGAAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCg        >  1:370404/3‑148 (MQ=255)
                                                                                                                                          cctccAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGt       <  8:239423/148‑1 (MQ=255)
                                                                                                                                           ctccAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAg     <  6:364874/149‑1 (MQ=255)
                                                                                                                                            tccAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGc    >  6:170238/1‑149 (MQ=255)
                                                                                                                                            tccAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGc    >  2:185692/1‑149 (MQ=255)
                                                                                                                                              cAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGCTg  >  2:206009/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TATCCACGATGCCCTTGCGATAGATCAGAATCATACGGATCTGGTGGGCTGCAGTGACGTGGTAGTAGATCACTCGTACACCCCCACTCTTGCCCTTGCCCTTGGCTGCCCAGCGGATCTTTCTGAGGCCGCCGGTTTCCTCCAGGAGAGAACCGGCTTCGGGATGCTCGGCCAGATAGGCCTGCAAAGCCCGGTATTCTTCATCATCCAGGTGCTCCTTGAGATCGGAGGTGAATACTGGGGTCTCGATGAAGATCATGCAGTCCCTGCTATGCGTCAATGGCGTAGCTG  >  NC_002947/1373674‑1373964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑