Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,806,111	A→C	7.2%	T6P (ACA→CCA)	PP_1609 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,806,111	0	A	C	7.2%	253.5 / 6.7	83	T6P (ACA→CCA)	PP_1609	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/41);  new base C (2/4);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

GGTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCGG  >  NC_002947/1805969‑1806258                                                                                                                                               |                                                                                                                                                     ggTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAg                                                                                                                                                 >  6:241279/1‑148 (MQ=255) ggTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGCAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAg                                                                                                                                                 >  2:236738/1‑148 (MQ=255)  gTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACACCTTCTGTGGGTTCCGCGAAATCACAg                                                                                                                                                 >  6:270413/1‑147 (MQ=255)       cAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑Gc                                                                                                                                         <  3:404929/149‑1 (MQ=255)         aCCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGTTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtat                                                                                                                                       <  5:180198/149‑4 (MQ=37)         aCCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtat                                                                                                                                       <  8:68246/149‑4 (MQ=38)           cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatca                                                                                                                                     <  1:110171/149‑6 (MQ=37)           cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatc                                                                                                                                      >  2:110171/1‑144 (MQ=37)           cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGCAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGAt                                                                                                                                     <  1:236738/149‑1 (MQ=255)           cTGCCCGGGCTGGTGCAGGCGGATTACCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatca                                                                                                                                     <  5:206314/149‑6 (MQ=37)           cTGCCCGGGCTGGTGCAGGCGGATTACCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatc                                                                                                                                      >  6:206314/1‑144 (MQ=37)              cccGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTAc                                                                                                                                  <  6:349699/149‑1 (MQ=255)               ccGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTAcc                                                                                                                                 <  1:215947/149‑1 (MQ=255)                 gggCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCaa                                                                                                                               <  2:226095/149‑1 (MQ=255)                   gCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAg                                                                                                                              >  2:4728/1‑148 (MQ=255)                        tGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGa                                                                                                                                               >  3:53877/1‑127 (MQ=255)                        tGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGa                                                                                                                                               <  4:53877/127‑1 (MQ=255)                           aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                     <  1:81083/149‑1 (MQ=255)                           aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                     <  5:241279/149‑1 (MQ=255)                           aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                     <  3:152917/149‑1 (MQ=255)                           aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                     >  7:331203/1‑149 (MQ=255)                           aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                     <  4:167257/149‑1 (MQ=255)                               ggATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGcc                                                                                                                  >  5:143020/1‑148 (MQ=255)                               ggATTGCCAACTGCACTGGCAGATACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGAATACCATCCATAATTGATACAAATTGTGAGGGTACCGAAAAAACACAAGAGTG‑GCCGATAACCAAGAAGttttttcac                                                                                                                  >  7:260821/1‑145 (MQ=255)                                gATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGcc                                                                                                                  >  2:131037/1‑147 (MQ=255)                                       aaCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAAcaca                                                                                                                                                  <  4:286782/109‑1 (MQ=255)                                       aaCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAAcaca                                                                                                                                                  >  3:286782/1‑109 (MQ=255)                                        aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTc                                                                                                        <  2:354559/149‑1 (MQ=255)                                        aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTc                                                                                                        <  1:46801/149‑1 (MQ=255)                                        aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTATGAGCTGAAGGGTACATTCCGGTAGACTCCCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTCCCAAGCAGCTTTTTGCCGAGCTGATTc                                                                                                        <  2:280610/149‑1 (MQ=255)                                              cTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGc                                                                                                                             >  4:319439/1‑122 (MQ=255)                                              cTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGc                                                                                                                             <  3:319439/122‑1 (MQ=255)                                               tGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAAt                                                                                                 >  5:60607/1‑149 (MQ=255)                                                 gCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGa                                                                                               <  5:175229/149‑1 (MQ=255)                                                         cgacgaACGCGCAAGGTTTGAGCTCAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTACGCGAAAACACAGGAGTT‑GCCGATTAACAAGCAGTTTTTTGCCGAGATGATTCTGGAAATTACGatcta                                                                                        >  7:257863/1‑144 (MQ=255)                                                             gaACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGc                                                                                    >  7:202364/1‑148 (MQ=255)                                                                   gcAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCg                                                                             <  6:29251/149‑1 (MQ=255)                                                                    cAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCgg                                                                            >  3:317617/1‑149 (MQ=255)                                                                     aaGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCggg                                                                           <  8:260821/149‑1 (MQ=255)                                                                      aGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGt                                                                          <  5:346942/149‑1 (MQ=255)                                                                        gTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGta                                                                         >  6:265853/1‑148 (MQ=255)                                                                           tGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAc                                                                      <  6:60607/148‑1 (MQ=255)                                                                                tGAAGGGTACATTCCGGTAGACTACCGTGCCTTCGTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGc                                                                >  1:11936/1‑149 (MQ=255)                                                                                tGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGAATACCCTGGc                                                                >  1:141457/1‑149 (MQ=255)                                                                                 gAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCt                                                               >  3:205451/1‑149 (MQ=255)                                                                                  aaGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑TCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCt                                                               <  6:143020/148‑1 (MQ=255)                                                                                       tACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATg                                                                                                >  2:44337/1‑110 (MQ=255)                                                                                       tACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATg                                                                                                <  1:44337/110‑1 (MQ=255)                                                                                         cATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGTTAACACAGGAGTT‑GCCGAATACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGa                                                        <  1:131037/148‑1 (MQ=255)                                                                                          aTTCCGGTAGACTACCCTCCCTTCTTGATACAACATCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGATTTATGCCCAGCTGATTCTGGAAATGACGAGCGAACGCCATTCCGGGTATACCCTGCCAGTACAGAcc                                                      >  5:146847/1‑148 (MQ=255)                                                                                                    aCTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGGCGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGtttt                                            >  6:351096/1‑149 (MQ=255)                                                                                                    aCTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGttt                                             <  8:331203/148‑1 (MQ=255)                                                                                                      tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAccc                                                                    <  5:307530/123‑1 (MQ=255)                                                                                                      tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAccc                                                                    >  6:307530/1‑123 (MQ=255)                                                                                                      tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                          >  6:58723/1‑149 (MQ=255)                                                                                                         cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGCCGCGGCAGTTTTTc                                          >  2:328322/1‑146 (MQ=255)                                                                                                         cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                          >  5:192848/1‑146 (MQ=255)                                                                                                         cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                          >  8:232708/1‑146 (MQ=255)                                                                                                         cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                          <  6:192848/146‑1 (MQ=255)                                                                                                          ctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCgaga                                      <  1:4728/149‑1 (MQ=255)                                                                                                              cttcttGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTg                                                                                                      <  6:124745/81‑1 (MQ=255)                                                                                                              cttcttGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTg                                                                                                      >  5:124745/1‑81 (MQ=255)                                                                                                                    gATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACtg                               <  5:58723/146‑1 (MQ=255)                                                                                                                     aTACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAt                           >  1:277740/1‑149 (MQ=255)                                                                                                                     aTACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCAGGGTATACCATGGCTGTACAGACGAGGCAGTTTTTCGCTCTGCACTGTGAt                           >  4:297527/1‑149 (MQ=255)                                                                                                                      tACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAtt                          >  4:47232/1‑149 (MQ=255)                                                                                                                      tACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAt                           <  5:265853/148‑1 (MQ=255)                                                                                                                       aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAg                                                                                           <  1:18526/83‑1 (MQ=255)                                                                                                                       aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAg                                                                                           >  2:18526/1‑83 (MQ=255)                                                                                                                       aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGAc                                                       <  4:92868/119‑1 (MQ=255)                                                                                                                       aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGAc                                                       >  3:92868/1‑119 (MQ=255)                                                                                                                        cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTTGGCCGATAACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGACCGAACCCCATCCCGGGTATACCATGGATTTACAGACCCGGCAGTTTTTCGAGATGCACTTTGAtt                          >  1:268217/1‑148 (MQ=255)                                                                                                                        cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTg                         <  5:31872/148‑1 (MQ=255)                                                                                                                        cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGa                        >  1:6984/1‑149 (MQ=255)                                                                                                                          aCTTCTGTGGGTTCCGCGTAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACg                      <  2:268217/149‑1 (MQ=255)                                                                                                                           cTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGc                     <  2:1639/149‑1 (MQ=255)                                                                                                                            ttCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCa                    <  2:11936/149‑1 (MQ=255)                                                                                                                                 tgGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAgg                  <  7:232708/146‑1 (MQ=255)                                                                                                                                 tgGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAgg                  <  8:202364/146‑1 (MQ=255)                                                                                                                                  gggTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGa                                                                                            >  2:24164/1‑71 (MQ=255)                                                                                                                                  gggTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGa                                                                                            <  1:24164/71‑1 (MQ=255)                                                                                                                                       ccGCGAAAACACAGGAGTT‑GCCGATTACCAAGAAGTTTTTTGCCGAGCTGATTCTGGAAATGACTAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGTCGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCggg          <  8:257863/148‑1 (MQ=255)                                                                                                                                        cgcgAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGGCGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCgggg         <  5:351096/148‑1 (MQ=255)                                                                                                                                              aaCAAATGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACCGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCgg  <  3:297527/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     GGTGGCCAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCGG  >  NC_002947/1805969‑1806258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑