Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,414,811	A→T	6.3%	V28V (GTA→GTT)	dapA‑I →	4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,414,811	0	A	T	6.3%	194.1 / 2.5	63	V28V (GTA→GTT)	dapA‑I	4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base A (29/30);  new base T (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

GTGCAAGGTCGATGCCCCCAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGAGCGCGTCAAACGCAGCGCCAAACCGATTCCGGTCAT  >  NC_002947/1414669‑1414957                                                                                                                                               |                                                                                                                                                   gtgCAAGGTCGATGCCCCCAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACtt                                                                                                                                               <  5:87879/148‑1 (MQ=255) gtgCAAGGTCGATGCCCCCAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACt                                                                                                                                                <  7:345329/147‑1 (MQ=255)  tgCAAGGTCGATGCCCCCAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTcc                                                                                                                                             <  5:86093/149‑1 (MQ=255)  tgCAAGGTCGATGCCCCCAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTc                                                                                                                                              >  7:46225/1‑148 (MQ=255)      aGGTCGATGCCCCCAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGTGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCt                                                                                                                                         <  4:133967/149‑1 (MQ=255)               cccccAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACg                                                                                                                                 <  8:46225/148‑1 (MQ=255)                ccccAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCAATGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGAATTCCACCTGGAAAACGGc                                                                                                                               >  7:277915/1‑149 (MQ=255)                 cccAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTTGACTTCCACCTGGAAAACGGCa                                                                                                                              >  1:243861/1‑149 (MQ=255)                 cccAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTTGACTTCCACCTGGAAAACGGCa                                                                                                                              >  1:237678/1‑149 (MQ=255)                 cccAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCa                                                                                                                              <  2:396152/149‑1 (MQ=255)                  ccAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCAc                                                                                                                             >  1:257431/1‑149 (MQ=255)                             aCCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTTGACTTCCACCTGGAAAACGGCACCCATGCGAtc                                                                                                                   <  2:237678/148‑1 (MQ=255)                             aCCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTTGACTTCCACCTGGAAAACGGCACCCATGCGAtc                                                                                                                   <  2:243861/148‑1 (MQ=255)                                    tctTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGt                                                                                                           >  6:361958/1‑149 (MQ=255)                                      tttttCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTa                                                                                                                                                    <  7:345390/106‑1 (MQ=255)                                      tttttCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTa                                                                                                                                                    >  8:345390/1‑106 (MQ=255)                                      tttttCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCg                                                                                                         >  2:16665/1‑149 (MQ=255)                                       ttttCCGGAAGGAGCAGTTAGATGATTGCTGGTAGTATGGTGGCATTGGTCACACGCATGGATGCAGAAGGGCGTGTTGAATGGGGCAGCCTCGACAAAGTTGTAGACTTCCACCTGGTAATCGGCACCCATGCTATCGTCGCTGTCg                                                                                                         <  6:364949/148‑1 (MQ=255)                                             ggCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGcaccac                                                                                                  >  2:153217/1‑149 (MQ=255)                                               cAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCg                                                                                                <  1:153217/149‑1 (MQ=255)                                                  gAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGTCAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGt                                                                                              <  2:392082/148‑1 (MQ=255)                                                  gAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGTCAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGt                                                                                              >  1:392082/1‑148 (MQ=255)                                                   aGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGa                                                                                            <  2:257431/149‑1 (MQ=255)                                                              gATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGc                                                                                 >  5:112844/1‑149 (MQ=255)                                                              gATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGc                                                                                 >  4:21107/1‑149 (MQ=255)                                                                       cAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCgg                                                                                                        <  5:241656/117‑1 (MQ=255)                                                                       cAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCgg                                                                                                        >  6:241656/1‑117 (MQ=255)                                                                          tATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCgaag                                                                     <  1:16665/149‑1 (MQ=255)                                                                                 gCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAAcaca                                                               <  8:277915/148‑1 (MQ=255)                                                                                 gCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACAt                                                              >  3:378546/1‑149 (MQ=255)                                                                                  cATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATc                                                             >  1:301395/1‑149 (MQ=255)                                                                                   aTTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATcc                                                            >  7:187135/1‑149 (MQ=255)                                                                                   aTTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATcc                                                            <  6:270499/149‑1 (MQ=255)                                                                                   aTTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCACTGTCGGCACCACCGGTGAGTCGGCCACGCTg                                                                               >  6:199859/1‑130 (MQ=255)                                                                                   aTTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCACTGTCGGCACCACCGGTGAGTCGGCCACGCTg                                                                               <  5:199859/130‑1 (MQ=255)                                                                                      ggTCACACCCATGGATGCACAAGGGCGTGTTGACTGGTGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGTCACCACCTGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTg                                                          <  3:400875/148‑1 (MQ=255)                                                                                       gTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGACTCGACAAACTTGTAGACTTCCAACTGCAAAACGGAACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATACTg                                                          >  4:400875/1‑147 (MQ=255)                                                                                        tCACACCCATGGATGCACAAGGGCGTGTTTACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGt                                                                                              <  2:146226/110‑1 (MQ=255)                                                                                        tCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGt                                                                                              >  1:146226/1‑110 (MQ=255)                                                                                        tCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTggcc                                                       <  4:378546/149‑3 (MQ=255)                                                                                          acacCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGcc                                                                                     >  8:294998/1‑117 (MQ=255)                                                                                          acacCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGcc                                                                                     <  7:294998/117‑1 (MQ=255)                                                                                              ccATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCgaa                                                                      >  5:61521/1‑128 (MQ=255)                                                                                              ccATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCgaa                                                                      <  6:61521/128‑1 (MQ=255)                                                                                              ccATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCg                                                                        <  5:101629/126‑1 (MQ=255)                                                                                              ccATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCg                                                                        >  6:101629/1‑126 (MQ=255)                                                                                                aTGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCa                                               >  3:375300/1‑149 (MQ=255)                                                                                                aTGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCa                                               >  7:271287/1‑149 (MQ=255)                                                                                                         caAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTc                                        >  4:238503/1‑147 (MQ=255)                                                                                                         caAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGa                                      <  6:112844/149‑1 (MQ=255)                                                                                                                     gACTGGGGCAGCCTCGACAAAGTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGc                                                <  4:356870/127‑1 (MQ=255)                                                                                                                     gACTGGGGCAGCCTCGACAAACTAGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGc                                                >  3:356870/1‑127 (MQ=255)                                                                                                                          gggCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCgaagaa                                                                   >  4:82537/1‑103 (MQ=255)                                                                                                                          gggCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCgaagaa                                                                   <  3:82537/103‑1 (MQ=255)                                                                                                                          gggCAGCCTCGACAAACTAGTAGACTTCCACCTGGAAAACGGCACAAATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACCTCCTGGTCATCAAGCACGTGGTCCACCGCGTCAAACGCAgcg                     >  2:147254/1‑149 (MQ=255)                                                                                                                                 cTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTc                                        >  7:4578/1‑123 (MQ=255)                                                                                                                                 cTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTc                                        <  8:4578/123‑1 (MQ=255)                                                                                                                                  tCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGtcatc                                                    <  6:274498/110‑1 (MQ=255)                                                                                                                                  tCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGtcatc                                                    >  5:274498/1‑110 (MQ=255)                                                                                                                                    gACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGAGCGCGTCAAACGCAGCGCCAAACCGa            <  5:361958/148‑1 (MQ=255)                                                                                                                                    gACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGAGCGCGTCAAACGCAGCGCCAAACCGa            <  3:21107/148‑1 (MQ=255)                                                                                                                                    gACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGAGCGCGTCAAACGCAGCGCCAAACCGAt           >  2:179452/1‑149 (MQ=255)                                                                                                                                    gACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGAGCGCGTCAAACGCAGCGCCAAACCGAt           >  1:407384/1‑149 (MQ=255)                                                                                                                                        aaCTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGAGCGCGTCAAACGCAGCGCCAAACCGATTCCg       <  2:407384/149‑1 (MQ=255)                                                                                                                                              tAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGAGCGCGTCAAACGCAGCGCCAAACCGATTCCGGTCAt  <  3:238503/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   GTGCAAGGTCGATGCCCCCAGTACCATTACCGCTCTCTTTTTCCGGCAGGAGCAGTTACATGATTGCGGGCAGTATGGTGGCATTGGTCACACCCATGGATGCACAAGGGCGTGTTGACTGGGGCAGCCTCGACAAACTTGTAGACTTCCACCTGGAAAACGGCACCCATGCGATCGTCGCTGTCGGCACCACCGGTGAGTCGGCCACGCTGGATGTCGAAGAACACATCCTGGTCATCAAGCACGTGGTCGAGCGCGTCAAACGCAGCGCCAAACCGATTCCGGTCAT  >  NC_002947/1414669‑1414957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑