Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,591,711 G→C 8.1% I61M (ATC→ATG) ‡ PP_1395 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,591,7110GC 8.1% 196.1 / 5.8 61I61M (ATC→ATG) ‡PP_1395AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (34/22);  new base C (3/2);  total (37/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCGCAGGCCCAGGCTTGGGTTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGT  >  NC_002947/1591564‑1591858
                                                                                                                                                   |                                                                                                                                                   
caTGCGCAGGCCCAGGCTTGGGTTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAgg                                                                                                                                                     <  8:34762/148‑1 (MQ=255)
caTGCGCAGGCCCAGGCTTGGGTTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAgg                                                                                                                                                     <  5:6266/148‑1 (MQ=255)
caTGCGCAGGCCCAGGCTTGGGTTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGa                                                                                                                                                    <  7:236339/149‑1 (MQ=255)
    cgcAGGCCCAGGCTTGGGGTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGca                                                                                                                                                <  8:169864/149‑1 (MQ=255)
           ccAGGCTTGGGTTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAggg                                                                                                                                           >  6:203034/1‑147 (MQ=255)
           ccAGGCTTGGGTTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAggg                                                                                                                                           >  2:26723/1‑147 (MQ=255)
                     gTTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTgcgc                                                                                                                                 >  3:189990/1‑147 (MQ=255)
                         tcGGGGCTGGCATCTAGCCTCAAGGCGGTGAGTGGCAATGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAgggt                                                                                                                           <  8:340289/149‑1 (MQ=255)
                         tcGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAgggt                                                                                                                           >  6:281251/1‑149 (MQ=255)
                           ggggCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAgggt                                                                                                                           <  3:232783/147‑1 (MQ=255)
                            gggCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAgggtggg                                                                                                                        <  1:26723/149‑1 (MQ=255)
                               cTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTa                                                                                                                      >  8:63667/1‑148 (MQ=255)
                                  gCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACa                                                                                                                    <  5:203034/147‑1 (MQ=255)
                                               cGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGAt                                                                                                                                                   <  1:82256/103‑1 (MQ=255)
                                               cGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGAt                                                                                                                                                   >  2:82256/1‑103 (MQ=255)
                                               cGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCg                                                                                                     <  6:402526/149‑1 (MQ=255)
                                                 gcgcTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGgt                                                                                                   <  7:63667/149‑1 (MQ=255)
                                                 gcgcTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGgt                                                                                                   <  7:145248/149‑1 (MQ=255)
                                                     tGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTaa                                                                                               >  7:356218/1‑149 (MQ=255)
                                                           gCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCAc                                                                                          >  1:402237/1‑148 (MQ=255)
                                                            cAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCa                                                                                        <  4:24119/149‑1 (MQ=255)
                                                                   aCCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAg                                                                                 >  7:308672/1‑149 (MQ=255)
                                                                     cGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAgg                                                                                      <  4:224822/142‑1 (MQ=255)
                                                                     cGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAgg                                                                                      >  3:224822/1‑142 (MQ=255)
                                                                     cGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAgg                                                                                >  5:404030/1‑148 (MQ=255)
                                                                     cGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGt                                                                               >  4:198308/1‑149 (MQ=255)
                                                                             ccATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATg                                                                       <  5:281251/149‑1 (MQ=255)
                                                                             ccATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATg                                                                       >  1:168515/1‑149 (MQ=255)
                                                                                          tGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGAc                                                          <  8:356218/149‑1 (MQ=255)
                                                                                          tGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTG‑CCTTGGCAAAGAAGCACAAGGCAGGCAGCGCCAGGGTTGGTACAGAAAGTACATTTTAGGTGTTACGCACCAGGGACAAGTCATAGAATGCCCAAGCTTGAc                                                          >  2:136490/1‑148 (MQ=255)
                                                                                              tCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCa                                                        >  6:277304/1‑147 (MQ=255)
                                                                                              tCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCa                                                        >  2:93873/1‑147 (MQ=255)
                                                                                                  aGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGc                                                  >  8:327011/1‑149 (MQ=255)
                                                                                                    gCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTc                                                >  2:229806/1‑149 (MQ=255)
                                                                                                         cTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAgg                                                                                                                             >  4:265967/1‑67 (MQ=255)
                                                                                                         cTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAgg                                                                                                                             <  3:265967/67‑1 (MQ=255)
                                                                                                             tCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATg                                                                       >  1:278900/1‑117 (MQ=255)
                                                                                                             tCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATg                                                                       <  2:278900/117‑1 (MQ=255)
                                                                                                             tCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATg                                       >  2:32615/1‑149 (MQ=255)
                                                                                                             tCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATg                                       >  3:95082/1‑149 (MQ=255)
                                                                                                              cGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATgg                                      >  1:358339/1‑149 (MQ=255)
                                                                                                               gTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTAc                                                                                                                     >  2:328632/1‑69 (MQ=255)
                                                                                                               gTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTAc                                                                                                                     <  1:328632/69‑1 (MQ=255)
                                                                                                               gTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGCCCTAAGGGCTCGCCCGCAAGGc                                     >  8:384333/1‑149 (MQ=255)
                                                                                                               gTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGc                                     <  5:213334/149‑1 (MQ=255)
                                                                                                                  aCCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCgg                                   <  4:189990/148‑1 (MQ=255)
                                                                                                                    ccAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCACCCGCATGGCGGtt                                 >  1:236927/1‑148 (MQ=255)
                                                                                                                        gAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCa                            <  2:168515/149‑1 (MQ=255)
                                                                                                                            aGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTc                        >  6:204831/1‑149 (MQ=255)
                                                                                                                              gcgcagcctgcCCTGTGCATCCTTGCCCAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCgg                      >  6:335432/8‑149 (MQ=38)
                                                                                                                              gCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCgg                      >  4:1846/1‑149 (MQ=255)
                                                                                                                               cgcagcctgcCCTGTGCATCCTTGCCCAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGc                     <  5:335432/143‑1 (MQ=255)
                                                                                                                                ttttGCTGCCCTGGGCAAGGATGCACAGGGCAGGCAGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGc                     >  1:268745/1‑148 (MQ=255)
                                                                                                                                  agcctgcCCTGTGCATCCTTGCCCAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAg                  >  6:172323/4‑149 (MQ=255)
                                                                                                                                    gctgcCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCt                >  7:29436/1‑149 (MQ=255)
                                                                                                                                    cctgcCCTGTGCATCCTTGCCCAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTg                                                             >  7:200816/2‑104 (MQ=38)
                                                                                                                                    cctgcCCTGTGCATCCTTGCCCAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTg                                                             <  8:200816/103‑1 (MQ=38)
                                                                                                                                      tgcCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTc               >  6:75856/1‑148 (MQ=255)
                                                                                                                                               cAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGCTCAGCTCGGCCAGCTCCAGGCGCTg      >  8:92260/1‑148 (MQ=255)
                                                                                                                                               cAAGGATGCACAGGGCAGGCTGCACCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCaaa                                                      >  5:357521/1‑100 (MQ=255)
                                                                                                                                               cAAGGATGCACAGGGCAGGCTGCACCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCaaa                                                      <  6:357521/100‑1 (MQ=255)
                                                                                                                                                aaGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTg      >  3:196091/1‑147 (MQ=255)
                                                                                                                                                aaGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTg      <  4:196091/147‑1 (MQ=255)
                                                                                                                                                  ggATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTCATCAGCTCGGCCAGCTCCAGGCGCTGCAGt  <  1:32615/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATGCGCAGGCCCAGGCTTGGGTTCTCGGGGCTGGCATCCAGCCTCACGGCGCTGAGTGGCAAGGTCACCGAAACGACCATGTAATGCAGTGGGTCGTAGGCGTACTGCTCGTCACCCAGGAACAGGCTTTTGCTGCCCTGGGCAAGGATGCACAGGGCAGGCTGCGCCAGGGTGGGTACAGAACGTACATTTTCGGTGTAACGCACCAGGTACAGGTCATCGATGGCCGATGCTGGACCAAAGGGCTCGCCCGCATGGCGGTTGATCAGCTCGGCCAGCTCCAGGCGCTGCAGT  >  NC_002947/1591564‑1591858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑