Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	325,135	T→C	6.1%	Y141H (TAC→CAC)	oprQ →	outer‑membrane porin D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	325,135	0	T	C	6.1%	191.7 / 2.3	65	Y141H (TAC→CAC)	oprQ	outer‑membrane porin D
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base C (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GCGGCAAAGGCCACAACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGC  >  NC_002947/324989‑325278                                                                                                                                                    |                                                                                                                                                gcggcAAAGGCCACAACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGTCTCCGCATTTCCAACACCGTGCTGAAGt                                                                                                                                                 <  1:220107/148‑1 (MQ=255)  cggcAAAGGCCACAACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCACATTTCCAACACCGTGCTGAAGTACg                                                                                                                                              <  4:116049/149‑1 (MQ=255)    gcAAAGGCCACAACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGt                                                                                                                                            >  2:52812/1‑149 (MQ=255)      aaaGGCCACAACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTACTTCAGCACGGTgt                                                                                                                                          <  1:156180/149‑2 (MQ=255)       aaGGCCACAACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTACTTCAGCACGGTgt                                                                                                                                          >  2:156180/1‑147 (MQ=255)        aGGCCACAACGGTGGTGGTGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGAc                                                                                                                                         >  6:254113/1‑148 (MQ=255)        aGGCCACAACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGAcc                                                                                                                                        <  8:274622/149‑1 (MQ=255)            cacaACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGAt                                                                                                                                    >  5:328257/1‑149 (MQ=255)             acaACGGTGGTGGCGGCGTCGCCTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGGTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATg                                                                                                                                   >  2:158290/1‑149 (MQ=255)                aCGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGCACGGTGACCAGATGCCa                                                                                                                                <  6:408463/149‑1 (MQ=255)                    tggtggCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCAc                                                                                                                            >  1:213912/1‑149 (MQ=255)                    tggtggCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGCTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCAc                                                                                                                            >  5:342209/1‑149 (MQ=255)                               tCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGGCAGtgctg                                                                                                                 <  4:187766/149‑1 (MQ=255)                                  aCTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGGCAGtgctggca                                                                                                              <  6:39332/149‑4 (MQ=255)                                  aCTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAg                                                                                                              >  3:221881/1‑149 (MQ=255)                                    ttcttcAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGt                                                                                                                                            >  4:270505/1‑117 (MQ=255)                                    ttcttcAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGt                                                                                                                                            <  3:270505/117‑1 (MQ=255)                                    ttcttcAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTa                                                                                                            >  3:177232/1‑149 (MQ=255)                                    ttcttcAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTa                                                                                                            >  6:238872/1‑149 (MQ=255)                                         cAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTacgac                                                                                                        >  5:287690/1‑148 (MQ=255)                                         cAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTacgac                                                                                                        >  5:59047/1‑148 (MQ=255)                                         cAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTacga                                                                                                         <  1:329599/147‑1 (MQ=255)                                          aaGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTAcgacga                                                                                                      >  3:56812/1‑149 (MQ=255)                                           aGCCGGACGAAACCACCAATGGCTAAGGCATCGCCAGCTCGCCGCAGAACCTGTCGCGTGGTGGTGCTGATGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGAt                                                                                                     <  2:312308/149‑1 (MQ=255)                                           aGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGAt                                                                                                     <  5:26411/149‑1 (MQ=255)                                           aGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGAt                                                                                                     <  5:254113/149‑1 (MQ=255)                                              cGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGtgctg                                                                                                                 >  8:202334/1‑134 (MQ=255)                                              cGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGtgctg                                                                                                                 <  7:202334/134‑1 (MQ=255)                                               gCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCa                                                                                                                       >  2:22986/1‑127 (MQ=255)                                               gCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCa                                                                                                                       <  1:22986/127‑1 (MQ=255)                                               gCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTacga                                                                                                         >  6:252587/1‑141 (MQ=255)                                               gCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTacga                                                                                                         <  5:252587/141‑1 (MQ=255)                                               gCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCAc                                                                                                 >  2:270444/1‑149 (MQ=255)                                               gCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCAc                                                                                                 <  1:270444/149‑1 (MQ=255)                                                      accaccAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTctgct                                                                                          <  1:52812/149‑1 (MQ=255)                                                      accaccAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTctgct                                                                                          <  5:238872/149‑1 (MQ=255)                                                       ccaccaATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTctgctg                                                                                         <  2:105507/149‑1 (MQ=255)                                                        caccaATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCtgctgc                                                                                        <  4:56812/149‑1 (MQ=255)                                                              tGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGaaa                                                                                  <  5:208679/149‑1 (MQ=255)                                                                           gCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCAc                                                                     >  7:156642/1‑149 (MQ=255)                                                                                cTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGa                                                                >  8:344358/1‑149 (MQ=255)                                                                                   gccgcACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGAt                                                               <  8:156642/147‑1 (MQ=255)                                                                                      gcACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCAcc                                                                    >  3:216246/1‑139 (MQ=255)                                                                                      gcACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCAcc                                                                    <  4:216246/139‑1 (MQ=255)                                                                                       cacaACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGAt                                                                                                                                    >  2:245507/1‑74 (MQ=255)                                                                                                gcgcGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAagag                                                  <  4:221881/147‑1 (MQ=255)                                                                                                 cgcgTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATc                                               <  6:287690/149‑1 (MQ=255)                                                                                                  gcgTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATc                                               >  5:216945/1‑148 (MQ=255)                                                                                                                      aGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACgcc                          >  5:358601/1‑149 (MQ=255)                                                                                                                       agatCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACgcc                          >  6:32725/4‑149 (MQ=255)                                                                                                                        t‑tCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCaaa                                                     >  6:299910/1‑120 (MQ=255)                                                                                                                        t‑tCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCaaa                                                     <  5:299910/120‑1 (MQ=255)                                                                                                                           ccGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTg                                      <  3:302403/133‑1 (MQ=255)                                                                                                                           ccGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTg                                      >  4:302403/1‑133 (MQ=255)                                                                                                                             gCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGTCCGc                    >  4:27908/1‑149 (MQ=255)                                                                                                                             gCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGc                    <  4:154910/149‑1 (MQ=255)                                                                                                                             gCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGc                    <  6:328257/149‑1 (MQ=255)                                                                                                                             gCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGc                    >  3:390574/1‑149 (MQ=255)                                                                                                                                   ccAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCAc               <  6:15098/148‑1 (MQ=255)                                                                                                                                    ggtcacCGTACTTCAGCACGGTGACCAGATGCCGGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCAcc              >  2:71471/4‑148 (MQ=255)                                                                                                                                     aaCACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCAcc              >  7:232795/1‑147 (MQ=255)                                                                                                                                      acacCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAg           <  8:162510/149‑1 (MQ=255)                                                                                                                                              cTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAgcgc    <  7:344358/148‑1 (MQ=255)                                                                                                                                              cTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAgcgc    <  4:390574/148‑1 (MQ=255)                                                                                                                                               tGAAGTACGGTGACCAGATGCCAGCACTGCCAGTCCAGCAGTACGACCATGCACCACTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGACATCCACGGCCAGGAACTGACCGCCGGCCCCTTCACCCAGGAAgcccgc  >  6:34841/1‑149 (MQ=255)                                                                                                                                                gAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAgcgcgc  >  5:352068/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                GCGGCAAAGGCCACAACGGTGGTGGCGGCGTCGACTTCTTCAAGCCGCACGAAACCACCAATGCCGAAGGCAACGCCAGCTCGCCGCACAACCTGGCGCGTGGTGGTGCTGCTGTGAAGT‑TCCGCATTTCCAACACCGTGCTGAAGTACGGTGACCAGATGCCAGCACTGCCAGTGCTGCAGTACGACGATGCACGTCTGCTGCCAGAAAGCTTCACCGGCACCCTGATCACCTCCAAAGAGATCCAGGGCCTGGAACTGAACGCCGGCCGCTTCACCCAGGAAGCGCGC  >  NC_002947/324989‑325278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑