Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,580,997 T→C 85.2% K134E (AAG→GAG)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,580,9970TC85.2% 130.9 / 12.0 54K134E (AAG→GAG) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base C (26/20);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCA  >  NC_002947/1580855‑1581134
                                                                                                                                              |                                                                                                                                         
tGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGc                                                                                                                                     >  3:157525/1‑149 (MQ=255)
    cACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTg                                                                                                                                 <  2:203403/149‑1 (MQ=255)
      ccGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGt                                                                                                                               <  5:72178/149‑1 (MQ=255)
        gAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTg                                                                                                                                 >  8:230853/1‑145 (MQ=255)
        gAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTg                                                                                                                                 <  7:230853/145‑1 (MQ=255)
              cTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGa                                                                                                                       <  7:247400/149‑1 (MQ=255)
              cTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTCGGTCATGCTGCCGTCTTCGGAGACAAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACTCGGa                                                                                                                       >  6:334047/1‑149 (MQ=255)
               tGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTAGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATAACCAGCAGGAAGTACTCCACTGCTTTGGTGAAGCCGa                                                                                                                       >  4:105365/1‑148 (MQ=255)
                      cACCTACACCCGCGGTACGCGAGATAGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTtgc                                                                                                               >  5:309221/1‑149 (MQ=255)
                       aCCTACACACGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGACAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACAAGGCCGATCACCAGCAGGAAGTTCTCAACTGCTTTg                                                                                                                                 >  6:337293/1‑130 (MQ=255)
                         cTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTtgctgc                                                                                                            >  6:29532/1‑149 (MQ=255)
                           acacCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATAcc                                                                                                                   <  8:10347/140‑1 (MQ=255)
                           acacCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATAcc                                                                                                                   >  7:10347/1‑140 (MQ=255)
                             acCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCa                                                                                                        >  4:123259/1‑149 (MQ=255)
                               ccGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACt                                                                                                      >  3:230490/1‑149 (MQ=255)
                                      aCGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCg                                                                                               >  6:172185/1‑149 (MQ=255)
                                         cgAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCgg                                                                                              >  6:147365/1‑147 (MQ=255)
                                               cGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCg                                                                                      >  2:342586/1‑149 (MQ=255)
                                                gggTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCATGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCg                                                                                      <  6:309221/148‑1 (MQ=255)
                                                 ggTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCggg                                                                                    <  4:230490/149‑1 (MQ=255)
                                                       gCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTAGGAGACCAGGCCTATCACCAGCAGGAAGTTCTCCAATGATTTGGTGAAGAGGATACCTTGCTGCTGCACTTCCTGATTCAGAAGCGGGGTGGc                                                                               >  2:125591/1‑148 (MQ=255)
                                                          tGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAgg                                                                           >  8:20874/1‑149 (MQ=255)
                                                           gTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGc                                                                                                >  3:302352/1‑127 (MQ=255)
                                                           gTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGc                                                                                                <  4:302352/127‑1 (MQ=255)
                                                           gTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTAGGAGAAAAGGCCCATCACCAGCAGGAAGTTCTCCAGTCCGTTGGTGACGCGGAACCATTGCTGCTGCACTTCCTGCGGCAGCAGCGGGTTGGCGAGGt                                                                          >  8:323690/1‑149 (MQ=255)
                                                               gagaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCa                                                                       <  7:20874/148‑1 (MQ=255)
                                                                agaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCTTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGc                                                                     <  1:342586/149‑1 (MQ=255)
                                                                agaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGc                                                                     <  3:105365/149‑1 (MQ=255)
                                                                     atatAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGtt                                                                <  3:123259/149‑1 (MQ=255)
                                                                     atatAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGtt                                                                <  2:385628/149‑1 (MQ=255)
                                                                           ttGGCCAGGTCGTACTTTGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTTGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTctgtac                                                          <  2:144993/149‑1 (MQ=255)
                                                                                      gtcCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTgc                                                <  5:29532/148‑1 (MQ=255)
                                                                                         cTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCGACTGCTTTGGTGACGCGGATACATTGCTGCTGCACTTCCTGCGGCAGAAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACATGTAACTGCGCgg                                            >  5:283433/1‑149 (MQ=255)
                                                                                         cTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCgg                                            >  3:71623/1‑149 (MQ=255)
                                                                                          ttGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGgt                                           >  1:314592/1‑149 (MQ=255)
                                                                                              tCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCg                                       >  6:337184/1‑149 (MQ=255)
                                                                                               cATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGtt                                                                <  6:67774/123‑1 (MQ=255)
                                                                                                    tgcCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTg                                 >  5:326234/1‑149 (MQ=255)
                                                                                                     gcCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCggg                                                                                    <  5:407393/97‑1 (MQ=255)
                                                                                                     gcCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCggg                                                                                    >  6:407393/1‑97 (MQ=255)
                                                                                                      ccGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTg                                 <  2:314592/147‑1 (MQ=255)
                                                                                                        gTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGc                             >  2:295855/1‑149 (MQ=255)
                                                                                                         tCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGcc                            <  5:172185/149‑1 (MQ=255)
                                                                                                             cctagaCCAGGCCGATCACCCGCAGGAAGTTCTCCACTGCGTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTg                         <  6:326234/145‑1 (MQ=255)
                                                                                                              ggAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGtt                       >  8:62971/1‑149 (MQ=255)
                                                                                                              ggAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGtt                       >  2:130399/1‑149 (MQ=255)
                                                                                                                   ccAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAg                  <  8:165901/149‑1 (MQ=255)
                                                                                                                     aGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGt                >  2:171914/1‑149 (MQ=255)
                                                                                                                       gCCGATCACCAGCAGGAAGTTCTCCACTGCTATGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAATGTgg              <  4:71623/149‑1 (MQ=255)
                                                                                                                                cagcagGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTg                                 <  2:403285/121‑1 (MQ=255)
                                                                                                                                cagcagGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTg                                 >  1:403285/1‑121 (MQ=255)
                                                                                                                                cagcagGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATgg     >  1:373143/1‑149 (MQ=255)
                                                                                                                                   cagGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCa  >  7:296000/1‑149 (MQ=255)
                                                                                                                                   cagGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCa  <  5:337184/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                         
TGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCA  >  NC_002947/1580855‑1581134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑