Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,063,228 C→G 9.4% intergenic (+85/‑122) PP_1838 → / → PP_1839 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,063,2280CG 9.4% 152.8 / 7.0 53intergenic (+85/‑122)PP_1838/PP_1839membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base G (3/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

CAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAGTCA  >  NC_002947/2063081‑2063368
                                                                                                                                                   |                                                                                                                                             
cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGc                                                                                                                                               >  7:375721/1‑148 (MQ=255)
cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGc                                                                                                                                               <  3:317187/148‑1 (MQ=255)
     cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑gg                                                                                                                                        <  8:341630/149‑1 (MQ=255)
      gcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑ggg                                                                                                                                       <  5:127239/149‑1 (MQ=255)
            aaGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGcc                                                                                                                                 <  3:32047/149‑1 (MQ=255)
               agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑gg                                                                                                                                        <  1:65120/139‑1 (MQ=255)
               agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑gg                                                                                                                                        >  2:65120/1‑139 (MQ=255)
                     ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAAt                                                                                                                          <  8:375721/147‑1 (MQ=255)
                                cgccgGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCGCCCACAGGCATTGCCCAGGGGTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCCAGCCCGCGCATGGGCCCCGCCCaa                                                                                                             >  3:78841/1‑149 (MQ=38)
                                        tggtgCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGCACAGGGGTCGGACCTTGTGCAGGTCGTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCg                                                                                                     <  8:220912/149‑1 (MQ=255)
                                             cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCCTTGCCCAGGGTTAGGACCTTTTGCAGGTACTGTGCGAGC‑GGGCAAGCCCGCGAAAGGGCCCCGCACAATTTTGCCGGGCt                                                                                                 >  7:226444/1‑148 (MQ=37)
                                               cGGCACACCGAAGTAAACCGGGGCCGGGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGCACAGGGTTGGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGaa                                                                                              <  2:137058/149‑1 (MQ=255)
                                               cGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGcc                                                                                                                     <  6:83040/126‑1 (MQ=255)
                                               cGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGcc                                                                                                                     >  5:83040/1‑126 (MQ=255)
                                                 gCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCCGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTGCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa                                                                                            <  1:122867/149‑1 (MQ=255)
                                                     acCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGa                                                                                                                                             <  8:364721/97‑1 (MQ=255)
                                                     acCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGa                                                                                                                                             >  7:364721/1‑97 (MQ=255)
                                                         aaGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCGCTCCCACAGGCATTGCACAGGGTTAGGACCATTTGGAGGTGCGGTGCGAGC‑CGGCAAGCCCGCGAATGGGCCCCGCACAATTTTACCGGACTGAAAATTATgccc                                                                                    >  4:361909/1‑149 (MQ=37)
                                                                  ggggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTGCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGGCCCGCGCCATTTTGCCGGCCTGCACATTATGCCCGCCCCAAgg                                                                           >  1:254057/1‑149 (MQ=37)
                                                                       cgcgTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGt                                                                      <  2:82698/149‑1 (MQ=255)
                                                                              cGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCa                                                                <  2:257664/148‑1 (MQ=255)
                                                                               ggCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGCGC‑GGGCAAGCCCGCGAATGGGTCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAgg                                                              <  2:269086/149‑1 (MQ=255)
                                                                                  acATTCGCGGGCTTGCCCGCTCCCACAGGCTTTGCACAGGGTTCGGACCTTGTGCTGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCAt                                                           <  4:78841/148‑1 (MQ=255)
                                                                                         cgGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCacacac                                                    <  3:361909/149‑1 (MQ=255)
                                                                                                gCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa                                                                                            >  8:290349/1‑102 (MQ=255)
                                                                                                gCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa                                                                                            <  7:290349/102‑1 (MQ=255)
                                                                                                  ccGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGC‑GGGCAAGCCCGCGAATGGGCCGCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc                                           >  6:138023/1‑149 (MQ=17)
                                                                                                  ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGTGC‑GGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc                                           <  8:226444/149‑1 (MQ=255)
                                                                                                  ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATc                                                          <  1:56285/134‑1 (MQ=255)
                                                                                                  ccGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATc                                                          >  2:56285/1‑134 (MQ=255)
                                                                                                  ccGCTCCCACAGGCATTGCACAGGGTTAGGACCTTGTGCAGGACCTGTGCAAGC‑GGGCAAACCCGCGAAAGGGCCCCGCACAATTTTACCGGACTGAACATTATCCCCGACCCAAGGTCAGTTATTCAGGCATAACAAAACTGGTGttg                                           >  7:61095/1‑148 (MQ=255)
                                                                                                    gCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc                                           <  3:172627/147‑1 (MQ=18)
                                                                                                    gCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc                                           >  4:172627/1‑147 (MQ=18)
                                                                                                         cacaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCTCTAATGGGCCCCGTACAATTTTCCCGTACTGAACATTATCCCCGTTTCAAGGTTAGTTATTCATGCATCATACACTTGGTGTTCGTTGACt                                    <  8:61095/149‑1 (MQ=255)
                                                                                                         cacaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt                                    >  6:424110/1‑149 (MQ=255)
                                                                                                          acaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTc                                                                 >  7:162137/1‑119 (MQ=255)
                                                                                                          acaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTc                                                                 <  8:162137/119‑1 (MQ=255)
                                                                                                             ggCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga                                  >  4:215799/1‑147 (MQ=255)
                                                                                                               cATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAgg                              >  4:218255/1‑149 (MQ=255)
                                                                                                                 ttGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGTGC‑GGGAAAGCCCGCGAATGGGCCCCGCTCAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGt                            <  2:254057/149‑1 (MQ=255)
                                                                                                                     acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGt                        >  6:71852/1‑149 (MQ=255)
                                                                                                                     acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGt                        >  8:260801/1‑149 (MQ=255)
                                                                                                                     acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAACCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTAACCCCGCCCCAAGGTAAGTTATTCAGGCATCAAACACCTGGTGTTCGATGACTTAGAGGGTCCGt                        >  8:253288/1‑149 (MQ=255)
                                                                                                                       aaggTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt                       >  1:274085/3‑149 (MQ=37)
                                                                                                                       aGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCGTCACACACCTGGTGTTc                                           >  5:337490/1‑128 (MQ=255)
                                                                                                                       aGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCGTCACACACCTGGTGTTc                                           <  6:337490/128‑1 (MQ=255)
                                                                                                                          gTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAt                   <  5:424110/149‑1 (MQ=255)
                                                                                                                              gaaCCCTGTGCAATGCCTGTGGGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGaaa               <  2:274085/147‑1 (MQ=38)
                                                                                                                                     gtgCAGGTCCTGTGCGAGC‑GGGCAAGCGCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTg          <  7:253288/147‑1 (MQ=255)
                                                                                                                                        cAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATGCAGACATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAg     >  7:84665/1‑149 (MQ=255)
                                                                                                                                           gTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAt                   >  5:184462/1‑132 (MQ=255)
                                                                                                                                           gTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAt                   <  6:184462/132‑1 (MQ=255)
                                                                                                                                           gTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAGTCa  >  6:199079/1‑149 (MQ=255)
                                                                                                                                            tCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGaa      >  4:81656/1‑144 (MQ=255)
                                                                                                                                            tCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGaa      <  3:81656/144‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGC‑GGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAAGTCA  >  NC_002947/2063081‑2063368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑