Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,806,106	C→G	7.1%	A4G (GCG→GGG) ‡	PP_1609 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,806,106	0	C	G	7.1%	252.3 / 7.8	83	A4G (GCG→GGG) ‡	PP_1609	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (36/40);  new base G (2/4);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CTGCTGGCGGTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGG  >  NC_002947/1805961‑1806251                                                                                                                                                  |                                                                                                                                                   ctgctgGCGGTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGa                                                                                                                                                  >  6:175229/1‑148 (MQ=255)         ggTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAg                                                                                                                                          >  6:241279/1‑148 (MQ=255)         ggTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGCAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAg                                                                                                                                          >  2:236738/1‑148 (MQ=255)          gTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACACCTTCTGTGGGTTCCGCGAAATCACAg                                                                                                                                          >  6:270413/1‑147 (MQ=255)               cAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑Gc                                                                                                                                  <  3:404929/149‑1 (MQ=255)                 aCCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGTTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtat                                                                                                                                <  5:180198/149‑4 (MQ=37)                 aCCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtat                                                                                                                                <  8:68246/149‑4 (MQ=38)                   cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatca                                                                                                                              <  1:110171/149‑6 (MQ=37)                   cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatc                                                                                                                               >  2:110171/1‑144 (MQ=37)                   cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGCAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGAt                                                                                                                              <  1:236738/149‑1 (MQ=255)                   cTGCCCGGGCTGGTGCAGGCGGATTACCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatca                                                                                                                              <  5:206314/149‑6 (MQ=37)                   cTGCCCGGGCTGGTGCAGGCGGATTACCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatc                                                                                                                               >  6:206314/1‑144 (MQ=37)                      cccGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTAc                                                                                                                           <  6:349699/149‑1 (MQ=255)                       ccGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTAcc                                                                                                                          <  1:215947/149‑1 (MQ=255)                         gggCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCaa                                                                                                                        <  2:226095/149‑1 (MQ=255)                           gCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAg                                                                                                                       >  2:4728/1‑148 (MQ=255)                                tGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGa                                                                                                                                        <  4:53877/127‑1 (MQ=255)                                tGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGa                                                                                                                                        >  3:53877/1‑127 (MQ=255)                                   aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                              >  7:331203/1‑149 (MQ=255)                                   aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                              <  5:241279/149‑1 (MQ=255)                                   aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                              <  4:167257/149‑1 (MQ=255)                                   aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                              <  3:152917/149‑1 (MQ=255)                                   aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                              <  1:81083/149‑1 (MQ=255)                                       ggATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGcc                                                                                                           >  5:143020/1‑148 (MQ=255)                                       ggATTGCCAACTGCACTGGCAGATACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGAATACCATCCATAATTGATACAAATTGTGAGGGTACCGAAAAAACACAAGAGTG‑GCCGATAACCAAGAAGttttttcac                                                                                                           >  7:260821/1‑145 (MQ=255)                                        gATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGcc                                                                                                           >  2:131037/1‑147 (MQ=255)                                               aaCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAAcaca                                                                                                                                           >  3:286782/1‑109 (MQ=255)                                               aaCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAAcaca                                                                                                                                           <  4:286782/109‑1 (MQ=255)                                                aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTc                                                                                                 <  2:354559/149‑1 (MQ=255)                                                aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTc                                                                                                 <  1:46801/149‑1 (MQ=255)                                                aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTATGAGCTGAAGGGTACATTCCGGTAGACTCCCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTCCCAAGCAGCTTTTTGCCGAGCTGATTc                                                                                                 <  2:280610/149‑1 (MQ=255)                                                      cTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGc                                                                                                                      <  3:319439/122‑1 (MQ=255)                                                      cTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGc                                                                                                                      >  4:319439/1‑122 (MQ=255)                                                       tGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAAt                                                                                          >  5:60607/1‑149 (MQ=255)                                                         gCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGa                                                                                        <  5:175229/149‑1 (MQ=255)                                                                 cgacgaACGCGCAAGGTTTGAGCTCAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTACGCGAAAACACAGGAGTT‑GCCGATTAACAAGCAGTTTTTTGCCGAGATGATTCTGGAAATTACGatcta                                                                                 >  7:257863/1‑144 (MQ=255)                                                                     gaACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGc                                                                             >  7:202364/1‑148 (MQ=255)                                                                           gcAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCg                                                                      <  6:29251/149‑1 (MQ=255)                                                                            cAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCgg                                                                     >  3:317617/1‑149 (MQ=255)                                                                             aaGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCggg                                                                    <  8:260821/149‑1 (MQ=255)                                                                              aGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGt                                                                   <  5:346942/149‑1 (MQ=255)                                                                                gTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGta                                                                  >  6:265853/1‑148 (MQ=255)                                                                                   tGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAc                                                               <  6:60607/148‑1 (MQ=255)                                                                                        tGAAGGGTACATTCCGGTAGACTACCGTGCCTTCGTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGc                                                         >  1:11936/1‑149 (MQ=255)                                                                                        tGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGAATACCCTGGc                                                         >  1:141457/1‑149 (MQ=255)                                                                                         gAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCt                                                        >  3:205451/1‑149 (MQ=255)                                                                                          aaGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑TCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCt                                                        <  6:143020/148‑1 (MQ=255)                                                                                               tACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATg                                                                                         <  1:44337/110‑1 (MQ=255)                                                                                               tACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATg                                                                                         >  2:44337/1‑110 (MQ=255)                                                                                                 cATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGTTAACACAGGAGTT‑GCCGAATACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGa                                                 <  1:131037/148‑1 (MQ=255)                                                                                                  aTTCCGGTAGACTACCCTCCCTTCTTGATACAACATCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGATTTATGCCCAGCTGATTCTGGAAATGACGAGCGAACGCCATTCCGGGTATACCCTGCCAGTACAGAcc                                               >  5:146847/1‑148 (MQ=255)                                                                                                            aCTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGGCGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGtttt                                     >  6:351096/1‑149 (MQ=255)                                                                                                            aCTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGttt                                      <  8:331203/148‑1 (MQ=255)                                                                                                              tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAccc                                                             <  5:307530/123‑1 (MQ=255)                                                                                                              tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAccc                                                             >  6:307530/1‑123 (MQ=255)                                                                                                              tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                   >  6:58723/1‑149 (MQ=255)                                                                                                                 cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGCCGCGGCAGTTTTTc                                   >  2:328322/1‑146 (MQ=255)                                                                                                                 cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                   >  8:232708/1‑146 (MQ=255)                                                                                                                 cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                   <  6:192848/146‑1 (MQ=255)                                                                                                                 cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                   >  5:192848/1‑146 (MQ=255)                                                                                                                  ctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCgaga                               <  1:4728/149‑1 (MQ=255)                                                                                                                      cttcttGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTg                                                                                               >  5:124745/1‑81 (MQ=255)                                                                                                                      cttcttGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTg                                                                                               <  6:124745/81‑1 (MQ=255)                                                                                                                            gATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACtg                        <  5:58723/146‑1 (MQ=255)                                                                                                                             aTACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAt                    >  1:277740/1‑149 (MQ=255)                                                                                                                             aTACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCAGGGTATACCATGGCTGTACAGACGAGGCAGTTTTTCGCTCTGCACTGTGAt                    >  4:297527/1‑149 (MQ=255)                                                                                                                              tACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAtt                   >  4:47232/1‑149 (MQ=255)                                                                                                                              tACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAt                    <  5:265853/148‑1 (MQ=255)                                                                                                                               aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAg                                                                                    <  1:18526/83‑1 (MQ=255)                                                                                                                               aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAg                                                                                    >  2:18526/1‑83 (MQ=255)                                                                                                                               aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGAc                                                <  4:92868/119‑1 (MQ=255)                                                                                                                               aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGAc                                                >  3:92868/1‑119 (MQ=255)                                                                                                                                cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTTGGCCGATAACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGACCGAACCCCATCCCGGGTATACCATGGATTTACAGACCCGGCAGTTTTTCGAGATGCACTTTGAtt                   >  1:268217/1‑148 (MQ=255)                                                                                                                                cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTg                  <  5:31872/148‑1 (MQ=255)                                                                                                                                cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGa                 >  1:6984/1‑149 (MQ=255)                                                                                                                                  aCTTCTGTGGGTTCCGCGTAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACg               <  2:268217/149‑1 (MQ=255)                                                                                                                                   cTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGc              <  2:1639/149‑1 (MQ=255)                                                                                                                                    ttCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCa             <  2:11936/149‑1 (MQ=255)                                                                                                                                         tgGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAgg           <  7:232708/146‑1 (MQ=255)                                                                                                                                         tgGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAgg           <  8:202364/146‑1 (MQ=255)                                                                                                                                          gggTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGa                                                                                     <  1:24164/71‑1 (MQ=255)                                                                                                                                          gggTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGa                                                                                     >  2:24164/1‑71 (MQ=255)                                                                                                                                               ccGCGAAAACACAGGAGTT‑GCCGATTACCAAGAAGTTTTTTGCCGAGCTGATTCTGGAAATGACTAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGTCGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCggg   <  8:257863/148‑1 (MQ=255)                                                                                                                                                cgcgAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGGCGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCgggg  <  5:351096/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CTGCTGGCGGTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGG  >  NC_002947/1805961‑1806251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑