Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,029,411	G→T	100%	G384V (GGG→GTG)	wbpM →	polysaccharide biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,029,411	0	G	T	100.0%	215.8 / NA	65	G384V (GGG→GTG)	wbpM	polysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/33);  total (32/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGAGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGGGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGTTCGGCAC  >  NC_002947/2029265‑2029552                                                                                                                                                    |                                                                                                                                               cGAGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGgtgt                                                                                                                                              <  1:128161/149‑1 (MQ=255) cGAGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGgtgt                                                                                                                                              >  1:150139/1‑149 (MQ=255)   aGCTTTGCCGACAGATCATAGGGCGTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCAACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCg                                                                                                                                            <  8:388424/149‑1 (MQ=255)             ccAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTAGCAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAAt                                                                                                                                   >  1:313659/1‑149 (MQ=255)             ccAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAAt                                                                                                                                   >  1:400735/1‑148 (MQ=255)             ccAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAAt                                                                                                                                   >  4:152904/1‑148 (MQ=255)              cAGATCATAGGGCTCGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAAt                                                                                                                                   >  7:68585/1‑147 (MQ=255)                     tAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCAt                                                                                                                          >  6:275191/1‑149 (MQ=255)                                 cAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑t                                                                                                              >  8:280506/1‑148 (MQ=255)                                  aaCGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTa                                                                                                            <  2:150139/149‑1 (MQ=255)                                    cGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATg                                                                                                          <  8:195376/149‑1 (MQ=255)                                         ttGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCAc                                                                                                     <  8:68585/149‑1 (MQ=255)                                              gctgTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑t                                                                                                              >  7:107817/1‑135 (MQ=255)                                              gctgTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑t                                                                                                              <  8:107817/135‑1 (MQ=255)                                                 gTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCggg                                                                                             >  6:367382/1‑149 (MQ=255)                                                  ttCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCgg                                                                                              <  6:56489/147‑1 (MQ=255)                                                    cGACCAGAGCGAGTA‑CTCCCTCTACGCTATCCTCAGTGAGCTGTAGCAACGCGTTGCGCTCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGGGGTTa                                                                                           <  5:303291/148‑1 (MQ=255)                                                    cGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATg                                                                                                          <  1:226511/133‑1 (MQ=255)                                                    cGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATg                                                                                                          >  2:226511/1‑133 (MQ=255)                                                     gACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGc                                                                                         <  2:400735/149‑1 (MQ=255)                                                     gACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCAATTGGCGGGTAGc                                                                                         >  2:319461/1‑149 (MQ=255)                                                      aCCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGc                                                                                         >  7:259873/1‑148 (MQ=255)                                                         acaGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCAcc                                                                                     <  5:418069/149‑1 (MQ=255)                                                          caGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCg                                                                                    >  1:149312/1‑149 (MQ=255)                                                          caGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCg                                                                                    >  1:155422/1‑149 (MQ=255)                                                           aGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGt                                                                                   <  7:280506/149‑1 (MQ=255)                                                                    cAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCAcg                                                                           >  1:339322/1‑149 (MQ=255)                                                                        ctctACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACgcggc                                                                       >  1:28435/1‑149 (MQ=255)                                                                             cGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCt                                                                   <  3:31023/148‑1 (MQ=255)                                                                              gCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTa                                                                 <  3:152904/149‑1 (MQ=255)                                                                               cTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTAc                                                                <  2:339322/149‑1 (MQ=255)                                                                                  tCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACa                                                               <  1:297703/148‑1 (MQ=255)                                                                                   ccTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACaaa                                                             >  6:117420/1‑148 (MQ=255)                                                                                     tCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACAt                                                          >  1:296103/1‑149 (MQ=255)                                                                                          gAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGc                                                      <  1:319461/148‑1 (MQ=255)                                                                                             cTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCAt                                                  <  8:259873/149‑1 (MQ=255)                                                                                                gAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGcggcgg                                                                     >  4:396331/1‑127 (MQ=255)                                                                                                gAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGt                                               >  2:81029/1‑149 (MQ=255)                                                                                                gAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGt                                               >  7:101472/1‑149 (MQ=255)                                                                                                   cAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGtt                                              >  4:175836/1‑147 (MQ=255)                                                                                                   cAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGtt                                              >  1:203316/1‑147 (MQ=255)                                                                                                   cAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGa                                            <  3:175836/149‑1 (MQ=255)                                                                                                     aCGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAg                                           >  6:35321/1‑148 (MQ=255)                                                                                                         gTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGGGGCTTACAAACATGTGCCCATGGTTGAGcac                                        >  5:118365/1‑147 (MQ=255)                                                                                                             cgcgcgAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGcc                                                     >  4:377194/1‑130 (MQ=255)                                                                                                             cgcgcgAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGcc                                                     <  3:377194/130‑1 (MQ=255)                                                                                                              gcgcgAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATgg                                 <  2:313659/149‑1 (MQ=255)                                                                                                                   aGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAg                            <  5:35321/149‑1 (MQ=255)                                                                                                                    gTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAgg                           <  2:28435/149‑1 (MQ=255)                                                                                                                    gTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAgg                           <  5:117420/149‑1 (MQ=255)                                                                                                                      cGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGt                         <  2:149312/149‑1 (MQ=255)                                                                                                                       gCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGTCCATGGTTGAGCACAACATGGCGGAGGGt                         <  2:155422/148‑1 (MQ=255)                                                                                                                           ccGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATAc                     <  5:275191/148‑1 (MQ=255)                                                                                                                                  gACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCTATGGTTGAGCACAACATGGc                                <  5:48331/130‑1 (MQ=255)                                                                                                                                  gACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCTATGGTTGAGCACAACATGGc                                >  6:48331/1‑130 (MQ=255)                                                                                                                                       ctgcCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACAt                                                          <  3:39507/99‑1 (MQ=255)                                                                                                                                       ctgcCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACAt                                                          >  4:39507/1‑99 (MQ=255)                                                                                                                                       ctgcCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGtt        <  1:81029/149‑1 (MQ=255)                                                                                                                                          cccATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCTGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCacaaca                                    <  6:2387/118‑1 (MQ=255)                                                                                                                                          cccATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCacaaca                                    >  5:2387/1‑118 (MQ=255)                                                                                                                                          cccATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGTTc       <  7:216574/147‑1 (MQ=255)                                                                                                                                          cccATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGTTc       >  8:216574/1‑147 (MQ=255)                                                                                                                                          cccATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGTTc       <  2:203316/147‑1 (MQ=255)                                                                                                                                            cATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCtt                                                                  >  5:287658/1‑86 (MQ=255)                                                                                                                                            cATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCtt                                                                  <  6:287658/86‑1 (MQ=255)                                                                                                                                             aTTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGTTCGGCAc  >  8:312754/1‑149 (MQ=255)                                                                                                                                              ttCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGCCGGAGGGTATACTCAACAATGTGTTCGGCAc  >  6:289580/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                               CGAGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTA‑CAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGGGTCGGTACGCAATCAGCAGCATCTTGCCCACG‑TTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGTTCGGCAC  >  NC_002947/2029265‑2029552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑