Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,580,814 T→G 7.7% K195Q (AAG→CAG)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,580,8140TG 7.7% 157.3 / 4.7 52K195Q (AAG→CAG) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base G (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTG  >  NC_002947/1580669‑1580947
                                                                                                                                                 |                                                                                                                                     
gcggTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTAtt                                                                                                                                    <  1:364744/149‑1 (MQ=255)
gcggTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTAtt                                                                                                                                    <  4:186882/149‑1 (MQ=255)
       gCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCtt                                                                                                                              <  4:206711/148‑1 (MQ=255)
        cAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTgg                                                                                                                            <  7:53365/149‑1 (MQ=255)
             gggTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGa                                                                                                                       >  7:66407/1‑149 (MQ=255)
               gTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGAt                                                                                                                      >  4:86921/1‑148 (MQ=255)
               gTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGAt                                                                                                                      <  3:86921/148‑1 (MQ=255)
                               tggCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATg                                                                                                     <  2:335753/149‑1 (MQ=255)
                                 gCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGc                                                                                                   >  1:221723/1‑149 (MQ=255)
                                       aGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACt                                                                                              <  8:243185/148‑1 (MQ=255)
                                       aGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTg                                                                                             <  4:364047/149‑1 (MQ=255)
                                           gggTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCa                                                                                         >  7:32936/1‑149 (MQ=255)
                                           gggTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCa                                                                                         >  7:127188/1‑149 (MQ=255)
                                               gCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCg                                                                                      >  6:266997/1‑148 (MQ=255)
                                                cccGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGaa                                                                                    >  6:292163/1‑149 (MQ=255)
                                                         gCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGaa                                                                           <  2:221723/149‑1 (MQ=255)
                                                         gCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGaa                                                                           <  5:266997/149‑1 (MQ=255)
                                                          cTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTgg                                                                             >  6:55455/1‑146 (MQ=255)
                                                          cTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGaa                                                                           <  5:55455/148‑1 (MQ=255)
                                                            ggCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGa                                                                                                                   >  2:198278/1‑106 (MQ=255)
                                                            ggCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGa                                                                                                                   <  1:198278/106‑1 (MQ=255)
                                                            ggCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGt                                                                         >  5:182189/1‑148 (MQ=255)
                                                             gCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGc                                                                                                                 <  3:34773/107‑1 (MQ=255)
                                                             gCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGc                                                                                                                 >  4:34773/1‑107 (MQ=255)
                                                             gCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTc                                                                        <  8:32936/148‑1 (MQ=255)
                                                                    gccgAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTacac                                                                <  8:66407/149‑1 (MQ=255)
                                                                         gCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGc                                                                                                   <  1:178656/109‑1 (MQ=255)
                                                                         gCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGc                                                                                                   >  2:178656/1‑109 (MQ=255)
                                                                         gCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCcgcg                                                           <  5:292163/149‑1 (MQ=255)
                                                                                    gACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGAt                                                <  2:17646/149‑1 (MQ=255)
                                                                                     acacCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTAcgc                                                     >  8:49609/1‑143 (MQ=255)
                                                                                         cTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGcc                                                                                                                <  1:334375/80‑1 (MQ=255)
                                                                                         cTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGcc                                                                                                                >  2:334375/1‑80 (MQ=255)
                                                                                             aCGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCTGGTCCt                                        >  4:28874/1‑148 (MQ=255)
                                                                                                        ggCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCgaga                            <  8:127188/149‑1 (MQ=255)
                                                                                                         gCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGAc                           >  6:82310/1‑149 (MQ=255)
                                                                                                           cacaGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTGATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCTCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCggg                                            <  3:79468/130‑1 (MQ=255)
                                                                                                           cacaGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTGATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCggg                                            >  4:79468/1‑130 (MQ=255)
                                                                                                           cacaGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTGATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCggg                                            <  3:84977/130‑1 (MQ=255)
                                                                                                           cacaGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTGATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCCCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCggg                                            >  4:84977/1‑130 (MQ=255)
                                                                                                            acaGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCAt                                    <  2:263081/137‑1 (MQ=255)
                                                                                                            acaGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCAt                                    >  1:263081/1‑137 (MQ=255)
                                                                                                                  gTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGtt                  >  6:72178/1‑149 (MQ=255)
                                                                                                                  gTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGtt                  <  7:49609/149‑1 (MQ=255)
                                                                                                                          gTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGgt          >  8:214147/1‑149 (MQ=255)
                                                                                                                             gACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGata                       <  6:325549/133‑1 (MQ=255)
                                                                                                                             gACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGata                       >  5:325549/1‑133 (MQ=255)
                                                                                                                                  gggTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACTCCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTg  <  1:327130/149‑1 (MQ=255)
                                                                                                                                  gggTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTg  <  6:105947/149‑1 (MQ=255)
                                                                                                                                  gggTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTg  >  8:247400/1‑149 (MQ=255)
                                                                                                                                              aCTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGaa                                                                           >  2:136038/1‑64 (MQ=255)
                                                                                                                                              aCTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGaa                                                                           <  1:136038/64‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                     
GCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTG  >  NC_002947/1580669‑1580947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑