Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,933,674 A→C 8.4% L27R (CTC→CGC)  minD ← MinC‑MinD‑MinE system ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,933,6740AC 8.4% 217.4 / 9.7 71L27R (CTC→CGC) minDMinC‑MinD‑MinE system ATPase
Reads supporting (aligned to +/- strand):  ref base A (32/33);  new base C (4/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGG  >  NC_002947/1933531‑1933820
                                                                                                                                               |                                                                                                                                                  
tCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCg                                                                                                                                               >  5:21835/1‑149 (MQ=255)
  aGGGCCTGTTGCAGGTTGGCTTCGCCTTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCGCGGccac                                                                                                                                              <  5:227335/148‑3 (MQ=255)
  aGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCgg                                                                                                                                              <  7:65185/148‑1 (MQ=255)
    ggCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTAc                                                                                                                                           <  6:405507/149‑1 (MQ=255)
      ccTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCa                                                                                                                                         <  7:285182/149‑1 (MQ=255)
          ttGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATgg                                                                                                                                     >  5:194094/1‑149 (MQ=255)
          ttGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATgg                                                                                                                                     <  3:346680/149‑1 (MQ=255)
             cAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATggcgg                                                                                                                                  <  8:59417/149‑1 (MQ=255)
               ggTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGgcggcg                                                                                                                                >  5:60993/1‑149 (MQ=255)
                   ggCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGgcgccg                                                                                                                             <  7:321489/148‑3 (MQ=255)
                    gCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTgg                                                                                                                            >  5:148222/1‑148 (MQ=255)
                    gCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATAACGGTCATGTGGCCGCGCAGTGCGAGGCCCGTACCAATGCCGCCCCTgg                                                                                                                            >  1:135422/1‑148 (MQ=255)
                      ttCGCCGTTGCCCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGAGCGAGGCCGGTACCAAAGGCGGCGCTGGTAg                                                                                                                         >  3:326223/1‑149 (MQ=255)
                         gCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                                       <  6:148222/148‑1 (MQ=255)
                         gCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                                       <  6:194094/148‑1 (MQ=255)
                           cGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTc                                                                                                                    >  3:136419/1‑149 (MQ=255)
                                 ccACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGTGGCGCTGGTa                                                                                                                          <  8:158797/137‑1 (MQ=255)
                                 ccACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGTGGCGCTGGTa                                                                                                                          >  7:158797/1‑137 (MQ=255)
                                 ccACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGccc                                                                                                              >  6:83178/1‑149 (MQ=255)
                                   aCGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATggcg                                                                                                                                   <  4:380819/126‑1 (MQ=255)
                                   aCGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATggcg                                                                                                                                   >  3:380819/1‑126 (MQ=255)
                                         aCGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACAccccc                                                                                                      >  4:94288/1‑149 (MQ=255)
                                          cGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGt                                                                                                                     >  6:79962/1‑133 (MQ=255)
                                          cGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGt                                                                                                                     <  5:79962/133‑1 (MQ=255)
                                          cGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACAcccccc                                                                                                     <  6:128113/149‑1 (MQ=255)
                                               tCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTTGTAGTGGTCTTGCCCACACACCCCTTGcc                                                                                                >  8:176155/1‑149 (MQ=255)
                                                 gTACACCACGCGGGGTTTGGAGGCCATGATAAGGTTGAGGTTACGCATGCCCAAGTGGTAGTCGTCAATGACGGTCTTGTGGCCGCGCATTTGGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCg                                                                                               <  2:135422/148‑1 (MQ=255)
                                                    caccacGCGGCGTTCGCAGCCCTTGATCAGGTCGAGGTTACGCAGGGCGACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGaa                                                                                            <  4:326223/148‑1 (MQ=255)
                                                         cgcgGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAAt                                                                                                                                       <  4:401891/100‑1 (MQ=255)
                                                         cgcgGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAAt                                                                                                                                       >  3:401891/1‑100 (MQ=255)
                                                         cgcgGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAAc                                                                                       >  5:312556/1‑148 (MQ=255)
                                                                ttCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGaa                                                                               >  6:334926/1‑149 (MQ=255)
                                                                   gCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCt                                                                            >  1:1395/1‑149 (MQ=255)
                                                                     aGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGGCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTg                                                                          <  6:21835/149‑1 (MQ=255)
                                                                      gCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTgg                                                                         <  6:60993/149‑1 (MQ=255)
                                                                              tCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGgtg                                                                 >  3:15244/1‑149 (MQ=255)
                                                                                 ggTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAAGGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCGGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGttt                                                              <  7:176155/149‑1 (MQ=255)
                                                                                   tCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCa                                                            <  2:1395/149‑1 (MQ=255)
                                                                                   tCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCa                                                            >  1:397057/1‑149 (MQ=255)
                                                                                   tCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCa                                                            >  7:238322/1‑149 (MQ=255)
                                                                                     gAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAcc                                                          >  5:266904/1‑149 (MQ=255)
                                                                                     gAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAcc                                                          >  3:136993/1‑149 (MQ=255)
                                                                                             gCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTaaaaa                                                  >  8:37219/1‑149 (MQ=255)
                                                                                             gCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTaaaaa                                                  >  7:172270/1‑149 (MQ=255)
                                                                                               aGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTaaaaa                                                  <  5:270997/147‑1 (MQ=255)
                                                                                               aGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTaaaaa                                                  >  6:270997/1‑147 (MQ=255)
                                                                                                  cccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTc                                             <  1:317594/149‑1 (MQ=255)
                                                                                                    cACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCgg                                           <  8:172270/149‑1 (MQ=255)
                                                                                                     aCGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCgg                                           >  8:126401/1‑148 (MQ=255)
                                                                                                       gTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGac                                        <  6:266904/149‑1 (MQ=255)
                                                                                                          gAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATa                                                >  5:114411/1‑138 (MQ=255)
                                                                                                          gAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATa                                                <  6:114411/138‑1 (MQ=255)
                                                                                                          gAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGacac                                      <  8:238322/148‑1 (MQ=255)
                                                                                                             gTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGt                                  <  3:94288/149‑1 (MQ=255)
                                                                                                             gTCGACAATGACGGTCTTGTGGCCGCGAAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTAGGCCAAGGTGTTTCCCCCCTAAATATAGTCGGCACACTTGt                                  >  6:275808/1‑149 (MQ=255)
                                                                                                                gACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGc                                                                        <  1:85731/108‑1 (MQ=255)
                                                                                                                gACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGc                                                                        >  2:85731/1‑108 (MQ=255)
                                                                                                                   aaTGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGc                            <  7:126401/149‑1 (MQ=255)
                                                                                                                         ggTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCaaaaaa                      <  5:83178/149‑1 (MQ=255)
                                                                                                                         ggTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCaaaaa                       >  7:353902/1‑148 (MQ=255)
                                                                                                                            gtaccggCCTCGCACTGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCt                              >  8:185282/6‑138 (MQ=255)
                                                                                                                            gtaccggCCTCGCACTGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCt                              <  7:185282/133‑1 (MQ=255)
                                                                                                                              accggCCTCGCACTGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGa                  >  2:270534/4‑148 (MQ=255)
                                                                                                                                tgGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGaaa                <  2:335208/148‑1 (MQ=255)
                                                                                                                                  gCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAg             <  4:136993/149‑1 (MQ=255)
                                                                                                                                   ccGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAccc                                                         <  6:392197/104‑1 (MQ=255)
                                                                                                                                   ccGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAccc                                                         >  5:392197/1‑104 (MQ=255)
                                                                                                                                         actGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCtaaat       >  7:240077/3‑148 (MQ=255)
                                                                                                                                         actGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCtaaat       >  5:395452/3‑148 (MQ=255)
                                                                                                                                             cGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAGATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAAAAAAg   <  4:15244/148‑1 (MQ=255)
                                                                                                                                              gAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAgg  >  2:303541/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAGG  >  NC_002947/1933531‑1933820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑