Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,978,708	C→G	7.1%	F45L (TTC→TTG)	cmk →	cytidylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,978,708	0	C	G	7.1%	226.9 / 6.6	70	F45L (TTC→TTG)	cmk	cytidylate kinase
Reads supporting (aligned to +/- strand):  ref base C (31/34);  new base G (3/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GAAGAGGGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCAGCAACGTCATCCG  >  NC_002947/1978560‑1978854                                                                                                                                                     |                                                                                                                                                   gaagaGGGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTc                                                                                                                                                    <  1:21639/149‑1 (MQ=255) gaagaGGGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTc                                                                                                                                                    <  6:355825/149‑1 (MQ=255) gaagaGGGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTc                                                                                                                                                    <  2:223360/149‑1 (MQ=255)   agacGGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCa                                                                                                                                                   <  4:256570/148‑1 (MQ=255)    gagGGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAAt                                                                                                                                                 <  7:213793/149‑1 (MQ=255)     agGGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATg                                                                                                                                                <  7:2610/149‑1 (MQ=255)       ggCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGc                                                                                                                                               <  7:39914/148‑1 (MQ=255)        gCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCa                                                                                                                                             <  7:141195/149‑1 (MQ=255)        gCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCa                                                                                                                                             <  6:256776/149‑1 (MQ=255)          aaGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTGAATGCCAGc                                                                                                                                           <  2:200723/149‑1 (MQ=255)          aaGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAg                                                                                                                                            >  8:104089/1‑148 (MQ=255)                       aaTGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTc                                                                                                                               <  4:21156/148‑1 (MQ=255)                            cACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTGAATGCCAGc                                                                                                                                           >  8:366379/1‑131 (MQ=255)                                 gTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCaa                                                                                                                    <  5:238551/149‑1 (MQ=255)                                  ttATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAAc                                                                                                                   <  6:149312/149‑1 (MQ=255)                                    aTCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACg                                                                                                                  <  5:196935/148‑1 (MQ=255)                                     tCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGa                                                                                                                 <  8:252985/148‑1 (MQ=255)                                      cACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCt                                                                                                                          >  7:75149/1‑138 (MQ=255)                                      cACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCt                                                                                                                          <  8:75149/138‑1 (MQ=255)                                        ccATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAagag                                                                                                             >  7:87294/1‑149 (MQ=255)                                         cATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCCTCGACCTGACCAACGAAGAgc                                                                                                            >  6:111882/1‑149 (MQ=255)                                                  aCCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGAcc                                                                                                    >  2:362399/1‑148 (MQ=255)                                                    cAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGTACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAg                                                                                                 >  3:361778/1‑149 (MQ=255)                                                       gCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCACTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCtt                                                                                              >  5:185437/1‑149 (MQ=255)                                                       gCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACGCCGGCGCGCGGGATCGCTTGCTGGCCGGCAAGGCCCTCACCGCCGGCGGCGCCCTGACGACCGTAGGGCTTCTGcccgcgctg                                                                                              >  4:313830/1‑143 (MQ=255)                                                               ggCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGAc                                                                                                                            <  8:173085/111‑1 (MQ=255)                                                               ggCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGAc                                                                                                                            >  7:173085/1‑111 (MQ=255)                                                                 cAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTgc                                                                                            >  6:34606/1‑141 (MQ=255)                                                                 cAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTgc                                                                                            <  5:34606/141‑1 (MQ=255)                                                                 cAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATc                                                                                    <  5:44416/149‑1 (MQ=255)                                                                  aaGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCTTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCt                                                                                   <  5:111882/149‑1 (MQ=255)                                                                  aaGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGa                                                                                                      <  8:189712/130‑1 (MQ=255)                                                                  aaGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGa                                                                                                      >  7:189712/1‑130 (MQ=255)                                                                    gggCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTgg                                                                                 <  7:365585/149‑1 (MQ=255)                                                                     ggCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGaa                                                                                                                <  7:172622/117‑1 (MQ=255)                                                                     ggCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGaa                                                                                                                >  8:172622/1‑117 (MQ=255)                                                                     ggCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGa                                                                                <  1:362399/149‑1 (MQ=255)                                                                          ggTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAg                                                                                                 <  1:63919/127‑1 (MQ=255)                                                                          ggTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAg                                                                                                 >  2:63919/1‑127 (MQ=255)                                                                              gCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGtt                                                                       <  7:104089/149‑1 (MQ=255)                                                                                       aTTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGc                                                              >  3:284642/1‑149 (MQ=255)                                                                                        ttGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGcc                                                             >  7:252793/1‑149 (MQ=255)                                                                                              gcgAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGccc                                                       <  4:284642/149‑1 (MQ=255)                                                                                                      ggctggAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGc                                                >  1:329517/1‑148 (MQ=255)                                                                                                       gctggAAGCTGCTGGACTCCGGCGCGCTGTATCGCGTGCGGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTa                                              <  3:313830/149‑1 (MQ=255)                                                                                                        ctggAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTac                                             <  6:172295/149‑1 (MQ=255)                                                                                                           gAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTacaa                                           >  8:354256/1‑148 (MQ=255)                                                                                                                 gatgGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGCCCAACGAAGAGCTGCTGACCAAGATTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGataa                                    >  3:80788/3‑147 (MQ=255)                                                                                                                   tgGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATc                                                                   >  3:383058/1‑116 (MQ=255)                                                                                                                   tgGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATc                                                                   <  4:383058/116‑1 (MQ=255)                                                                                                                   tgGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGatcatc                                  >  5:366808/1‑149 (MQ=255)                                                                                                                      aCTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCtt                               <  8:209319/149‑1 (MQ=255)                                                                                                                       cTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTg                              <  8:87294/149‑1 (MQ=255)                                                                                                                        tCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCtt                               >  2:77547/1‑147 (MQ=255)                                                                                                                          cGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAgg                           <  4:361778/149‑1 (MQ=255)                                                                                                                          cGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAgg                           >  8:255205/1‑149 (MQ=255)                                                                                                                              gcgcTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGa                       <  7:354256/149‑1 (MQ=255)                                                                                                                               cgcTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGCCCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAg                      >  5:267227/1‑149 (MQ=255)                                                                                                                               cgcTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAg                      <  7:255205/149‑1 (MQ=255)                                                                                                                                gcTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAgg                     >  7:142699/1‑149 (MQ=255)                                                                                                                                  tGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGatcatc                                  <  6:161911/134‑1 (MQ=255)                                                                                                                                  tGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGatcatc                                  >  5:161911/1‑134 (MQ=255)                                                                                                                                  tGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGAc                   >  4:65661/1‑149 (MQ=255)                                                                                                                                   gTATCGCTTACTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACg                  >  5:385930/1‑149 (MQ=255)                                                                                                                                     cgtggttGCTGGCATTGAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGCGCCCGGTAAGCTTCAACAGATCATACTTGAGGGTGAGGACGt                 >  4:326145/6‑148 (MQ=255)                                                                                                                                      tCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCa               <  6:185437/149‑1 (MQ=255)                                                                                                                                          ttGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCAGCaa           >  8:80697/1‑149 (MQ=255)                                                                                                                                           tGCTGGCATTGAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGt                                                                        >  3:7433/1‑87 (MQ=255)                                                                                                                                           tGCTGGCATTGAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGt                                                                        <  4:7433/87‑1 (MQ=255)                                                                                                                                                gCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAgg                           >  5:314477/1‑127 (MQ=255)                                                                                                                                                gCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAgg                           <  6:314477/127‑1 (MQ=255)                                                                                                                                                  aTTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCAGCAACGTCATcc   >  1:195174/1‑149 (MQ=255)                                                                                                                                                    tCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCAGCAACGTCATCCg  >  7:349303/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GAAGAGGGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCAGCAACGTCATCCG  >  NC_002947/1978560‑1978854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑