Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,598,492 G→A 5.5% A90A (GCC→GCT) ‡ dctB ← C4‑dicarboxylate transport sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,598,4920GA 5.5% 280.6 / 3.9 91A90A (GCC→GCT) ‡dctBC4‑dicarboxylate transport sensor protein
Reads supporting (aligned to +/- strand):  ref base G (41/45);  new base A (3/2);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

AGTAGGGGCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTGCTGGCTGGCACGCTT  >  NC_002947/1598347‑1598638
                                                                                                                                                 |                                                                                                                                                  
aGTAGGGGCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCg                                                                                                                                                 <  8:198409/149‑1 (MQ=255)
 gTAGGGGCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGt                                                                                                                                                >  4:55531/1‑149 (MQ=255)
  tAGGGGCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGtt                                                                                                                                               >  4:316182/1‑149 (MQ=255)
    ggggCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTc                                                                                                                                              <  7:351032/148‑1 (MQ=255)
    ggggCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTc                                                                                                                                              <  1:68831/148‑1 (MQ=255)
    ggggCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCt                                                                                                                                             <  4:381749/149‑1 (MQ=255)
     gggCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTg                                                                                                                                            <  7:347221/149‑1 (MQ=255)
      ggCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGa                                                                                                                                           <  1:51873/149‑1 (MQ=255)
        cGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAAc                                                                                                                                         <  4:344306/149‑1 (MQ=255)
         ggAAACCGTAGTTGGCGCCGACGTAGCTGCTGCGCTGCAGCCAGTTGCTGTCGGCTATGGACAGCCCGGTGCGGTGGAGCAGCTCGAGGGTGGATGAATTGGCGTCGCCATTGATGCGTTCGAGCTTGAGGTTCAGAACGCCCTGAAcc                                                                                                                                        <  5:404510/149‑1 (MQ=255)
            aaCCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGAACGCCCTGAACCtca                                                                                                                                     <  4:196509/149‑3 (MQ=255)
             aCCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCtttt                                                                                                                                    >  8:332687/1‑149 (MQ=255)
               cGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTtcg                                                                                                                                  >  8:84940/1‑149 (MQ=255)
                    ttGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGAc                                                                                                                             >  2:165693/1‑149 (MQ=255)
                    ttGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGAc                                                                                                                             >  4:147618/1‑149 (MQ=255)
                      ggAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCCTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCg                                                                                                                           >  7:71508/1‑149 (MQ=255)
                            gACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCcgc                                                                                                                     <  1:200367/149‑1 (MQ=255)
                              cGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCgcgc                                                                                                                   <  1:263882/149‑1 (MQ=255)
                                 aGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAg                                                                                                                >  7:144377/1‑149 (MQ=255)
                                    tgctgGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAggg                                                                                                                                                   <  4:33697/111‑1 (MQ=255)
                                    tgctgGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAggg                                                                                                                                                   >  3:33697/1‑111 (MQ=255)
                                      ctgGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTg                                                                                                            <  8:144377/148‑1 (MQ=255)
                                      ctgGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGc                                                                                                           >  2:209578/1‑149 (MQ=255)
                                        gggCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGa                                                                                                         >  2:264509/1‑149 (MQ=255)
                                        gggCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGAGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTtc                                                                                                                                   >  4:333336/1‑123 (MQ=255)
                                        gggCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGAGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTtc                                                                                                                                   <  3:333336/123‑1 (MQ=255)
                                         ggCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGTTGCGAt                                                                                                        <  3:147618/149‑1 (MQ=255)
                                          gCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCTCAAGGCTGCGATc                                                                                                       <  1:165693/149‑1 (MQ=255)
                                          gCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATc                                                                                                       <  3:23106/149‑1 (MQ=255)
                                          gCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATc                                                                                                       <  7:332687/149‑1 (MQ=255)
                                          gCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATc                                                                                                       <  5:99180/149‑1 (MQ=255)
                                            aGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAg                                                                                                     <  2:291347/149‑1 (MQ=255)
                                              ccgccAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCt                                                                                                   >  1:64519/1‑149 (MQ=255)
                                               cgccAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTc                                                                                                  >  2:2415/1‑149 (MQ=255)
                                                gccAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTcc                                                                                                 >  6:212557/1‑149 (MQ=255)
                                                gccAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCCTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTcc                                                                                                 <  8:71508/149‑1 (MQ=255)
                                                  cAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCgg                                                                                               >  1:21472/1‑149 (MQ=255)
                                                       gCTGGCGGCAATGGCCAGCCCGGTGTGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTc                                                                                            >  4:384209/1‑147 (MQ=255)
                                                         tGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAgc                                                                                        >  1:264673/1‑149 (MQ=255)
                                                           gcggcAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTg                                                                                                            >  8:71925/1‑127 (MQ=255)
                                                           gcggcAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTg                                                                                                            <  7:71925/127‑1 (MQ=255)
                                                           gcggcAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTc                                                                                            <  6:341937/143‑1 (MQ=255)
                                                           gcggcAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTc                                                                                            >  5:341937/1‑143 (MQ=255)
                                                           gcggcAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAgc                                                                                        <  2:51719/147‑1 (MQ=255)
                                                           gcggcAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAgc                                                                                        <  8:17/147‑1 (MQ=255)
                                                                aaTGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGtt                                                                                                                                               >  3:67846/1‑87 (MQ=255)
                                                                aaTGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGtt                                                                                                                                               <  4:67846/87‑1 (MQ=255)
                                                                aaTGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCggg                                                                                              >  7:146204/1‑136 (MQ=255)
                                                                aaTGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCggg                                                                                              <  8:146204/136‑1 (MQ=255)
                                                                aaTGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCAATGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCa                                                                                 >  1:111507/1‑149 (MQ=255)
                                                                    gCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCAcggc                                                                             >  6:82655/1‑149 (MQ=255)
                                                                      cAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCAcggcgg                                                                           <  1:2415/149‑1 (MQ=255)
                                                                      cAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCAcggcgg                                                                           <  5:212557/149‑1 (MQ=255)
                                                                      cAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCAcggcgg                                                                           <  2:264673/149‑1 (MQ=255)
                                                                      cAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCAcggcgg                                                                           >  1:287862/1‑149 (MQ=255)
                                                                      cAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAACTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCAGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTc                                                                                            >  4:157546/1‑132 (MQ=255)
                                                                      cAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAACTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCAGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTc                                                                                            <  3:157546/132‑1 (MQ=255)
                                                                          ccGGTGGGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAg                                                                       <  5:207259/149‑1 (MQ=255)
                                                                           cGGTGCGGTCGAGCAGCTCGGGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAgg                                                                      <  1:17005/149‑1 (MQ=255)
                                                                                 ggTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACgg                                                                <  2:64519/149‑1 (MQ=255)
                                                                                    cGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAg                                                             <  3:316182/149‑1 (MQ=255)
                                                                                     gAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGc                                                            >  8:83217/1‑149 (MQ=255)
                                                                                     gAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGc                                                            >  4:344535/1‑149 (MQ=255)
                                                                                      agcagcTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGa                                                                        <  3:78053/136‑1 (MQ=255)
                                                                                      agcagcTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGa                                                                        >  4:78053/1‑136 (MQ=255)
                                                                                       gcagcTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGcga                                                          <  8:53930/149‑1 (MQ=255)
                                                                                            tCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGcgatcgat                                                     >  1:235060/1‑149 (MQ=255)
                                                                                                    ggaggaATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGgtgt                                             >  8:178357/1‑149 (MQ=255)
                                                                                                     gaggaATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGtgtg                                            <  3:384209/149‑1 (MQ=255)
                                                                                                       ggaATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGtgtg                                            >  5:247165/1‑147 (MQ=255)
                                                                                                        gaATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACAGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAg                                         >  5:221319/1‑149 (MQ=255)
                                                                                                          aTTGGCGGAGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCCCGGCGGGGAGGGCACGGTCGCGATCGATCAGTGTGTGCCGTg                                       >  8:159721/1‑149 (MQ=255)
                                                                                                              gcggcgCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGtt                                   >  8:190328/1‑149 (MQ=255)
                                                                                                                  cgcCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCg                               <  7:190328/149‑1 (MQ=255)
                                                                                                                  cgcCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCg                               >  6:421693/1‑149 (MQ=255)
                                                                                                                          aTGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGTTCAGGGTGTGCAGTGCGTTGGCGTACAGGcc                       >  7:156554/1‑149 (MQ=255)
                                                                                                                           tGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGcc                                                                                     <  6:118780/86‑1 (MQ=255)
                                                                                                                              gTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGt                   >  5:387718/1‑149 (MQ=255)
                                                                                                                               ttCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGcgatcgat                                                     <  2:38174/114‑1 (MQ=255)
                                                                                                                               ttCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGtt                  <  5:82655/149‑1 (MQ=255)
                                                                                                                                 cGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTg                 <  2:21472/148‑1 (MQ=255)
                                                                                                                                  gAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTgc                <  7:83217/148‑1 (MQ=255)
                                                                                                                                   acgaaaaGGTTCAGAACGCCCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTgctg              >  7:338285/7‑149 (MQ=255)
                                                                                                                                     cTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTgctggc            <  8:156554/149‑1 (MQ=255)
                                                                                                                                       tGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTgctggctg          >  5:155280/1‑149 (MQ=255)
                                                                                                                                       aaaGGTTCAGAACGCCCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTgctggctg          >  6:31165/3‑149 (MQ=255)
                                                                                                                                       aaaGGTTCAGAACGCCCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTgctggctg          >  5:390975/3‑149 (MQ=255)
                                                                                                                                        gAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTgctggct           <  1:264509/147‑1 (MQ=255)
                                                                                                                                        gAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTgctggct           <  1:209578/147‑1 (MQ=255)
                                                                                                                                        gAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTGctggctgg         >  4:339924/1‑149 (MQ=255)
                                                                                                                                           gTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCATTGGCGTACAGGCCCAGTTGCTGGCTGGCAc      >  3:45801/1‑149 (MQ=255)
                                                                                                                                             tCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTGCTGGCTGGCACGc    >  6:341655/1‑149 (MQ=255)
                                                                                                                                               aGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTGCTGGCTGGCACGCtt  <  3:344535/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGTAGGGGCGGAAACCGTAGTTGGAGCCGACGTAGCTGCTGGGCAGCCGCCAGTTGCTGGCGGCAATGGCCAGCCCGGTGCGGTCGAGCAGCTCGAGGGTGGAGGAATTGGCGGCGCCATTGATGCGTTCGAGCTTGAGGTTCAGGGCGTTCTGAACCTTTTCGTCGACCGGGCCGCGCAAGGCTGCGATCAGCTCCGGGTCCAGCGCCAGCACGGCGGGGAGGGCACGGTAGCGATCGATCAGGGTGTGCAGTGCGTTGGCGTACAGGCCCAGTTGCTGGCTGGCACGCTT  >  NC_002947/1598347‑1598638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑