Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,746,273 A→T 5.3% S65T (TCA→ACA)  PP_1544 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,746,2730AT 5.3% 231.5 / 2.5 75S65T (TCA→ACA) PP_1544hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/35);  new base T (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

CACTTCGCACCCTGGGTCTCGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGCGGTCTCGG  >  NC_002947/1746130‑1746421
                                                                                                                                               |                                                                                                                                                     
cACTTCGCACCCTGGGTCTCGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAgc                                                                                                                                                   <  4:171931/148‑1 (MQ=255)
  cTTCGCACCCTGGGTCTCGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGcc                                                                                                                                                <  4:25197/149‑1 (MQ=255)
   ttCGCACCCTGGGTCTCGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAgcgc                                                                                                                                                 >  2:387743/1‑147 (MQ=255)
   ttCGCACCCTGGGTCTCGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAgcgc                                                                                                                                                 >  6:65180/1‑147 (MQ=255)
           cTGGGTCTCGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGc                                                                                                                                       <  3:7731/149‑1 (MQ=255)
            tGGGTCTCGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGc                                                                                                                                       >  7:215515/1‑148 (MQ=255)
                  tcGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGTCAGCGCCAGGTCCTGCCAGAGt                                                                                                                                 >  8:132728/1‑148 (MQ=255)
                    gCTTAAGCTCTGCCATGCTTGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTcc                                                                                                                              <  5:263453/149‑1 (MQ=255)
                    gCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTc                                                                                                                               <  8:144854/148‑1 (MQ=255)
                    gCTTAAGCTCCGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTcc                                                                                                                              >  4:336629/1‑149 (MQ=255)
                     cTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCt                                                                                                                             <  3:257162/149‑1 (MQ=255)
                              tGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGt                                                                                                                   >  4:113700/1‑149 (MQ=255)
                               gCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTgg                                                                                                                    <  3:202781/147‑1 (MQ=255)
                               gCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTgg                                                                                                                    >  4:202781/1‑147 (MQ=255)
                                ccATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCg                                                                                                                 >  2:332463/1‑149 (MQ=255)
                                ccATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCg                                                                                                                 <  3:113700/149‑1 (MQ=255)
                                 cATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGt                                                                                                                <  1:404022/149‑1 (MQ=255)
                                   tGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAg                                                                                                              >  2:209594/1‑149 (MQ=255)
                                        gCCGCTTCGAACTCACGCGCTAGTTTTGGCTATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTg                                                                                                         <  7:388651/149‑1 (MQ=255)
                                         ccGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCa                                                                                                                                     >  8:134051/1‑121 (MQ=255)
                                         ccGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCa                                                                                                                                     <  7:134051/121‑1 (MQ=255)
                                                  aCTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGAtc                                                                                               >  1:277809/1‑149 (MQ=255)
                                                    tCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGAtctc                                                                                             >  2:16841/1‑149 (MQ=255)
                                                      aCGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGAtc                                                                                               <  2:21679/145‑1 (MQ=255)
                                                      aCGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGAtc                                                                                               >  1:21679/1‑145 (MQ=255)
                                                      aCGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCg                                                                                           >  2:167325/1‑149 (MQ=255)
                                                      aCGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCg                                                                                           >  8:28381/1‑149 (MQ=255)
                                                          gcTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGTCAgc                                                                                                                                                   >  1:165408/1‑90 (MQ=255)
                                                          gcTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGTCAgc                                                                                                                                                   <  2:165408/90‑1 (MQ=255)
                                                              gTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCa                                                                                     <  8:215515/147‑1 (MQ=255)
                                                              gTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATg                                                                                   <  5:65180/149‑1 (MQ=255)
                                                               ttttGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATgg                                                                                  >  2:148805/1‑149 (MQ=255)
                                                               ttttGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATgg                                                                                  >  3:138629/1‑149 (MQ=255)
                                                                          tGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGTCAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGAt                                                                        <  7:132728/148‑1 (MQ=255)
                                                                           gTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATcc                                                                      >  3:228373/1‑149 (MQ=255)
                                                                            tAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATccc                                                                     >  8:264767/1‑149 (MQ=255)
                                                                             aaaaaGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCt                                                                                                    <  5:285298/117‑1 (MQ=255)
                                                                             aaaaaGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCt                                                                                                    >  6:285298/1‑117 (MQ=255)
                                                                             aaaaaGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCt                                                                    >  4:326819/1‑149 (MQ=255)
                                                                             aaaaaGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCt                                                                    >  4:188806/1‑149 (MQ=255)
                                                                                aaGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTcc                                                                 <  1:289809/149‑1 (MQ=255)
                                                                                       cgcgTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGt                                                          <  4:138629/149‑1 (MQ=255)
                                                                                           tCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTgaa                                                      <  1:167325/149‑1 (MQ=255)
                                                                                           tCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTg                                                        >  4:187591/1‑147 (MQ=255)
                                                                                            cAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTgaa                                                      <  3:326819/148‑1 (MQ=255)
                                                                                             aGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTgaag                                                     <  7:28381/148‑1 (MQ=255)
                                                                                               gTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTgaagaa                                                   >  1:242384/1‑148 (MQ=255)
                                                                                                tCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGaaaa                                                 <  1:16841/149‑1 (MQ=255)
                                                                                                 cGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAAc                                                >  1:315798/1‑149 (MQ=255)
                                                                                                  gTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAAc                                                <  2:277809/148‑1 (MQ=255)
                                                                                                   ttCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCt                                              >  2:23750/1‑149 (MQ=255)
                                                                                                     cAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCATGTACTGCCAGAGTTCCTTCACCCTTGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCt                                              <  1:332463/148‑1 (MQ=18)
                                                                                                         gCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGa                                        >  3:187941/1‑149 (MQ=255)
                                                                                                           gACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATc                                      <  2:71712/149‑1 (MQ=255)
                                                                                                             cgcgCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATccc                                    <  2:242384/149‑1 (MQ=255)
                                                                                                                  ccGCTCTTCAGGATCCTGCGCGATCCATGAGAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGtt                               >  1:243733/1‑149 (MQ=255)
                                                                                                                   cGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGTATGTTGAAGAAAACCTTCTCGATCCCCAGttt                              >  5:69542/1‑149 (MQ=255)
                                                                                                                   cGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGttt                              <  8:348669/149‑1 (MQ=255)
                                                                                                                   cGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGttt                              >  2:87148/1‑149 (MQ=255)
                                                                                                                                   gcgcGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCttcttc              <  1:96333/149‑1 (MQ=255)
                                                                                                                                    cgcgATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTtcttct             >  2:297248/1‑149 (MQ=255)
                                                                                                                                     gcgATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTACTg            >  7:64635/1‑149 (MQ=255)
                                                                                                                                       gATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGTATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGc          <  6:69542/149‑1 (MQ=255)
                                                                                                                                       gATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGc          <  7:80360/149‑1 (MQ=255)
                                                                                                                                       gATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGc          <  1:148805/149‑1 (MQ=255)
                                                                                                                                        aTCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGCg         <  2:243733/149‑1 (MQ=255)
                                                                                                                                        aTCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGCg         <  1:23750/149‑1 (MQ=255)
                                                                                                                                            aTGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTg                                                        >  4:363059/1‑98 (MQ=255)
                                                                                                                                            aTGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTg                                                        <  3:363059/98‑1 (MQ=255)
                                                                                                                                            aTGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAAcc                                               <  4:262040/107‑1 (MQ=255)
                                                                                                                                            aTGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAAcc                                               >  3:262040/1‑107 (MQ=255)
                                                                                                                                            aTGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTtct                                           >  3:223015/1‑111 (MQ=255)
                                                                                                                                            aTGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTtct                                           <  4:223015/111‑1 (MQ=255)
                                                                                                                                            aTGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGCGGtct     >  4:129653/1‑149 (MQ=255)
                                                                                                                                              gACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGCGGTCTCg   >  7:317065/1‑149 (MQ=255)
                                                                                                                                               aCAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGCGGTCTCgg  <  2:315798/149‑1 (MQ=255)
                                                                                                                                               aCAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGCGGTCTCgg  <  4:187941/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
CACTTCGCACCCTGGGTCTCGCTTAAGCTCTGCCATGCTGGCCGCTTCGAACTCACGCGCTAGTTTTGGCGATATGTAAAAAGCTGGCGCGTCAGGTCGTTCAAGGCGACGCGCCCGCTCTTCAGGATCCTGCGCGATCCATGACAGCGCCAGGTCCTGCCAGAGTTCCTGCA‑CCTGGTCGTAGCCGTGCCGCTCGATCTCCGCAGCCATGGCAGCCTTGATCCCTTCCGGCATGTTGAAGAAAACCTTCTCGATCCCCAGTTTCGCGGCTGCCTTCTTCTGGCGGTCTCGG  >  NC_002947/1746130‑1746421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑