| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,063,208 | G→A | 13.3% | intergenic (+65/‑142) | PP_1838 → / → PP_1839 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,063,208 | 0 | G | A | 13.3% | 152.8 / 11.7 | 60 | intergenic (+65/‑142) | PP_1838/PP_1839 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (24/28); new base A (5/3); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01 | |||||||||||
GAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAT > NC_002947/2063069‑2063351 | gAAGCGTATATCCAGACCTCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTtgtg < 5:55533/148‑1 (MQ=255)gAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTtgtg < 4:147390/148‑1 (MQ=255) gTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCaatgc > 3:281358/1‑145 (MQ=25) tatCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCaatgcct < 4:281358/148‑7 (MQ=21) atCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgt > 1:257664/1‑149 (MQ=255) atCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgt > 1:269086/1‑149 (MQ=255) cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGc > 7:375721/1‑148 (MQ=255) cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGc < 3:317187/148‑1 (MQ=255) cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCATGCCCCCGACCCCAGGCATTGCACAGGGTTCGGAAGATGTGGAGGTCCtgtggg > 4:32047/1‑147 (MQ=255) cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑gg < 8:341630/149‑1 (MQ=255) gcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑ggg < 5:127239/149‑1 (MQ=255) gACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGACCTGCACAAGGTCCGAACCCTGTGCaatgc < 8:201177/134‑5 (MQ=21) gACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGACCTGCACAAGGTCCGAACCCTGTGCaatgc > 7:201177/1‑130 (MQ=21) aaGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGcc < 3:32047/149‑1 (MQ=255) agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑gg < 1:65120/139‑1 (MQ=255) agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑gg > 2:65120/1‑139 (MQ=255) ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAAt < 8:375721/147‑1 (MQ=255) acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCa > 1:199934/1‑108 (MQ=255) acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCa < 2:199934/108‑1 (MQ=255) cgccgGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCGCCCACAGGCATTGCCCAGGGGTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCCAGCCCGCGCATGGGCCCCGCCCaa > 3:78841/1‑149 (MQ=38) tggtgCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGCACAGGGGTCGGACCTTGTGCAGGTCGTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCg < 8:220912/149‑1 (MQ=255) cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCCTTGCCCAGGGTTAGGACCTTTTGCAGGTACTGTGCGAGC‑GGGCAAGCCCGCGAAAGGGCCCCGCACAATTTTGCCGGGCt > 7:226444/1‑148 (MQ=37) cGGCACACCGAAGTAAACCGGGGCCGGGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGCACAGGGTTGGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGaa < 2:137058/149‑1 (MQ=255) cGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGcc < 6:83040/126‑1 (MQ=255) cGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGcc > 5:83040/1‑126 (MQ=255) gCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCCGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTGCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa < 1:122867/149‑1 (MQ=255) acCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGa > 7:364721/1‑97 (MQ=255) acCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGa < 8:364721/97‑1 (MQ=255) cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCt < 6:130064/89‑1 (MQ=255) cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCt > 5:130064/1‑89 (MQ=255) aaGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCGCTCCCACAGGCATTGCACAGGGTTAGGACCATTTGGAGGTGCGGTGCGAGC‑CGGCAAGCCCGCGAATGGGCCCCGCACAATTTTACCGGACTGAAAATTATgccc > 4:361909/1‑149 (MQ=37) ggggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTGCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGGCCCGCGCCATTTTGCCGGCCTGCACATTATGCCCGCCCCAAgg > 1:254057/1‑149 (MQ=37) cgcgTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGt < 2:82698/149‑1 (MQ=255) cGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCa < 2:257664/148‑1 (MQ=255) ggCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGCGC‑GGGCAAGCCCGCGAATGGGTCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAgg < 2:269086/149‑1 (MQ=255) acATTCGCGGGCTTGCCCGCTCCCACAGGCTTTGCACAGGGTTCGGACCTTGTGCTGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCAt < 4:78841/148‑1 (MQ=255) cgGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCacacac < 3:361909/149‑1 (MQ=255) gCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa > 8:290349/1‑102 (MQ=255) gCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa < 7:290349/102‑1 (MQ=255) ccGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGC‑GGGCAAGCCCGCGAATGGGCCGCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc > 6:138023/1‑149 (MQ=17) ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGTGC‑GGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc < 8:226444/149‑1 (MQ=255) ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATc < 1:56285/134‑1 (MQ=255) ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATc > 2:56285/1‑134 (MQ=255) ccGCTCCCACAGGCATTGCACAGGGTTAGGACCTTGTGCAGGACCTGTGCAAGC‑GGGCAAACCCGCGAAAGGGCCCCGCACAATTTTACCGGACTGAACATTATCCCCGACCCAAGGTCAGTTATTCAGGCATAACAAAACTGGTGttg > 7:61095/1‑148 (MQ=255) gCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc < 3:172627/147‑1 (MQ=18) gCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc > 4:172627/1‑147 (MQ=18) cacaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCTCTAATGGGCCCCGTACAATTTTCCCGTACTGAACATTATCCCCGTTTCAAGGTTAGTTATTCATGCATCATACACTTGGTGTTCGTTGACt < 8:61095/149‑1 (MQ=255) cacaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt > 6:424110/1‑149 (MQ=255) acaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTc > 7:162137/1‑119 (MQ=255) acaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTc < 8:162137/119‑1 (MQ=255) ggCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga > 4:215799/1‑147 (MQ=255) cATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAgg > 4:218255/1‑149 (MQ=255) ttGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGTGC‑GGGAAAGCCCGCGAATGGGCCCCGCTCAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGt < 2:254057/149‑1 (MQ=255) acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGt > 6:71852/1‑149 (MQ=255) acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGt > 8:260801/1‑149 (MQ=255) acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAACCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTAACCCCGCCCCAAGGTAAGTTATTCAGGCATCAAACACCTGGTGTTCGATGACTTAGAGGGTCCGt > 8:253288/1‑149 (MQ=255) aaggTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt > 1:274085/3‑149 (MQ=37) aGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCGTCACACACCTGGTGTTc < 6:337490/128‑1 (MQ=255) aGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCGTCACACACCTGGTGTTc > 5:337490/1‑128 (MQ=255) gTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAt < 5:424110/149‑1 (MQ=255) | GAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAT > NC_002947/2063069‑2063351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |