Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,921,549 T→A 11.6% intergenic (+24/+37) PP_1720 → / ← PP_1721 alcohol dehydrogenase/phosphoserine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,921,5490TA11.6% 129.5 / 9.1 52intergenic (+24/+37)PP_1720/PP_1721alcohol dehydrogenase/phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (28/18);  new base A (2/4);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01

CGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGT  >  NC_002947/1921403‑1921692
                                                                                                                                                  |                                                                                                                                               
cGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTa                                                                                                                                               <  3:219827/149‑1 (MQ=255)
      tCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCcgc                                                                                                                                          >  3:288261/1‑148 (MQ=255)
          cgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTAAACCCGCGAAgg                                                                                                                                     <  2:307823/149‑1 (MQ=255)
            cAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTAAACCCGCGAAgggg                                                                                                                                   <  5:98491/149‑1 (MQ=255)
                tGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCgg                                                                                                                               >  1:42107/1‑149 (MQ=255)
                   tCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTAccc                                                                                                                                            >  2:273056/1‑133 (MQ=255)
                   tCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTAccc                                                                                                                                            <  1:273056/133‑1 (MQ=255)
                                  tGGAGTTGGCCCAGGCGGCTTAGGCCTCGGTGGTCACAAACCTGGTGGGGGGCAAGGTGGTGTTGGTTAGCCCCCATAGCCGGGTGTACGCGGGGCTGGCCCCTGCGCGGGTTTACCCGCTATGGGGCCGGCACAGCCCATAAAAACCt                                                                                                             <  4:33487/149‑1 (MQ=255)
                                      gTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCGTAGCGGGGTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcag                                                                                                         >  5:273628/1‑149 (MQ=255)
                                            ccAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGGTTTCCCGCGAAGGGGCCGGCCCAGCCCATTAAAACCTTcagctcccg                                                                                                   >  7:140001/1‑147 (MQ=255)
                                             cAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcagctccagc                                                                                                  >  3:182833/1‑149 (MQ=255)
                                              aGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCgg                                                                                                                               >  3:258972/1‑119 (MQ=255)
                                                    cgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATTAAACCCTTCAGCTGCTGCTCagag                                                                                            >  4:59622/1‑148 (MQ=255)
                                                        tGCCGAGGTGGAGACCAACCCGGTGTGGGGAAAGCTTGTGATGTGTATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTGCGCGGGTTTACCCGCTAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCgg                                                                                        <  4:28662/148‑1 (MQ=255)
                                                        tGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAAc                                                                                                               >  7:194166/1‑125 (MQ=255)
                                                        tGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAAc                                                                                                               <  8:194166/125‑1 (MQ=255)
                                                         gCCGAGGTGGAGACCAACCCGGGGTCGGGCACGCTGGTGAGGGTGTTCGACCCTAGCCTGGTTTACGGGGGGCTGGCCCCTTCGCGGGTTTACCCGCTAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGc                                                                                       <  4:182833/148‑1 (MQ=255)
                                                         gCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTAGCGGGTTTACCAGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGATCCAGCTCAGATTCGGcc                                                                                      >  4:68193/1‑149 (MQ=255)
                                                           ctatcTGGAGACCAACCTGGTGTCGGGCAAGGTGGTTATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCTAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCa                                                                                     <  2:157659/144‑1 (MQ=255)
                                                           cGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCAc                                                                                    >  7:325452/1‑149 (MQ=255)
                                                           cGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCAc                                                                                    <  6:129063/149‑1 (MQ=255)
                                                                  gagaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCa                                                                                     >  1:157659/1‑141 (MQ=255)
                                                                  gagaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCCCCCATTGa                                                                             >  7:355479/1‑149 (MQ=255)
                                                                  gagaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTg                                                                              <  4:288261/148‑1 (MQ=255)
                                                                    gaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCATCGCGGGGTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCCGCTCAGAGTCGGCCCGCCAATGag                                                                            >  4:237571/1‑147 (MQ=255)
                                                                    gaCAAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTCGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGAt                                                                            >  4:344295/1‑148 (MQ=255)
                                                                         accaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCgg                                                                       >  2:338081/1‑148 (MQ=255)
                                                                               tgtCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCTCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTc                                                                >  3:160827/1‑149 (MQ=255)
                                                                                   gggCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTcgcg                                                             >  2:126732/1‑148 (MQ=255)
                                                                                   gggCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCCGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTcgcg                                                             >  1:32079/1‑148 (MQ=255)
                                                                                             gtgatggtgatCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGt                                                  >  3:404296/1‑149 (MQ=255)
                                                                                                    tgatCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGtt                                           <  8:50334/149‑1 (MQ=255)
                                                                                                    tgatCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGACCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCAATGATCTCGGCATGATCGCCCAACACTGCGTACGCGt                                            >  3:286019/1‑148 (MQ=255)
                                                                                                                 cgtGGTGTAAGCGGGGCGGGCCCCTTCGCGGGTTTACCCGCTAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCg                                               <  2:352704/130‑1 (MQ=255)
                                                                                                                   tGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCggg                                             <  8:378925/132‑1 (MQ=255)
                                                                                                                   tGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCggg                                             >  7:378925/1‑132 (MQ=255)
                                                                                                                      ggtAAGCGGGGGTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCACAACACTGCGTCCGGGTTCACCACATGCGGGTGATc                         <  8:355479/148‑1 (MQ=255)
                                                                                                                        tAAGCGGGGCTGGCCCCTTCGCTGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGGATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCa                       <  3:316125/149‑1 (MQ=255)
                                                                                                                        tAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCa                       <  2:42107/149‑1 (MQ=255)
                                                                                                                                  tGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAacac                                                       <  5:179687/107‑1 (MQ=255)
                                                                                                                                  tGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAacac                                                       >  6:179687/1‑107 (MQ=255)
                                                                                                                                    gCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATATCGGCATGCTAGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaacaac            >  2:207251/1‑148 (MQ=255)
                                                                                                                                      cccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCaacaacaac         >  3:181938/1‑149 (MQ=255)
                                                                                                                                      cccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCaacaacaac         >  7:357955/1‑149 (MQ=255)
                                                                                                                                      cccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCaacaacaac         >  4:207880/1‑149 (MQ=255)
                                                                                                                                      cccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCaacaacaac         >  5:265169/1‑149 (MQ=255)
                                                                                                                                        cTTCGCGGGTAAACCCGCGAAGGGGCCAGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACtctc                  <  4:124697/138‑1 (MQ=255)
                                                                                                                                        cTTCGCGGGTAAACCCGCGAAGGGGCCAGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACtctc                  >  3:124697/1‑138 (MQ=255)
                                                                                                                                         ttCGCGGGTAAACCCGCGAAGGGGC‑‑‑‑‑CAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCggg                              <  4:242210/120‑1 (MQ=38)
                                                                                                                                         ttCGCGGGTAAACCCGCGAAGGGGC‑‑‑‑‑CAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCggg                              >  3:242210/1‑120 (MQ=38)
                                                                                                                                           cgcgGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGc      <  4:160827/147‑1 (MQ=255)
                                                                                                                                             cgGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGt  <  3:344295/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGT  >  NC_002947/1921403‑1921692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑