Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,484,997 A→G 5.2% I359V (ATC→GTC)  yhdX → amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,484,9970AG 5.2% 230.7 / 2.2 77I359V (ATC→GTC) yhdXamino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (38/35);  new base G (2/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CATCCCCCAGGCACTGCGGGTGATCATTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCAAACCCGATATGCCGC  >  NC_002947/1484849‑1485145
                                                                                                                                                    |                                                                                                                                                    
catcCCCCAGGCACTGCGGGTGATCATTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCggccaggcca                                                                                                                                                      >  4:289432/1‑149 (MQ=255)
     cccAGGCACTGCGGGTGATCATTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCACCCAGACCGGCCAGGCCATCGAg                                                                                                                                                 >  1:188729/1‑149 (MQ=255)
            aCTGCGGGTGATCATTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCg                                                                                                                                          >  3:296640/1‑149 (MQ=255)
                    tGATCATTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGTCCAGGCCATCGAGGTGATCGCCATCAc                                                                                                                                   >  1:197276/1‑148 (MQ=255)
                     gATCATTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCa                                                                                                                                 >  4:73976/1‑149 (MQ=255)
                         aTTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGa                                                                                                                              <  8:56079/148‑1 (MQ=255)
                         aTTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAg                                                                                                                             >  3:361223/1‑149 (MQ=255)
                         aTTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCGGGCGGCCTGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGa                                                                                                                              <  3:146624/148‑1 (MQ=255)
                         aTTCAGCCTCTGACCAGCCAGTACCTTAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGTCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGa                                                                                                                              <  2:281305/148‑1 (MQ=255)
                            ccgccgCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCTCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAgtg                                                                                                                           <  1:240373/148‑1 (MQ=255)
                              gccgcTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCt                                                                                                                        <  4:296640/149‑1 (MQ=255)
                                         gccagTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCAt                                                                                                                                >  4:75661/1‑130 (MQ=255)
                                         gccagTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCAt                                                                                                                                <  3:75661/130‑1 (MQ=255)
                                         gccagTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcat                                                                                                              >  2:231081/1‑148 (MQ=255)
                                          ccagTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTAt                                                                                                                      >  6:92105/1‑139 (MQ=255)
                                          ccagTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTAt                                                                                                                      <  5:92105/139‑1 (MQ=255)
                                          ccagTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcat                                                                                                              >  4:146892/1‑147 (MQ=255)
                                            agTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCGTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcatcag                                                                                                           >  7:206784/1‑148 (MQ=255)
                                            agTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCGTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcatcag                                                                                                           >  3:150335/1‑148 (MQ=255)
                                            agTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcatcagc                                                                                                          <  7:30691/149‑1 (MQ=255)
                                             gTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcatcagca                                                                                                         >  8:226295/1‑149 (MQ=255)
                                              tACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcatcagcat                                                                                                        <  1:231081/149‑1 (MQ=255)
                                                 cTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAg                                                                                                                                                 >  1:380427/1‑105 (MQ=255)
                                                 cTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAg                                                                                                                                                 <  2:380427/105‑1 (MQ=255)
                                                 cTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcatcagcatcag                                                                                                     >  7:318495/1‑149 (MQ=255)
                                                 cTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCcatcagcat                                                                                                        >  3:37383/1‑146 (MQ=255)
                                                   gAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCAtcagcatcagca                                                                                                   >  7:365576/1‑149 (MQ=255)
                                                   gAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCAtcagcatcagca                                                                                                   >  7:371291/1‑149 (MQ=255)
                                                       cTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTc                                                                                               >  2:158038/1‑149 (MQ=255)
                                                          gCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCgct                                                                                            <  4:338599/149‑1 (MQ=255)
                                                          gCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCg                                                                                              <  4:361223/147‑1 (MQ=255)
                                                           cGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCgctg                                                                                           <  3:146892/149‑1 (MQ=255)
                                                            gaagaaCTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATc                                                                                                                                           <  6:360708/100‑1 (MQ=255)
                                                            gaagaaCTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATc                                                                                                                                           >  5:360708/1‑100 (MQ=255)
                                                             aagaaCTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCgctgct                                                                                         >  5:94880/1‑149 (MQ=255)
                                                                aaCTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCtgat                                                                                      >  2:306848/1‑149 (MQ=255)
                                                                aaCTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCCCCATCAGCATCAGCATTTCGCTGCtgat                                                                                      >  3:160250/1‑149 (MQ=255)
                                                                 aCTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCtgat                                                                                      >  4:405372/1‑148 (MQ=255)
                                                                       cGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAAc                                                                                  <  8:365576/146‑1 (MQ=255)
                                                                       cGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAAc                                                                                  <  3:289432/146‑1 (MQ=255)
                                                                        gCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTgg                                                                               <  4:37383/148‑1 (MQ=255)
                                                                         cTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCGTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTa                                                                             <  4:150335/149‑1 (MQ=255)
                                                                         cTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCGTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTa                                                                             <  8:206784/149‑1 (MQ=255)
                                                                         cTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTa                                                                             <  8:318495/149‑1 (MQ=255)
                                                                                   gTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAgcgc                                                                   <  1:306848/149‑1 (MQ=255)
                                                                                      tCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCAtt                                                                >  5:145104/1‑149 (MQ=255)
                                                                                        ggCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACTGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCAt                                                                 <  1:136879/146‑1 (MQ=255)
                                                                                        ggCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCAt                                                                 >  2:136879/1‑146 (MQ=255)
                                                                                        ggCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTgc                                                              >  5:311915/1‑149 (MQ=255)
                                                                                         gCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTAtctc                                                                                                                   >  4:253163/1‑95 (MQ=255)
                                                                                         gCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTAtctc                                                                                                                   <  3:253163/95‑1 (MQ=255)
                                                                                         gCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTgcg                                                             <  8:371291/149‑1 (MQ=255)
                                                                                         gCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTgcg                                                             >  1:353757/1‑149 (MQ=255)
                                                                                          ttACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTgcgc                                                            <  2:187555/148‑1 (MQ=255)
                                                                                            aCCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCt                                                           >  5:139155/1‑148 (MQ=255)
                                                                                              ccGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTgcgc                                                            >  5:252243/1‑145 (MQ=255)
                                                                                              ccGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTgcgc                                                            <  6:252243/145‑1 (MQ=255)
                                                                                                   gaTGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGc                                                   <  3:405372/149‑1 (MQ=255)
                                                                                                    aTGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCg                                                  <  6:139155/149‑1 (MQ=255)
                                                                                                      ggTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGt                                                >  2:128568/1‑149 (MQ=255)
                                                                                                       gTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGt                                                >  5:249333/1‑148 (MQ=255)
                                                                                                          tcGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAg                                             >  1:320939/1‑148 (MQ=255)
                                                                                                          tcGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAg                                             >  6:128862/1‑148 (MQ=255)
                                                                                                                gtagCCGATACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGc                                      <  5:92562/146‑1 (MQ=255)
                                                                                                                    ccGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGt                                  <  6:311915/149‑1 (MQ=255)
                                                                                                                        tACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAAt                              <  6:94880/149‑1 (MQ=255)
                                                                                                                           cGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGcc                           >  4:101349/1‑149 (MQ=255)
                                                                                                                             tGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCTGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCa                         <  1:128568/149‑1 (MQ=255)
                                                                                                                                tCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCAt                        <  6:357827/147‑1 (MQ=255)
                                                                                                                                tCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCAt                        >  5:357827/1‑147 (MQ=255)
                                                                                                                                tCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGt                      >  6:68105/1‑149 (MQ=255)
                                                                                                                                tCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGt                      >  1:18430/1‑149 (MQ=255)
                                                                                                                                   tgcAGGCCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGttt                    >  6:118794/3‑148 (MQ=255)
                                                                                                                                   aCCAGACCGGCCAGGCCATCGAGGTGATCGCCATCAcc                                                                                                                                  <  1:408127/38‑1 (MQ=255)
                                                                                                                                   aCCAGACCGGCCAGGCCATCGAGGTGATCGCCATCAcc                                                                                                                                  >  2:408127/1‑38 (MQ=255)
                                                                                                                                    ccAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTc                  <  1:158038/149‑1 (MQ=255)
                                                                                                                                      aGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCaa                <  3:73976/149‑1 (MQ=255)
                                                                                                                                          cGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCAAAccc            <  6:249333/149‑1 (MQ=255)
                                                                                                                                            gccaggccaTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCaa                >  4:54480/1‑143 (MQ=255)
                                                                                                                                            gccaggccaTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCaa                <  3:54480/143‑1 (MQ=255)
                                                                                                                                            gccaggccaTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCAAACCCGa          >  6:92821/1‑149 (MQ=255)
                                                                                                                                               aggccaTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCAAACCCGatat       <  2:18430/149‑1 (MQ=255)
                                                                                                                                                    aTCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCAAACCCGATATgccgc  <  6:145104/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CATCCCCCAGGCACTGCGGGTGATCATTCCGCCGCTGACCAGCCAGTACCTGAACCTGGCGAAGAACTCTTCGCTGGCGGCCGGTATCGGCTACCCGGAGATGGTCTCGCTGTTCGCCGGTACCGTGCTCAACCAGACCGGCCAGGCCATCGAGGTGATCGCCATCACCATGAGTGTCTATCTCGCCATCAGCATCAGCATTTCGCTGCTGATGAACTGGTACAACAAGCGCATTGCGCTGATCGAGCGGTGAGGATACGCCCGTGAATGCCCATGTTTTCAAACCCGATATGCCGC  >  NC_002947/1484849‑1485145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑