Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,906,760 T→A 9.4% intergenic (+100/+28) nirD → / ← PP_1707 nitrite reductase/D‑isomer specific 2‑hydroxyacid dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,906,7600TA 9.4% 119.6 / 3.8 46intergenic (+100/+28)nirD/PP_1707nitrite reductase/D‑isomer specific 2‑hydroxyacid dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base T (21/20);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

TGGCCGGTGCGGTTGAAGGGGGAGGCGGTGGAGCTGGCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCGGTTGCAGGAC  >  NC_002947/1906613‑1906907
                                                                                                                                                   |                                                                                                                                                   
tgGCCGGTGCGGGTGAAGGGGGAGGCGGTGGAGCTGGCGGTCGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTg                                                                                                                                                    <  5:360707/149‑1 (MQ=255)
        gCGGTTGAAGGGGGAGGCGGTGGAGCTGGCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCCGTGGGAGCGGGCAAGCCcgctcc                                                                                                                                            <  6:256868/149‑4 (MQ=255)
             tGAAGGGGGAGGCGGTGGAGCTGGCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                       <  5:105719/149‑1 (MQ=255)
              gAAGGGGGAGGCGGTGGAGCTGGCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGccc                                                                                                                                                 >  7:232353/1‑138 (MQ=255)
              gAAGGGGGAGGCGGTGGAGCTGGCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGccc                                                                                                                                                 <  8:232353/138‑1 (MQ=255)
                     gAGGCGGTGGAGCTGGCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACaa                                                                                                                               <  7:358769/149‑1 (MQ=255)
                                   ggCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGcc                                                                                                                            <  8:101468/138‑1 (MQ=255)
                                   ggCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGcc                                                                                                                            >  7:101468/1‑138 (MQ=255)
                                      ggTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGAGCCGACCCTGTGGGAGCGGGCTCGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATaaccaa                                                                                                              <  2:314982/149‑1 (MQ=255)
                                               aTACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTgc                                                                                                       <  3:271463/147‑1 (MQ=255)
                                                tACCCTTTTGCTGCCTGTGCGGGCCTCTTCGCGGTCAAGCCCGCTCCTACGTAATCTGCACCGCCTTGTAATAGGGCGCCGACCCTGTGGGAGCGGGATTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTgcggc                                                                                                    <  5:368351/149‑1 (MQ=39)
                                                 aCCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTgcggc                                                                                                    <  3:257891/148‑1 (MQ=255)
                                                     cTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAACCCGCTCCTACGGAATCTGCACCGCCATGTAATAAGGCGCCGACCCTGTGGGAGCGGGCTTTCCCCCGAAGAGGCCAGCACAAGCCCACTAGATAAACAACCCCAGCCGCGTgcg                                                                                               >  2:119698/1‑149 (MQ=38)
                                                           tgcGTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCATACGGAATCTGCACCGCCTTTTAATCCGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGcc                                                                                                                                      <  8:19384/104‑1 (MQ=25)
                                                           tgcCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATATGGCCCGCCATGTAATAACGGGCCGCCCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGcc                                                                                                                                      >  7:19384/1‑104 (MQ=21)
                                                                 tgCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAac                                                                                     <  1:119698/147‑1 (MQ=255)
                                                                    cGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGT‑GGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATc                                                                                >  2:263747/1‑148 (MQ=255)
                                                                      gCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATATGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATcca                                                                              >  2:18830/1‑149 (MQ=255)
                                                                             cgcgGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCt                                                                       >  3:93769/1‑149 (MQ=255)
                                                                                gggCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGc                                                                    >  7:210678/1‑149 (MQ=255)
                                                                                  gCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGc                                                                    <  7:187383/147‑1 (MQ=255)
                                                                                  gCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGc                                                                    >  8:187383/1‑147 (MQ=255)
                                                                                    aaGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGccac                                                                 >  4:167563/1‑148 (MQ=255)
                                                                                       cccGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGccaccacc                                                             <  4:93769/149‑1 (MQ=255)
                                                                                            tCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGAGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCAtctc                                                        >  1:78520/1‑149 (MQ=255)
                                                                                                        tGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGAt                                                                                                                    <  7:185330/77‑1 (MQ=255)
                                                                                                        tGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGAt                                                                                                                    >  8:185330/1‑77 (MQ=255)
                                                                                                             cGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCAtctc                                                        >  2:80112/1‑132 (MQ=255)
                                                                                                             cGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCAtctc                                                        <  1:80112/132‑1 (MQ=255)
                                                                                                              gCCTTGTAATCCGGCGCCGCCCCTGGGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATCACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCgtgt                                      <  7:287620/149‑1 (MQ=255)
                                                                                                                cTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACAGATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCt                                    <  1:385911/149‑1 (MQ=255)
                                                                                                                     aaTACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTa                                 >  8:116206/1‑147 (MQ=255)
                                                                                                                      aTACGGCTCCGACCCTGTGGGAGCGGACTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGAGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAg                              >  6:199273/1‑149 (MQ=255)
                                                                                                                        aCGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTg                            >  2:216732/1‑149 (MQ=255)
                                                                                                                         cGGCGCCGACACTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTgg                           >  4:315955/1‑149 (MQ=255)
                                                                                                                          ggCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTgg                           >  7:247253/1‑148 (MQ=255)
                                                                                                                           gcgcCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCa                         <  1:216732/149‑1 (MQ=255)
                                                                                                                                   ccTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTg                      <  8:326089/144‑1 (MQ=255)
                                                                                                                                   ccTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTg                      >  7:326089/1‑144 (MQ=255)
                                                                                                                                   ccTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGgcgc                  >  4:79445/1‑148 (MQ=255)
                                                                                                                                   ccTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGgcgc                  >  3:119823/1‑148 (MQ=255)
                                                                                                                                        cttcgCGGGCAAGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTAGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCg            >  4:132333/5‑149 (MQ=255)
                                                                                                                                         ttcgCGGGCAAGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCg            >  5:287202/4‑148 (MQ=255)
                                                                                                                                         ggAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCgg           <  3:79445/149‑1 (MQ=255)
                                                                                                                                           cgCGGGCAAGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCg            <  6:287202/145‑1 (MQ=255)
                                                                                                                                               ggattGCCAGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGTCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCGGTTGCAg     <  8:41414/146‑1 (MQ=255)
                                                                                                                                               gacTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCGGTTGCAg     <  5:199273/147‑1 (MQ=255)
                                                                                                                                                  ttGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCGGTTGCAGGAc  >  3:162446/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGCCGGTGCGGTTGAAGGGGGAGGCGGTGGAGCTGGCGGTGGCCTGATACCCCTTTGCTGCCTGTGCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGGAATCTGCACCGCCTTGTAATACGGCGCCGACCCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCAGCACAAGCCGACTAGATAACCAACCCCTGCGGCGTGCGCCCGGCAAACACATCCACCAGGCTGGCCACCACCATCTCGCCCATGCGCGTGCGTGTCTGTACAGTGGCACTGGCGCGATGCGGTTGCAGGAC  >  NC_002947/1906613‑1906907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑