Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,748,903	A→G	6.1%	intergenic (‑236/‑96)	PP_1548 ← / → PP_5744	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,748,903	0	A	G	6.1%	243.6 / 4.5	82	intergenic (‑236/‑96)	PP_1548/PP_5744	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/41);  new base G (3/2);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

GACATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCT  >  NC_002947/1748758‑1749045                                                                                                                                                  |                                                                                                                                                  gACATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACtt                                                                                                                                                <  3:395886/149‑1 (MQ=255)   cATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGc                                                                                                                                              <  7:241935/149‑1 (MQ=255)   cATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGc                                                                                                                                              <  6:334958/149‑1 (MQ=255)      gTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCAtt                                                                                                                                           >  3:93812/1‑149 (MQ=255)          acctactaaGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTta                                                                                                                                         <  1:255703/140‑1 (MQ=255)                  aGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGaa                                                                                                                                                   >  8:321732/1‑129 (MQ=255)                  aGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGaa                                                                                                                                                   <  7:321732/129‑1 (MQ=255)                  aGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATg                                                                                                                               <  6:368907/149‑1 (MQ=255)                    cTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCt                                                                                                                             >  2:400347/1‑149 (MQ=255)                       gCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTACTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCtt                                                                                                                            <  8:136882/147‑1 (MQ=255)                        cTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑t                                                                                                                         <  1:83813/148‑1 (MQ=255)                         tACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATATGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTAGATGAAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTAG‑ttt                                                                                                                       >  8:130208/1‑149 (MQ=255)                                cGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTaaa                                                                                                                 <  5:186704/148‑1 (MQ=255)                                cGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAAt                                                                                                                <  3:373154/149‑1 (MQ=255)                                 gTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTAGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAGGCTTG‑GTTTTTAAATg                                                                                                               >  5:415694/1‑149 (MQ=255)                                  tAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTTGCTTCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGc                                                                                                              <  3:194628/149‑1 (MQ=255)                                          accaGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAAGCATGCTTTTATAAATGCAAgttcgc                                                                                                      >  3:337879/1‑144 (MQ=25)                                          accaGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGc                                                                                                    <  6:175712/149‑1 (MQ=255)                                            caGAACTCAATCGGCGGATTAAGTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGGTGCCAGCTCGATGCAAATGCAAGCGAACTTGGATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCtt                                                                                                  <  7:130208/149‑1 (MQ=255)                                               aaCTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTtgtg                                                                                               >  3:139043/1‑149 (MQ=255)                                                 cTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGc                                                                                                              <  3:26231/134‑1 (MQ=255)                                                 cTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGc                                                                                                              >  4:26231/1‑134 (MQ=255)                                                    aaTCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAAGCATGCTTTTATAAATGCAAGTT‑‑CGCTtgcattt                                                                                            <  4:337879/147‑6 (MQ=21)                                                    aaTCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAAtt                                                                                          <  4:93812/149‑1 (MQ=255)                                                    aaTCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAAtt                                                                                          >  7:116358/1‑149 (MQ=255)                                                            gATTAACACGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTa                                                                                  <  8:363100/149‑1 (MQ=255)                                                                   ttgagttcTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATg                                                                           <  5:25789/142‑1 (MQ=255)                                                                   tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGTTCGCTTGCAttt                                                                                                                                          >  1:281767/1‑89 (MQ=25)                                                                   tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGTTCGCTTGCAttt                                                                                                                                          <  2:281767/89‑1 (MQ=25)                                                                   tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑tttttt                                                                                                                    <  8:24335/110‑1 (MQ=255)                                                                   tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑tttttt                                                                                                                    >  7:24335/1‑110 (MQ=255)                                                                     ggCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATg                                                                           <  4:15946/147‑1 (MQ=255)                                                                        gcCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGt                                                                      >  3:396263/1‑149 (MQ=255)                                                                           tggtggCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCa                                                                   >  1:372183/1‑149 (MQ=255)                                                                             gtggCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAgt                                                                 <  4:396263/149‑1 (MQ=255)                                                                             gtggCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAg                                                                  <  1:400347/148‑1 (MQ=255)                                                                                gCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAgtgt                                                               <  8:116358/148‑1 (MQ=255)                                                                                  tCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCAGTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAgtgtg                                                              >  6:274682/1‑147 (MQ=255)                                                                                    aTCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAg                                                           <  2:372183/148‑1 (MQ=255)                                                                                     tCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAg                                                           >  8:167797/1‑147 (MQ=255)                                                                                       tgctgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATgg                                                                          <  3:403803/130‑1 (MQ=255)                                                                                       tgctgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATgg                                                                          >  4:403803/1‑130 (MQ=255)                                                                                           ggTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGATTGAGTAATTTTCTCTTACTGTATGGATGTCCAGAGTGTAGGAGCTTTc                                                   >  2:198494/1‑149 (MQ=255)                                                                                            gTTTTTAAAGAGCGGTGGCTGCCGGAGCTGGCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCg                                                  >  1:32343/1‑149 (MQ=255)                                                                                            gTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCg                                                  >  1:361076/1‑149 (MQ=255)                                                                                            gTTTTTAAAGAGCGGTGGCTGCCGGAGATGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCg                                                  >  1:16954/1‑149 (MQ=255)                                                                                             tttttAAAGCGCGGTCGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCgg                                                 <  2:351201/149‑1 (MQ=255)                                                                                             tttttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGGATGGATGTCCAGTGTGTAGGAGCTTTCg                                                  >  5:369737/1‑148 (MQ=255)                                                                                              ttttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCATTTAGTTGTTTAATTTTCTATTTCTGTATGTTTGTCCAGTGTTTTGGAGGtttgg                                                  >  1:337098/1‑147 (MQ=255)                                                                                               tttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGc                                                                                                              <  4:247315/88‑1 (MQ=255)                                                                                               tttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGc                                                                                                              >  3:247315/1‑88 (MQ=255)                                                                                               tttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGAt                                               >  4:137234/1‑149 (MQ=255)                                                                                                    agagCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGAt                                                                        >  8:189785/1‑119 (MQ=255)                                                                                                    agagCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGAt                                                                        <  7:189785/119‑1 (MQ=255)                                                                                                    agagCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCgg                                          >  5:417373/1‑149 (MQ=255)                                                                                                     gagCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAATTGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGgc                                         >  7:25481/1‑149 (MQ=255)                                                                                                     gagCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAgtgt                                                               >  4:13803/1‑127 (MQ=255)                                                                                                     gagCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAgtgt                                                               <  3:13803/127‑1 (MQ=255)                                                                                                     gagCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGgc                                         <  2:361076/149‑1 (MQ=255)                                                                                                       gCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGgcgc                                       <  3:137234/149‑1 (MQ=255)                                                                                                        cGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCa                                      <  6:369737/149‑1 (MQ=255)                                                                                                         ggTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAATTGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAg                                     <  8:25481/149‑1 (MQ=255)                                                                                                           tGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTc                                                   <  1:14843/133‑1 (MQ=255)                                                                                                           tGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTc                                                   >  2:14843/1‑133 (MQ=255)                                                                                                                    gAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGt                          >  3:291889/1‑149 (MQ=255)                                                                                                                          gcAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                    <  2:32343/148‑1 (MQ=255)                                                                                                                          ccAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTa                     <  4:291889/148‑1 (MQ=255)                                                                                                                          ccAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                    >  3:176438/1‑149 (MQ=255)                                                                                                                              cTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGaa                <  2:16954/149‑1 (MQ=255)                                                                                                                               tCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATgg                  >  5:35920/1‑146 (MQ=255)                                                                                                                                ttttataaatgcaaGTTCGCTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAaga              >  2:355762/7‑149 (MQ=255)                                                                                                                                 gATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAagag             >  2:122606/1‑149 (MQ=255)                                                                                                                                   tgcaaatgcaaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGaa                <  7:155373/144‑1 (MQ=255)                                                                                                                                   tgcaaatgcaaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGaa                >  8:155373/1‑144 (MQ=255)                                                                                                                                   tgcaaatgcaaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGc            <  1:119047/148‑1 (MQ=255)                                                                                                                                        atgcaaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGa      <  4:139043/149‑1 (MQ=255)                                                                                                                                           caaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGcc   >  6:49948/1‑149 (MQ=255)                                                                                                                                           caaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAAATTTCTCTTACTGTATGGATGTCCAGTGTGAAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGcc   <  6:377773/149‑1 (MQ=255)                                                                                                                                            aaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTc                                                   >  6:230134/1‑100 (MQ=255)                                                                                                                                            aaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTc                                                   <  5:230134/100‑1 (MQ=255)                                                                                                                                            aaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCg                                                  <  2:380379/101‑1 (MQ=255)                                                                                                                                            aaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCg                                                  >  1:380379/1‑101 (MQ=255)                                                                                                                                            aaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATTGAAGAGCTAACGAgagt  >  8:181213/1‑146 (MQ=255)                                                                                                                                            aaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCt  >  8:19420/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GACATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCA‑‑CGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCT  >  NC_002947/1748758‑1749045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑