Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,786,961	C→T	7.5%	G264G (GGC→GGT)	tsf →	elongation factor Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,786,961	0	C	T	7.5%	272.4 / 7.5	92	G264G (GGC→GGT)	tsf	elongation factor Ts
Reads supporting (aligned to +/- strand):  ref base C (44/41);  new base T (3/4);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTA  >  NC_002947/1786814‑1787109                                                                                                                                                    |                                                                                                                                                     gaACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGc                                                                                                                                                      >  1:2174/1‑148 (MQ=255)    cATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAg                                                                                                                                                  <  8:389852/149‑1 (MQ=255)    cATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAg                                                                                                                                                  >  7:56347/1‑149 (MQ=255)     aTGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAgg                                                                                                                                                 >  3:239390/1‑149 (MQ=255)         tCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                                             <  5:41170/149‑1 (MQ=255)          cAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                                             >  6:130570/1‑148 (MQ=255)           aaCGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCga                                                                                                                                           <  4:136837/149‑1 (MQ=255)               gTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAAGCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCgagcat                                                                                                                                       >  7:143478/1‑146 (MQ=255)                     tCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGt                                                                                                                                  <  3:52612/148‑1 (MQ=255)                     tCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTc                                                                                                                                 <  8:56347/149‑1 (MQ=255)                        ccAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTcgac                                                                                                                              >  5:108669/1‑149 (MQ=255)                           aGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCgacgac                                                                                                                           >  4:91405/1‑149 (MQ=255)                              tCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACtt                                                                                                                         <  3:118914/148‑1 (MQ=255)                                aaGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCgc                                                                                                                      >  3:149594/1‑149 (MQ=255)                                   gCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCgctgc                                                                                                                   >  8:346305/1‑149 (MQ=255)                                     cGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTcgac                                                                                                                              <  4:364896/136‑1 (MQ=255)                                     cGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTcgac                                                                                                                              >  3:364896/1‑136 (MQ=255)                                       aaGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGaa                                                                                                               <  5:130570/149‑1 (MQ=255)                                           cTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCAt                                                                                                                                              >  6:370245/1‑114 (MQ=255)                                           cTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCAt                                                                                                                                              <  5:370245/114‑1 (MQ=255)                                           cTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAg                                                                                                                                   >  4:265073/1‑125 (MQ=255)                                           cTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAg                                                                                                                                   <  3:265073/125‑1 (MQ=255)                                            tCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTg                                                                                                           >  4:264692/1‑148 (MQ=255)                                            tCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGc                                                                                                          >  8:155477/1‑149 (MQ=255)                                              gCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTg                                                                                                           <  3:264692/146‑1 (MQ=255)                                              gCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGc                                                                                                          <  6:270018/147‑1 (MQ=255)                                                  aaaGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCt                                                                                                      >  7:65099/1‑147 (MQ=255)                                                  aaaGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCAGCTGAAGTTGCCGc                                                                                                       >  7:373642/1‑146 (MQ=255)                                                  aaaGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCAGCTGAAGTTGCCGc                                                                                                       <  8:373642/146‑1 (MQ=255)                                                       gCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAg                                                                                                   <  2:37992/145‑1 (MQ=255)                                                       gCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAg                                                                                                   >  1:37992/1‑145 (MQ=255)                                                        cAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCg                                                                                                        <  5:334826/139‑1 (MQ=255)                                                        cAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCg                                                                                                        >  6:334826/1‑139 (MQ=255)                                                          aGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCgctg                                                                                            >  5:396613/1‑149 (MQ=255)                                                             cTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGctgctg                                                                                         <  2:2174/149‑1 (MQ=255)                                                                cGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCtgctgc                                                                                        <  5:177667/147‑1 (MQ=255)                                                                      gAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAg                                                                                    <  4:22699/145‑1 (MQ=255)                                                                      gAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAg                                                                                    >  3:22699/1‑145 (MQ=255)                                                                       aaCCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                                                <  2:126895/148‑1 (MQ=255)                                                                          ccGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGa                                                                            >  6:416522/1‑149 (MQ=255)                                                                          ccGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGa                                                                            >  1:299633/1‑149 (MQ=255)                                                                            ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATGGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACa                                                                          >  1:291081/1‑149 (MQ=255)                                                                            ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGa                                                                            >  7:291225/1‑147 (MQ=255)                                                                                     ggTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCt                                                                 <  6:29719/149‑1 (MQ=255)                                                                                        tGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTc                                                              >  7:289467/1‑149 (MQ=255)                                                                                          gCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGc                                                            >  7:80865/1‑149 (MQ=255)                                                                                            gAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                                                >  8:203452/1‑127 (MQ=255)                                                                                            gAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                                                <  7:203452/127‑1 (MQ=255)                                                                                              gCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGcccc                                                         >  7:231677/1‑148 (MQ=255)                                                                                                  gCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCaaa                                                      >  1:13258/1‑147 (MQ=255)                                                                                                   ccAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTTGCTGCTGAAGTTGCCACTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAagag                                                   <  1:332138/149‑1 (MQ=255)                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAg                                                                             >  1:371275/1‑122 (MQ=255)                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAg                                                                             <  2:371275/122‑1 (MQ=255)                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAgg                                                  <  8:231677/149‑1 (MQ=255)                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAgg                                                  <  7:155477/149‑1 (MQ=255)                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCACTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAgg                                                  >  6:321438/1‑149 (MQ=255)                                                                                                     aaGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGc                                                 >  2:51834/1‑149 (MQ=255)                                                                                                     aaGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGc                                                 >  3:259452/1‑149 (MQ=255)                                                                                                      aGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCt                                                <  4:259452/149‑1 (MQ=255)                                                                                                              ggTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTc                                        <  8:65099/149‑1 (MQ=255)                                                                                                                   aGAAATCGTTTCCATCACCTACTTAAAAGTAGGCGAAGGCAACGAGAAGCCAGTCGACGACATCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCAGTCTGTAGCCCCAAAGAGGCTGCACGCTCAcgcg                                   >  2:176623/1‑149 (MQ=255)                                                                                                                      aaTCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCCGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACgcgcgc                                <  7:346305/149‑1 (MQ=255)                                                                                                                           tttCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAaga                                                    >  3:303662/1‑124 (MQ=255)                                                                                                                           tttCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAaga                                                    <  4:303662/124‑1 (MQ=255)                                                                                                                           tttCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCct                           <  5:416522/149‑1 (MQ=255)                                                                                                                             tCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCctc                          <  8:289467/148‑1 (MQ=255)                                                                                                                               cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCttt                       <  2:291081/149‑1 (MQ=255)                                                                                                                                ttCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTg                      >  8:33803/1‑149 (MQ=255)                                                                                                                                ttCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTg                      >  3:152736/1‑149 (MQ=255)                                                                                                                                ttCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTg                      >  7:346930/1‑149 (MQ=255)                                                                                                                                 tccccTACTTTCAAGAAGTTGAAGTCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGt                     <  6:48426/146‑1 (MQ=255)                                                                                                                                  cgccTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGt                     <  6:54503/146‑1 (MQ=255)                                                                                                                                    ccTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGc                                              >  6:68529/1‑121 (MQ=255)                                                                                                                                    ccTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGc                                              <  5:68529/121‑1 (MQ=255)                                                                                                                                     cTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCaa                  >  6:191877/1‑148 (MQ=255)                                                                                                                                      tACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAcgc                                    >  5:104432/1‑129 (MQ=255)                                                                                                                                      tACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAcgc                                    <  6:104432/129‑1 (MQ=255)                                                                                                                                        cgtCAAAGTCGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAAc               <  8:143478/146‑1 (MQ=255)                                                                                                                                        cTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCACTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAAc               <  5:321438/148‑1 (MQ=255)                                                                                                                                         ttCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCACAAAGAgg                                                  >  1:156353/1‑112 (MQ=255)                                                                                                                                         ttCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCACAAAGAgg                                                  <  2:156353/112‑1 (MQ=255)                                                                                                                                          tCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCttt                       <  7:373738/138‑1 (MQ=255)                                                                                                                                          tCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCttt                       >  8:373738/1‑138 (MQ=255)                                                                                                                                           cAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACggg            >  7:151663/1‑148 (MQ=255)                                                                                                                                           cAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACggg            <  8:80865/148‑1 (MQ=255)                                                                                                                                           cAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACgg             <  8:346930/147‑1 (MQ=255)                                                                                                                                            aaaGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACggg            >  2:206250/1‑147 (MQ=255)                                                                                                                                             aaGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGgga          <  4:152736/148‑1 (MQ=255)                                                                                                                                             aaGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGggag         >  1:253594/1‑149 (MQ=255)                                                                                                                                              aGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGggag         >  5:417609/1‑148 (MQ=255)                                                                                                                                                  ggCGAATGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGtt    <  4:239390/149‑1 (MQ=255)                                                                                                                                                   gCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGtt    <  5:191877/148‑1 (MQ=255)                                                                                                                                                   gCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGtt    >  2:381962/1‑148 (MQ=255)                                                                                                                                                    cGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCACTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCGGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTta  <  6:108669/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTA  >  NC_002947/1786814‑1787109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑