Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,859,126 T→C 6.3% intergenic (+46/+55) PP_1661 → / ← PP_1662 dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,859,1260TC 6.3% 234.3 / 4.6 79intergenic (+46/+55)PP_1661/PP_1662dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/37);  new base C (2/3);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

TTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGTTGAC  >  NC_002947/1858979‑1859272
                                                                                                                                                   |                                                                                                                                                   
ttGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCAttt                                                                                                                                                    >  2:36196/1‑149 (MQ=255)
    aGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTggg                                                                                                                                                >  2:373955/1‑149 (MQ=255)
     gCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTgggg                                                                                                                                               <  3:179229/149‑1 (MQ=255)
          gCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGc                                                                                                                                           >  3:211375/1‑148 (MQ=255)
            aTCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCa                                                                                                                                        >  8:298565/1‑149 (MQ=255)
                 tGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAg                                                                                                                                   <  1:373955/149‑1 (MQ=255)
                 tGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAg                                                                                                                                   >  5:383348/1‑149 (MQ=255)
                    cgacCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGcc                                                                                                                                 >  3:176361/1‑148 (MQ=255)
                     gacCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGcc                                                                                                                                 >  4:208094/1‑147 (MQ=255)
                       ccTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATTCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAgg                                                                                                                             >  2:294336/1‑149 (MQ=255)
                        cTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGc                                                                                                                            >  1:176027/1‑149 (MQ=255)
                        cTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGc                                                                                                                            >  1:38425/1‑149 (MQ=255)
                           gACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTgggg                                                                                                                                               <  5:10378/127‑1 (MQ=255)
                           gACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTgggg                                                                                                                                               >  6:10378/1‑127 (MQ=255)
                           gACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAg                                                                                                                         >  1:221102/1‑149 (MQ=255)
                                gCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAgg                                                                                                                    >  4:379203/1‑149 (MQ=255)
                                 cGTTACGTGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAggg                                                                                                                   <  5:405359/149‑1 (MQ=255)
                                 cGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAggg                                                                                                                   <  5:410986/149‑1 (MQ=255)
                                         ggCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTcgccg                                                                                                           <  2:221102/149‑1 (MQ=255)
                                         ggCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTcgccg                                                                                                           >  4:5045/1‑149 (MQ=255)
                                              gCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCATTg                                                                                                      >  7:190402/1‑149 (MQ=255)
                                                cTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCAAAATGCTGCGGCATTGCCGGAGCATCGTggg                                                                                                                                                <  5:88107/105‑1 (MQ=25)
                                                cTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCAAAATGCTGCGGCATTGCCGGAGCATCGTggg                                                                                                                                                >  6:88107/1‑105 (MQ=25)
                                                      gATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGt                                                                                             >  5:140559/1‑149 (MQ=255)
                                                           ccTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAgggg                                                                                                                  <  4:303671/124‑1 (MQ=255)
                                                           ccTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAgggg                                                                                                                  >  3:303671/1‑124 (MQ=255)
                                                           ccTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCAtt                                                                                         <  5:105715/148‑1 (MQ=255)
                                                           ccTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCAtt                                                                                         >  6:105715/1‑148 (MQ=255)
                                                           ccTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATtg                                                                                        >  2:268587/1‑149 (MQ=255)
                                                            cTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCATTGG‑GCAGGGTCATtgt                                                                                       <  8:190402/149‑1 (MQ=255)
                                                                     cTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGgcgcgc                                                                              <  6:383348/149‑1 (MQ=255)
                                                                       gTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTg                                                                            >  6:138395/1‑149 (MQ=255)
                                                                        gcGGTAGAGGTGCGTTTCGGGGCGGGGTAGGCCCTGTCATGGCAACCCCACGCTGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTgg                                                                           <  7:287735/148‑1 (MQ=255)
                                                                              gagGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑CCAGGGTCATTGTGTGGCGCGCTGGTTTTcc                                                                     <  1:36196/149‑1 (MQ=255)
                                                                                 gTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAgg                                                                  >  4:133963/1‑149 (MQ=255)
                                                                                 gTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAgg                                                                  >  2:327607/1‑149 (MQ=255)
                                                                                     gTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGATTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCtt                                                              <  5:171335/149‑1 (MQ=255)
                                                                                      tttCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTc                                                             <  3:5045/149‑1 (MQ=255)
                                                                                          ggggCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCA‑GGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCt                                                        <  2:38425/149‑1 (MQ=255)
                                                                                            ggCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCt                                                        >  4:273368/1‑148 (MQ=255)
                                                                                                gtgtAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGtt                                                                         <  7:372828/127‑1 (MQ=255)
                                                                                                gtgtAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAAcg                                                   >  3:398836/1‑149 (MQ=255)
                                                                                                   tAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAAcgcg                                                 <  3:379203/148‑1 (MQ=255)
                                                                                                   tAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCgg                                                >  6:138109/1‑149 (MQ=255)
                                                                                                     ggCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTcc                                                                     <  7:49277/126‑1 (MQ=255)
                                                                                                     ggCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTcc                                                                     >  8:49277/1‑126 (MQ=255)
                                                                                                      gCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTAt                                             >  3:245773/1‑149 (MQ=255)
                                                                                                       cccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTAtt                                            >  8:114041/1‑149 (MQ=255)
                                                                                                        ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCTTTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTg                                           <  4:211375/149‑1 (MQ=255)
                                                                                                        ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTg                                           <  8:8777/149‑1 (MQ=255)
                                                                                                        ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTg                                           <  4:398836/149‑1 (MQ=255)
                                                                                                        ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTg                                           <  2:176027/149‑1 (MQ=255)
                                                                                                           gTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGTGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGgtg                                        >  5:98626/1‑149 (MQ=255)
                                                                                                            tCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGgtgt                                       <  7:298565/149‑1 (MQ=255)
                                                                                                            tCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGgt                                         <  5:138395/147‑1 (MQ=255)
                                                                                                              tgagCAACCCCAAAATGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGc                                     >  3:28417/4‑149 (MQ=37)
                                                                                                               gagCAACCCCAAAATGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAg                                                          <  4:356054/125‑1 (MQ=25)
                                                                                                                ggCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTcc                                                                     >  1:147513/1‑115 (MQ=255)
                                                                                                                ggCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTcc                                                                     <  2:147513/115‑1 (MQ=255)
                                                                                                                  cAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGTTCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc                                  <  1:327607/149‑1 (MQ=255)
                                                                                                                       ccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTc                            <  4:245773/149‑1 (MQ=255)
                                                                                                                        cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAAt                                                                                                       >  4:100934/1‑74 (MQ=255)
                                                                                                                        cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAAt                                                                                                       <  3:100934/74‑1 (MQ=255)
                                                                                                                        cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAg                                                                   <  8:357800/109‑1 (MQ=255)
                                                                                                                        cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAg                                                                   >  7:357800/1‑109 (MQ=255)
                                                                                                                           gATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTa                        <  3:208094/149‑1 (MQ=255)
                                                                                                                               ctgcGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGa                    <  4:28417/146‑1 (MQ=255)
                                                                                                                                tCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTgg                                                                           <  3:289279/93‑1 (MQ=255)
                                                                                                                                tCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTgg                                                                           >  4:289279/1‑93 (MQ=255)
                                                                                                                                  cGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGaa                   <  1:294336/147‑1 (MQ=255)
                                                                                                                                  cGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACa                 >  2:107989/1‑149 (MQ=255)
                                                                                                                                    gCATTGCCGGAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATg               >  5:114081/1‑149 (MQ=255)
                                                                                                                                    gCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATg               >  5:81554/1‑149 (MQ=255)
                                                                                                                                          ccGCAGCATTTTGGCGTTGCTCACAGAGCCCAGGCGAGTCAGGGGTACGCCGCAATGG‑GCAGGGTCATTGTGTGGAGCGCTGGTTTTCCAGGTCTTCAAGCTGAACGCGGTATTGGTCTGCTTCGTGTTCGTTATGGAACATGCcgac          >  1:100390/1‑148 (MQ=255)
                                                                                                                                            ggagcaTTTTGGGGTTGCTCACTGAGCGCAGGCGAGTCAGGGGTTCGCCGCATTTG‑GCAGGGTCTTTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTGAACGCGGTTTTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAg       <  2:100390/147‑1 (MQ=255)
                                                                                                                                            gcagcaTTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCgg                                                <  3:261201/108‑1 (MQ=255)
                                                                                                                                            gcagcaTTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCgg                                                >  4:261201/1‑108 (MQ=255)
                                                                                                                                              agcaTTTTGGGGTTGCTCACTGAGCCCAGTCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGtt     <  3:133963/149‑1 (MQ=255)
                                                                                                                                                 aTTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGTTGa   <  5:138109/148‑1 (MQ=255)
                                                                                                                                                 aTTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGTTGAc  <  6:140559/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGG‑GCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGTTGAC  >  NC_002947/1858979‑1859272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑