Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,416,868 T→A 8.6% L39Q (CTG→CAG)  PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,416,8680TA 8.6% 199.5 / 8.2 70L39Q (CTG→CAG) PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base T (31/33);  new base A (4/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

TGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAA  >  NC_002947/1416721‑1417015
                                                                                                                                                      |                                                                                                                                                   
tGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTg                                                                                                                                                    <  5:339970/149‑1 (MQ=255)
            ttCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCAGCG‑‑CCAGGCGCCGCTCg                                                                                                                                          <  5:12788/147‑1 (MQ=255)
                ggACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGttt                                                                                                                                    <  6:140843/149‑1 (MQ=255)
                 gACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTc                                                                                                                                   >  4:40050/1‑149 (MQ=255)
                 gACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTc                                                                                                                                   <  2:317173/149‑1 (MQ=255)
                  accaccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCt                                                                                                                                  >  3:105732/1‑149 (MQ=255)
                   ccaccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTg                                                                                                                                 <  5:106918/149‑1 (MQ=255)
                     accTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGca                                                                                                                               <  7:157685/149‑1 (MQ=255)
                        tGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGcagct                                                                                                                            >  7:165706/1‑149 (MQ=255)
                         gggCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGcagct                                                                                                                            >  6:14500/1‑148 (MQ=255)
                               aGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAgctcagctca                                                                                                                     <  8:165706/149‑1 (MQ=255)
                                      ttCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCTCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcagt                                                                                                              >  4:388570/1‑149 (MQ=255)
                                      ttCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcagt                                                                                                              >  7:101236/1‑149 (MQ=255)
                                          cgGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTg                                                                                                          <  4:68369/149‑1 (MQ=255)
                                            gTACTCGGAAGCGGCAGCCAAGGGATCGGCACTCTGATCGCCAGTGGTGACACGTTCATGCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGt                                                                                                        <  6:128010/149‑1 (MQ=255)
                                               cTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCa                                                                                                      <  6:158157/148‑1 (MQ=255)
                                               cTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCAc                                                                                                     <  1:340083/149‑1 (MQ=255)
                                                tCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTg                                                                                                          <  7:51686/143‑1 (MQ=255)
                                                tCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTg                                                                                                          >  8:51686/1‑143 (MQ=255)
                                                      gCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTgg                                                                                                         <  3:292076/138‑1 (MQ=255)
                                                      gCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTgg                                                                                                         >  4:292076/1‑138 (MQ=255)
                                                       cGGCAGCCAAGGGAACGGCACGCTGATCGCCAATGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGaa                                                                                              >  1:250776/1‑148 (MQ=255)
                                                               aaGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCTCTG‑CGTGTAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGAc                                                                                     <  3:388570/149‑1 (MQ=255)
                                                               aaGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGAc                                                                                     <  5:14500/149‑1 (MQ=255)
                                                                  ggAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGc                                                                                         >  7:57621/1‑142 (MQ=255)
                                                                  ggAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGc                                                                                         <  8:57621/142‑1 (MQ=255)
                                                                     aCGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTg                                                                               >  5:119459/1‑149 (MQ=255)
                                                                       ggCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCa                                                                             >  7:95033/1‑149 (MQ=255)
                                                                          aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGc                                                                                                                                <  5:53131/95‑1 (MQ=255)
                                                                          aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGc                                                                                                                                >  6:53131/1‑95 (MQ=255)
                                                                              tGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTc                                                                      >  3:172696/1‑149 (MQ=255)
                                                                                aTCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGCACGTGGTCg                                                                     <  8:101236/148‑1 (MQ=255)
                                                                                aTCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTc                                                                      <  6:118853/147‑1 (MQ=255)
                                                                                    ccAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCACTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTg                                                                >  6:216572/1‑149 (MQ=255)
                                                                                     cAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCGAAGCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCtt                                                                 >  1:322896/1‑149 (MQ=255)
                                                                                           tgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCAc                                                         <  6:324119/149‑1 (MQ=255)
                                                                                                 gTTCATCCTGGTCGTTTGCGGCTTTTCCCTG‑CGTTAAACCTAGCGGCG‑‑CCTGGCGCTGGTGGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCt                                                              <  2:198545/138‑1 (MQ=255)
                                                                                                 gTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCt                                                              >  1:198545/1‑138 (MQ=255)
                                                                                                 gTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgcgc                                                    >  4:57283/1‑148 (MQ=255)
                                                                                                 gTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgcgc                                                    >  3:385927/1‑148 (MQ=255)
                                                                                                 gTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCt                                                   <  8:95033/149‑1 (MQ=255)
                                                                                                     aTCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACaa                                               >  1:321423/1‑149 (MQ=255)
                                                                                                       ccTGGTCGATTGCGGCTTTTCCCTGCCGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTc                                                           >  1:212551/1‑136 (MQ=255)
                                                                                                       ccTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTc                                                           <  2:212551/135‑1 (MQ=255)
                                                                                                         tGGTCGATTGCGGCTTTTCCCTT‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGc                                                               <  8:127930/129‑1 (MQ=255)
                                                                                                         tGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGc                                                               >  7:127930/1‑129 (MQ=255)
                                                                                                         tGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTa                                           >  1:388743/1‑149 (MQ=255)
                                                                                                           gTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAcc                                         >  7:3516/1‑149 (MQ=255)
                                                                                                           gTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAc                                          <  3:57283/148‑1 (MQ=255)
                                                                                                            tCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                        <  5:216572/149‑1 (MQ=255)
                                                                                                              gATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCgg                                       >  4:93722/1‑148 (MQ=255)
                                                                                                                  gCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                  <  1:392112/133‑1 (MQ=255)
                                                                                                                  gCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                  >  2:392112/1‑133 (MQ=255)
                                                                                                                         ttCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGc                            >  5:7863/1‑148 (MQ=255)
                                                                                                                            ccTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAg                        >  7:235869/1‑149 (MQ=255)
                                                                                                                             cTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCTGTCTACCTCAGCCAAgg                       <  4:172696/149‑1 (MQ=255)
                                                                                                                                  gTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGCACGTGGTCGGc                                                                   <  6:319844/101‑1 (MQ=255)
                                                                                                                                  gTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGCACGTGGTCGGc                                                                   >  5:319844/1‑101 (MQ=255)
                                                                                                                                   tGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACtt                  <  8:3516/149‑1 (MQ=255)
                                                                                                                                     accccGAGCAGCG‑‑CCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATACCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                              >  6:129577/4‑135 (MQ=255)
                                                                                                                                     accccGAGCAGCG‑‑CCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATACCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                              <  5:129577/132‑1 (MQ=255)
                                                                                                                                       cccGAGCAGCG‑‑CCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcg              >  8:34529/2‑149 (MQ=255)
                                                                                                                                       cccGAGCAGCG‑‑CCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcg              >  6:190367/2‑149 (MQ=255)
                                                                                                                                        ccGAGCAGCG‑‑CCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcgc             >  1:43060/1‑149 (MQ=255)
                                                                                                                                         cGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCCGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGcgcg            <  3:93722/149‑1 (MQ=255)
                                                                                                                                            gcggcg‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACttt                 <  7:120237/141‑1 (MQ=255)
                                                                                                                                            gcggcg‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACttt                 >  8:120237/1‑141 (MQ=255)
                                                                                                                                              ggcg‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATg       <  6:119459/149‑1 (MQ=255)
                                                                                                                                                cg‑‑cCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATgcg     >  5:259984/1‑149 (MQ=255)
                                                                                                                                                     cTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCaa  <  2:250776/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTG‑CGTGAAACCGAGCGGCG‑‑CCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAA  >  NC_002947/1416721‑1417015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑