Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,977,236 C→G 5.4% R300R (CGC→CGG)  PP_1770 → 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,977,2360CG 5.4% 242.5 / 2.0 74R300R (CGC→CGG) PP_17703‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base G (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CGACGCTGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGTACTACCG  >  NC_002947/1977089‑1977384
                                                                                                                                                    |                                                                                                                                                    
cgACGCTGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCccgcc                                                                                                                                                     >  3:348046/1‑149 (MQ=255)
      tGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTAGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGGCGGGc                                                                                                                                                 >  1:173694/1‑147 (MQ=255)
      tGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTAGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGGCGGGc                                                                                                                                                 >  1:179871/1‑147 (MQ=255)
        tCCTGCGCACGCTTGATAGATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGGCGGGccag                                                                                                                                              <  8:80296/148‑3 (MQ=255)
          cTGCGCACGCTTGATACATTTCGGAGTGATCTCGACGCCCTGCGCGACGCTATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGGCGGGccaggat                                                                                                                                           <  8:119980/149‑6 (MQ=255)
          cTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTAtgt                                                                                                                                           >  5:310200/1‑149 (MQ=255)
             cgcACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGa                                                                                                                                        >  6:84063/1‑149 (MQ=255)
                    ttGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTAt                                                                                                                                  >  2:6117/1‑148 (MQ=255)
                       aTACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAAc                                                                                                                              >  8:181831/1‑149 (MQ=255)
                         aCATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGc                                                                                                                            >  2:23334/1‑149 (MQ=255)
                         aCATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACACGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAAccc                                                                                                                            >  7:97057/1‑147 (MQ=255)
                           aTTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAAc                                                                                                                              >  2:257991/1‑145 (MQ=255)
                           aTTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAAc                                                                                                                              <  1:257991/145‑1 (MQ=255)
                                      aTCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGc                                                                                                                                                 >  1:31563/1‑115 (MQ=255)
                                      aTCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGc                                                                                                                                                 <  2:31563/115‑1 (MQ=255)
                                        ctcGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGAACGGAttt                                                                                                              >  6:290988/1‑148 (MQ=255)
                                            aCGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAg                                                                                                         >  5:24548/1‑149 (MQ=255)
                                              gCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGc                                                                                                       >  4:11843/1‑149 (MQ=255)
                                                ccTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGcc                                                                                                      >  7:9573/1‑148 (MQ=255)
                                                 cTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGccc                                                                                                     >  6:299644/1‑148 (MQ=255)
                                                       gacgcgaTCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCggg                                                                                              >  3:252942/1‑149 (MQ=255)
                                                          gcgaTCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTgg                                                                                           >  7:257025/1‑149 (MQ=255)
                                                              tCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGgccgc                                                                                       <  5:65419/149‑1 (MQ=255)
                                                              tCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGgccgc                                                                                       <  3:11843/149‑1 (MQ=255)
                                                                gCTGAAGGGGATGGGCTCCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCt                                                                                     <  8:148400/149‑1 (MQ=255)
                                                                gCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCt                                                                                     <  8:9573/149‑1 (MQ=255)
                                                                    aaGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAAc                                                                                 >  2:95580/1‑149 (MQ=255)
                                                                      ggggATGGGCACCAGCTGCTGGGTGTATTCGCCCGCGCACGGGTTGCCCGCGAGCA‑TTTCATTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTCGCCGCCTGAACgg                                                                               >  5:196488/1‑149 (MQ=255)
                                                                        ggATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGc                                                                             <  2:99983/149‑1 (MQ=255)
                                                                        ggATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGc                                                                             <  4:182224/149‑1 (MQ=255)
                                                                             ggCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGc                                                                             <  1:367345/144‑1 (MQ=255)
                                                                             ggCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGc                                                                             >  2:367345/1‑144 (MQ=255)
                                                                             ggCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGAt                                                                         <  5:84063/148‑1 (MQ=255)
                                                                                aCCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCgt                                                                     <  1:23334/149‑1 (MQ=255)
                                                                                  cAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATc                                                                                                                    <  3:211698/100‑1 (MQ=255)
                                                                                  cAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATc                                                                                                                    >  4:211698/1‑100 (MQ=255)
                                                                                        ctgGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCggg                                                             >  8:155094/1‑149 (MQ=255)
                                                                                        ctgGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCggg                                                             <  7:181831/149‑1 (MQ=255)
                                                                                        ctgGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCg                                                               <  6:24548/147‑1 (MQ=255)
                                                                                          gggTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAgg                                                                                                        >  1:367543/1‑104 (MQ=255)
                                                                                          gggTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAgg                                                                                                        <  2:367543/104‑1 (MQ=255)
                                                                                           tgtgtATTCACGCGCGCACTGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCATATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGa                                                          <  8:97057/148‑1 (MQ=255)
                                                                                           ggTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCgtgt                                                                   >  5:59387/1‑140 (MQ=255)
                                                                                           ggTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCgtgt                                                                   <  6:59387/140‑1 (MQ=255)
                                                                                           ggTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGc                                                            <  5:299644/147‑1 (MQ=255)
                                                                                           ggTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGc                                                            <  4:348046/147‑1 (MQ=255)
                                                                                           ggTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGa                                                          <  7:15152/149‑1 (MQ=255)
                                                                                                 ttCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATcc                                                                       <  7:177298/130‑1 (MQ=255)
                                                                                                 ttCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATcc                                                                       >  8:177298/1‑130 (MQ=255)
                                                                                                    aCCCGCGCACGGGTTGCCCGCGAGCAGTTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGa                                                  >  1:4690/1‑149 (MQ=255)
                                                                                                    aCCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGa                                                  <  6:194982/148‑1 (MQ=255)
                                                                                                    aCCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGAt                                                 >  6:212583/1‑149 (MQ=255)
                                                                                                    aCCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGAt                                                 >  4:57232/1‑149 (MQ=255)
                                                                                                       cgcgCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTc                                              >  7:102776/1‑149 (MQ=255)
                                                                                                          gcACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGAtt                                                <  1:13098/144‑1 (MQ=255)
                                                                                                          gcACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGAtt                                                >  2:13098/1‑144 (MQ=255)
                                                                                                          gcACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACCCCAACGATCTGATTTTCTAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCa                                           <  6:310200/149‑1 (MQ=255)
                                                                                                           cACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCAt                                          <  5:212583/149‑1 (MQ=255)
                                                                                                            aCGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATc                                         >  2:393380/1‑149 (MQ=255)
                                                                                                                     ccGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACggg                                                                              >  7:163838/1‑103 (MQ=255)
                                                                                                                     ccGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACggg                                                                              <  8:163838/103‑1 (MQ=255)
                                                                                                                     ccGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATc                                 <  7:328536/148‑1 (MQ=255)
                                                                                                                     ccGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCa                                >  5:115840/1‑149 (MQ=255)
                                                                                                                     ccGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCa                                >  3:385813/1‑149 (MQ=255)
                                                                                                                          aGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATgctc                           <  6:115840/149‑1 (MQ=255)
                                                                                                                           gCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTGCCATCGCTCGATCATgctcg                          <  3:57232/149‑1 (MQ=255)
                                                                                                                           gCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATgctcg                          <  2:4690/149‑1 (MQ=255)
                                                                                                                                  cAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGc                    >  2:263667/1‑149 (MQ=255)
                                                                                                                                   aGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGc                    <  8:102776/148‑1 (MQ=255)
                                                                                                                                   aGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGc                    <  8:257025/148‑1 (MQ=255)
                                                                                                                                   aGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCt                   >  6:147022/1‑149 (MQ=255)
                                                                                                                                    gTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTg                  <  1:393380/149‑1 (MQ=255)
                                                                                                                                      aaaaTCCTGGCCCGCGGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCTACCGGGTGGGCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTGGATTTCCCATCTCTCGATCATGCTCGGCTCGCTgg                 <  6:271367/148‑1 (MQ=255)
                                                                                                                                             tGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAgg          >  1:342931/1‑148 (MQ=255)
                                                                                                                                               gCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGta        <  4:385813/148‑1 (MQ=255)
                                                                                                                                                 ccgccgGGCCTAGGGGGACGCTATGAACGCCACCGAGCTGAGTTTCCAGGCCCAACCGGGTGGCCGACTGAACGGGCGGATCCGGGGACCGGGCGACAAGTCGATTTCCCATCGCTCGAGCATGCTCGGCTCGCTg                  <  7:114363/136‑1 (MQ=255)
                                                                                                                                                    ccgGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGTACTACCg  <  7:155094/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGACGCTGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCA‑TTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGTACTACCG  >  NC_002947/1977089‑1977384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑