Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,948,173	C→G	7.1%	R494R (CGG→CGC)	PP_1749 ←	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,948,173	0	C	G	7.1%	256.6 / 7.3	81	R494R (CGG→CGC)	PP_1749	acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (33/42);  new base G (2/4);  total (35/46)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

TGCCCGGCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGAT  >  NC_002947/1948027‑1948316                                                                                                                                                    |                                                                                                                                                tGCCCGGCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGcgccg                                                                                                                                               >  6:310590/1‑149 (MQ=255)   cccGGCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCa                                                                                                                                             >  2:249145/1‑149 (MQ=255)   cccGGCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCa                                                                                                                                             >  6:321923/1‑149 (MQ=255)       gCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGc                                                                                                                                         <  1:169113/149‑1 (MQ=255)         aTCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGc                                                                                                                                         >  4:342531/1‑147 (MQ=255)            aCGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGGCGCACGGCCaacc                                                                                                                                    <  5:338613/149‑4 (MQ=255)             cGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGgcg                                                                                                                                   >  1:149693/1‑149 (MQ=255)             cGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGGGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGGCGCACGGCCaacct                                                                                                                                   <  3:244689/149‑5 (MQ=255)                  cATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggc                                                                                                                              >  5:354785/1‑149 (MQ=255)                  cATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggc                                                                                                                              >  2:53762/1‑149 (MQ=255)                   aTGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggcc                                                                                                                             <  4:131045/149‑1 (MQ=255)                    tGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccg                                                                                                                            >  1:398185/1‑149 (MQ=255)                    tGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccg                                                                                                                            >  4:111229/1‑149 (MQ=255)                        gTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGgccggccg                                                                                                                        <  1:53762/149‑1 (MQ=255)                        gTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGgccggccg                                                                                                                        <  8:161201/149‑1 (MQ=255)                        gTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGCGCCTggccgtgcg                                                                                                                        <  5:150316/149‑5 (MQ=255)                         tCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGccggccgg                                                                                                                       >  7:91445/1‑149 (MQ=255)                         tCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGccggccgg                                                                                                                       >  3:239290/1‑149 (MQ=255)                         tCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGGGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGGTGGCCGTGCGCCGCACGGCCAGGCGCTGGccggccgg                                                                                                                       <  5:274010/149‑1 (MQ=255)                         tCCAGCCCCACCACCGGAATTTCCACTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCCGGTCCAGGCGTTCGGTGACGTCCTCCACGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGccggccgg                                                                                                                       >  4:9070/1‑149 (MQ=255)                           cAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAgg                                                                                                                                     <  8:142026/133‑1 (MQ=255)                           cAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAgg                                                                                                                                     >  7:142026/1‑133 (MQ=255)                           cAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGGGCAGGCGTTCGGTGACGGCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCcggccggc                                                                                                                      <  3:223862/148‑1 (MQ=255)                                 caccacCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACAcgt                                                                                                               >  6:141842/1‑149 (MQ=255)                                         gAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAg                                                                                                       <  8:91445/149‑1 (MQ=255)                                         gAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAg                                                                                                       >  8:127393/1‑149 (MQ=255)                                         gAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAg                                                                                                       <  6:354785/149‑1 (MQ=255)                                           aTTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACTGCCAGGCGCTAGCCGGCCGGCAACACGTCGTCATAGc                                                                                                      <  4:116890/148‑1 (MQ=255)                                                 tgctgccGTG‑CGCGCGGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGcc                                                                                               >  4:65461/6‑149 (MQ=255)                                                 aGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGACGTCATAGCCAACGcc                                                                                               >  7:371409/1‑149 (MQ=255)                                                  gTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGccc                                                                                              >  7:144202/1‑149 (MQ=255)                                                   tGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCg                                                                                             <  3:342531/149‑1 (MQ=255)                                                            cgcgc‑gCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCa                                                                                    <  8:14776/149‑1 (MQ=255)                                                             gcgc‑gcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCaa                                                                                   <  5:321923/149‑1 (MQ=255)                                                             gcgc‑gcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCaa                                                                                   <  1:249145/149‑1 (MQ=255)                                                                c‑gcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGt                                                                                <  5:353746/149‑1 (MQ=255)                                                                c‑gcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGt                                                                                <  7:127393/149‑1 (MQ=255)                                                                  gcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTg                                                                               >  1:120950/1‑149 (MQ=255)                                                                    aCGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccggcc                                                                                                                         >  6:163112/1‑105 (MQ=255)                                                                    aCGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccggcc                                                                                                                         <  5:163112/105‑1 (MQ=255)                                                                    aCGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACATCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTgcg                                                                             <  7:335099/149‑1 (MQ=255)                                                                      ggCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCt                                                                           >  2:409666/1‑149 (MQ=255)                                                                       gcagcaTCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTc                                                                          <  2:136839/149‑1 (MQ=255)                                                                          gcaTCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCACGGTGACGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCgga                                                                       >  6:10409/1‑148 (MQ=255)                                                                            aTCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCTGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGttt                                                                     <  2:146137/149‑1 (MQ=255)                                                                             tCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTtc                                                                    <  1:409666/149‑1 (MQ=255)                                                                                gCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTtcgtc                                                                 <  8:144202/149‑1 (MQ=255)                                                                                   aGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCgtcgtc                                                              >  1:193390/1‑149 (MQ=255)                                                                                   aGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCgtcgtc                                                              <  2:149693/149‑1 (MQ=255)                                                                                       ccGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGc                                                          >  6:104642/1‑149 (MQ=255)                                                                                         gggTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCgg                                                        <  1:388822/149‑1 (MQ=255)                                                                                          ggTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGa                                                       <  6:32757/149‑1 (MQ=255)                                                                                           gTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGaa                                                      >  4:61659/1‑149 (MQ=255)                                                                                                 gCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGccc                                                                                              >  5:88182/1‑103 (MQ=255)                                                                                                 gCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGccc                                                                                              <  6:88182/103‑1 (MQ=255)                                                                                                 gCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCg                                                  <  3:206605/147‑1 (MQ=255)                                                                                                 gCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCg                                                  >  4:206605/1‑147 (MQ=255)                                                                                                               cctccAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTg                                  <  3:65461/149‑1 (MQ=255)                                                                                                                ctccAGTGTGCCGCGGGTGTGCAGGTTGGCCGTGCGGCGCACGGGCAGGCGCTGGCCGGACGGCAACACGTCGTCATAGCCAACGCCCGCTGCGAGCAAGGTGCGCTCTTTTTCTTCGTCCAGCGGAATGCGGCTCTCTCAACCGGTgg                                 <  8:272923/149‑1 (MQ=255)                                                                                                                ctccAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTgg                                 <  8:371409/149‑1 (MQ=255)                                                                                                                 tccAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTgg                                 >  7:454/1‑148 (MQ=255)                                                                                                                   cAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCg                                                                         <  3:82072/106‑1 (MQ=255)                                                                                                                   cAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCg                                                                         >  4:82072/1‑106 (MQ=255)                                                                                                                   cAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGa                                                       >  6:61334/1‑124 (MQ=255)                                                                                                                   cAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGa                                                       <  5:61334/124‑1 (MQ=255)                                                                                                                   cAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGc                                <  5:141842/147‑1 (MQ=255)                                                                                                                      ggTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCt                           <  3:61659/149‑1 (MQ=255)                                                                                                                           cgccgGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAg                                                           <  1:364127/112‑1 (MQ=255)                                                                                                                           cgccgGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAg                                                           >  2:364127/1‑112 (MQ=255)                                                                                                                               ggTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGAc                  <  2:193390/149‑1 (MQ=255)                                                                                                                                      aGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTg           <  2:120950/149‑1 (MQ=255)                                                                                                                                      aGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTg           <  4:97872/149‑1 (MQ=255)                                                                                                                                         ctGGCCGTGCGGCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGAc                  <  7:381631/138‑1 (MQ=255)                                                                                                                                         ctGGCCGTGCGGCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGAc                  >  8:381631/2‑139 (MQ=255)                                                                                                                                         ctGGCCGTGCGGCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGATGGCTCTgcgc        >  6:146124/2‑149 (MQ=255)                                                                                                                                            gCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCtt      <  3:9070/148‑1 (MQ=255)                                                                                                                                            gCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTc     >  3:34647/1‑149 (MQ=255)                                                                                                                                             ccGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTc     >  2:81812/1‑148 (MQ=255)                                                                                                                                             ccGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTc     >  6:305984/1‑148 (MQ=255)                                                                                                                                             ccGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCg    <  5:130459/149‑1 (MQ=255)                                                                                                                                               gTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGAt  <  1:81812/149‑1 (MQ=255)                                                                                                                                               gTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGAt  <  8:454/149‑1 (MQ=255)                                                                                                                                                   gccgcACGGCCAGGCGCTGGCCGGCCGGCAACCCGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGc                        <  2:154804/123‑1 (MQ=255)                                                                                                                                                   gccgcACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGc                        >  1:154804/1‑123 (MQ=255)                                                                                                                                                    |                                                                                                                                                TGCCCGGCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGC‑GCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGAT  >  NC_002947/1948027‑1948316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑