Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,806,117	G→A	7.4%	E8K (GAG→AAG)	PP_1609 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,806,117	0	G	A	7.4%	234.9 / 6.4	81	E8K (GAG→AAG)	PP_1609	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/43);  new base A (2/4);  total (34/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

CAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCGGCCTTC  >  NC_002947/1805975‑1806263                                                                                                                                               |                                                                                                                                                    cAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑Gc                                                                                                                                              <  3:404929/149‑1 (MQ=255)   aCCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGTTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtat                                                                                                                                            <  5:180198/149‑4 (MQ=37)   aCCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtat                                                                                                                                            <  8:68246/149‑4 (MQ=38)     cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatca                                                                                                                                          <  1:110171/149‑6 (MQ=37)     cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatc                                                                                                                                           >  2:110171/1‑144 (MQ=37)     cTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGCAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGAt                                                                                                                                          <  1:236738/149‑1 (MQ=255)     cTGCCCGGGCTGGTGCAGGCGGATTACCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatca                                                                                                                                          <  5:206314/149‑6 (MQ=37)     cTGCCCGGGCTGGTGCAGGCGGATTACCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTCCTGTGTTTTCGCGGAACCCACAGAAGTT‑gtatc                                                                                                                                           >  6:206314/1‑144 (MQ=37)        cccGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTAc                                                                                                                                       <  6:349699/149‑1 (MQ=255)         ccGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTAcc                                                                                                                                      <  1:215947/149‑1 (MQ=255)           gggCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCaa                                                                                                                                    <  2:226095/149‑1 (MQ=255)             gCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAg                                                                                                                                   >  2:4728/1‑148 (MQ=255)                  tGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGa                                                                                                                                                    <  4:53877/127‑1 (MQ=255)                  tGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGa                                                                                                                                                    >  3:53877/1‑127 (MQ=255)                     aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                          <  3:152917/149‑1 (MQ=255)                     aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                          <  5:241279/149‑1 (MQ=255)                     aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                          <  4:167257/149‑1 (MQ=255)                     aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                          >  7:331203/1‑149 (MQ=255)                     aGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGtttttt                                                                                                                          <  1:81083/149‑1 (MQ=255)                         ggATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGcc                                                                                                                       >  5:143020/1‑148 (MQ=255)                         ggATTGCCAACTGCACTGGCAGATACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGAATACCATCCATAATTGATACAAATTGTGAGGGTACCGAAAAAACACAAGAGTG‑GCCGATAACCAAGAAGttttttcac                                                                                                                       >  7:260821/1‑145 (MQ=255)                          gATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGcc                                                                                                                       >  2:131037/1‑147 (MQ=255)                                  aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTc                                                                                                             <  1:46801/149‑1 (MQ=255)                                  aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTc                                                                                                             <  2:354559/149‑1 (MQ=255)                                  aCTGCACTGGCAGTTACCGACGAACGCGCAAGGTATGAGCTGAAGGGTACATTCCGGTAGACTCCCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTCCCAAGCAGCTTTTTGCCGAGCTGATTc                                                                                                             <  2:280610/149‑1 (MQ=255)                                        cTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGc                                                                                                                                  >  4:319439/1‑122 (MQ=255)                                        cTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGc                                                                                                                                  <  3:319439/122‑1 (MQ=255)                                         tGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAAt                                                                                                      >  5:60607/1‑149 (MQ=255)                                           gCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGa                                                                                                    <  5:175229/149‑1 (MQ=255)                                                   cgacgaACGCGCAAGGTTTGAGCTCAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTACGCGAAAACACAGGAGTT‑GCCGATTAACAAGCAGTTTTTTGCCGAGATGATTCTGGAAATTACGatcta                                                                                             >  7:257863/1‑144 (MQ=255)                                                       gaACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGc                                                                                         >  7:202364/1‑148 (MQ=255)                                                             gcAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCg                                                                                  <  6:29251/149‑1 (MQ=255)                                                              cAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCgg                                                                                 >  3:317617/1‑149 (MQ=255)                                                               aaGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCggg                                                                                <  8:260821/149‑1 (MQ=255)                                                                aGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGt                                                                               <  5:346942/149‑1 (MQ=255)                                                                  gTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGta                                                                              >  6:265853/1‑148 (MQ=255)                                                                     tGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAc                                                                           <  6:60607/148‑1 (MQ=255)                                                                          tGAAGGGTACATTCCGGTAGACTACCGTGCCTTCGTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGc                                                                     >  1:11936/1‑149 (MQ=255)                                                                          tGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGAATACCCTGGc                                                                     >  1:141457/1‑149 (MQ=255)                                                                           gAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCt                                                                    >  3:205451/1‑149 (MQ=255)                                                                            aaGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑TCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCt                                                                    <  6:143020/148‑1 (MQ=255)                                                                                 tACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATg                                                                                                     <  1:44337/110‑1 (MQ=255)                                                                                 tACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATg                                                                                                     >  2:44337/1‑110 (MQ=255)                                                                                   cATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGTTAACACAGGAGTT‑GCCGAATACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGa                                                             <  1:131037/148‑1 (MQ=255)                                                                                    aTTCCGGTAGACTACCCTCCCTTCTTGATACAACATCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGATTTATGCCCAGCTGATTCTGGAAATGACGAGCGAACGCCATTCCGGGTATACCCTGCCAGTACAGAcc                                                           >  5:146847/1‑148 (MQ=255)                                                                                              aCTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGGCGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGtttt                                                 >  6:351096/1‑149 (MQ=255)                                                                                              aCTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGttt                                                  <  8:331203/148‑1 (MQ=255)                                                                                                tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAccc                                                                         <  5:307530/123‑1 (MQ=255)                                                                                                tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATAccc                                                                         >  6:307530/1‑123 (MQ=255)                                                                                                tACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                               >  6:58723/1‑149 (MQ=255)                                                                                                   cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGCCGCGGCAGTTTTTc                                               >  2:328322/1‑146 (MQ=255)                                                                                                   cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                               >  8:232708/1‑146 (MQ=255)                                                                                                   cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                               >  5:192848/1‑146 (MQ=255)                                                                                                   cctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTc                                               <  6:192848/146‑1 (MQ=255)                                                                                                    ctccctTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCgaga                                           <  1:4728/149‑1 (MQ=255)                                                                                                        cttcttGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTg                                                                                                           >  5:124745/1‑81 (MQ=255)                                                                                                        cttcttGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTg                                                                                                           <  6:124745/81‑1 (MQ=255)                                                                                                              gATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACtg                                    <  5:58723/146‑1 (MQ=255)                                                                                                               aTACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAt                                >  1:277740/1‑149 (MQ=255)                                                                                                               aTACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCAGGGTATACCATGGCTGTACAGACGAGGCAGTTTTTCGCTCTGCACTGTGAt                                >  4:297527/1‑149 (MQ=255)                                                                                                                tACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAtt                               >  4:47232/1‑149 (MQ=255)                                                                                                                tACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGAt                                <  5:265853/148‑1 (MQ=255)                                                                                                                 aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAg                                                                                                >  2:18526/1‑83 (MQ=255)                                                                                                                 aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAg                                                                                                <  1:18526/83‑1 (MQ=255)                                                                                                                 aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGAc                                                            <  4:92868/119‑1 (MQ=255)                                                                                                                 aCAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGAc                                                            >  3:92868/1‑119 (MQ=255)                                                                                                                  cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTTGGCCGATAACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGACCGAACCCCATCCCGGGTATACCATGGATTTACAGACCCGGCAGTTTTTCGAGATGCACTTTGAtt                               >  1:268217/1‑148 (MQ=255)                                                                                                                  cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTg                              <  5:31872/148‑1 (MQ=255)                                                                                                                  cAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGa                             >  1:6984/1‑149 (MQ=255)                                                                                                                    aCTTCTGTGGGTTCCGCGTAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACg                           <  2:268217/149‑1 (MQ=255)                                                                                                                     cTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGc                          <  2:1639/149‑1 (MQ=255)                                                                                                                      ttCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCa                         <  2:11936/149‑1 (MQ=255)                                                                                                                           tgGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAgg                       <  7:232708/146‑1 (MQ=255)                                                                                                                           tgGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAgg                       <  8:202364/146‑1 (MQ=255)                                                                                                                            gggTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGa                                                                                                 <  1:24164/71‑1 (MQ=255)                                                                                                                            gggTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGa                                                                                                 >  2:24164/1‑71 (MQ=255)                                                                                                                                 ccGCGAAAACACAGGAGTT‑GCCGATTACCAAGAAGTTTTTTGCCGAGCTGATTCTGGAAATGACTAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGTCGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCggg               <  8:257863/148‑1 (MQ=255)                                                                                                                                  cgcgAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGGCGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCgggg              <  5:351096/148‑1 (MQ=255)                                                                                                                                        aaCAAATGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACCGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCgg       <  3:297527/149‑1 (MQ=255)                                                                                                                                           acaGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCGGcct    <  4:205451/149‑1 (MQ=255)                                                                                                                                             aGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCGGccttc  <  3:47232/149‑1 (MQ=255)                                                                                                                                               gAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCGGcc     <  7:349209/144‑1 (MQ=255)                                                                                                                                               gAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATCCACTGTGATTGACGCAGGTGATCGGGGGCTTCGGcc     >  8:349209/1‑144 (MQ=255)                                                                                                                                               |                                                                                                                                                    CAACCTGCCCGGGCTGGTGCAGGCGGATTGCCAACTGCACTGGCAGTTACCGACGAACGCGCAAGGTTTGAGCTGAAGGGTACATTCCGGTAGACTACCCTCCCTTCTTGATACAACTTCTGTGGGTTCCGCGAAAACACAGGAGTT‑GCCGATTACCAAGCAGTTTTTTGCCGAGCTGATTCTGGAAATGACGAGCGAACGCCATGCCGGGTATACCCTGGCTGTACAGACGCGGCAGTTTTTCGAGATGCACTGTGATTGACGCAGGTGATCGGGGGCTTCGGCCTTC  >  NC_002947/1805975‑1806263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑