Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,073,374 G→T 9.1% L12M (CTG→ATG) ‡ PP_1847 ← TonB‑dependent ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,073,3740GT 9.1% 132.8 / 4.4 44L12M (CTG→ATG) ‡PP_1847TonB‑dependent ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (21/19);  new base T (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

TGGCGCGGGCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGAGCGG  >  NC_002947/2073228‑2073514
                                                                                                                                                  |                                                                                                                                            
tGGCGCGGGCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGc                                                                                                                                            <  5:425701/149‑1 (MQ=255)
        gCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTAGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGc                                                                                                                                    >  8:197066/1‑149 (MQ=255)
          tGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCCTGCTCAgcctg                                                                                                                                    <  1:47091/147‑5 (MQ=255)
          tGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCCTGCTCAgcctg                                                                                                                                    >  2:47091/1‑143 (MQ=255)
            gAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAgg                                                                                                                                 >  4:325497/1‑148 (MQ=255)
            gAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAgg                                                                                                                                 <  3:325497/148‑1 (MQ=255)
             aaGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCCTGCTCAgcctgg                                                                                                                                   >  1:242222/1‑140 (MQ=255)
             aaGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCCTGCTCAgcctgg                                                                                                                                   <  2:242222/145‑6 (MQ=255)
                    ccACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGa                                                                                                                        <  2:77246/149‑1 (MQ=255)
                    ccACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGCGTGa                                                                                                                        >  3:375590/1‑149 (MQ=255)
                          cGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCCGTCTTCGGCAAGCACAGAGGGGCACGGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCggg                                                                                                                  <  7:192795/149‑1 (MQ=255)
                            gCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGACGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCggggg                                                                                                                >  1:309918/1‑149 (MQ=255)
                                    tGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGt                                                                                                        >  1:147580/1‑149 (MQ=255)
                                             gCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCgggg                                                                                                                            <  7:128534/120‑1 (MQ=255)
                                             gCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCgggg                                                                                                                            >  8:128534/1‑120 (MQ=255)
                                                atcatTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGa                                                                                            >  4:383606/1‑149 (MQ=255)
                                                  catTGGGGTTGGTGCCCGAGCCCTGAAATGGGCCCCTCTCCACGCTGTCTTCGGCCCCCACTGCGGGCCACTGATGGGCCGGGCCCTGGGGGTGCAGGCTCGGGGCCTGGCGGGGGTCATGGGGGGGCATGCGGTGGGGCTCCTGGAt                                                                                           <  4:196476/148‑1 (MQ=255)
                                                  catCGGCGTAGGGGCCTGCTACCTGCTCTGGGCCCATCCCCACGCCGCATTGGCCACCCACAGCGGGCGCCTGCTGGGCCTGGCCCTGGCTTAGCAGGCTCGGGTCCTGGCGGGGGTCATGGGGGGGCATGCGGTGGGGCTCCTGGAt                                                                                           <  4:190311/148‑1 (MQ=255)
                                                       gCGTAGGTGCCGGAGACCTGAAATGGGGCCAGCATCACGGTGTCTTCGGCAAGCACAGAGGGCCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTTATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGa                                                                                     <  2:309918/149‑1 (MQ=255)
                                                          tAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTAAGGGGCAGGCGGGGGTGATGCGGGGGCATGAGGTGGGGCTCCTGGATGCGCGATAc                                                                                  >  4:363080/1‑149 (MQ=255)
                                                               gtCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGACCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGTCATGCGGTGGGGCTCCTGGATGCGCGATACCTGt                                                                              >  5:102393/1‑148 (MQ=255)
                                                                tCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTAc                                                                            >  4:167871/1‑149 (MQ=255)
                                                                 cGGAGCCCTGCTATGGGCCCACCATCCCCGTGGGTTCGGCACGAACAGCGGGGCCCTGCTGGGCCAGCCCCCGGGTGTGCAGGCTCCGGGGCAGGCGGGGGTGCGGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACt                                                                           <  4:375590/149‑1 (MQ=255)
                                                                  ggAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTg                                                                          >  2:322098/1‑149 (MQ=255)
                                                                         tGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCttt                                                                   >  5:124653/1‑149 (MQ=255)
                                                                           cAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCgggg                                                                                                                 >  4:352526/1‑101 (MQ=255)
                                                                           cAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCgggg                                                                                                                 <  3:352526/101‑1 (MQ=255)
                                                                               ggggCCATCAGCCCGGTGTTTTCGGCAAGCACATCGGGGCCCTGCAGGCCACGGCCCAGGCTGAGCTGCCTCTGGGGCCGGCGGGGGTGTTGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCgg                                                              <  4:276836/148‑1 (MQ=255)
                                                                                       cATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCa                                                     >  2:225455/1‑149 (MQ=255)
                                                                                         tcccGCTGTCTTCGCCAACCACAGCGGGCCACGGCCGGGCCCGCCCCAGGCTCCGCAGGCCCAGGGGCAGGCGGGGGGCATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACt                                                   <  3:383606/146‑1 (MQ=255)
                                                                                         tCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCCCt                                                   >  3:304956/1‑149 (MQ=255)
                                                                                          cACGCTGGCTTCGGCACCCACCCAGGGCCACTGCCGGGCCAGGCCCCGGCCGCGCAGGCTCAGGGGCCGGCGGGGGTCAGGCGGGGGCATGCGGGGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTc                                                  <  1:322098/149‑1 (MQ=255)
                                                                                                            cactGAGGGGCACGGCCGGGCCTGGCCCAGGCTGAGCTGGCGCAGGGGCTGGCGGGGGTGAGGGGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATa                                <  4:304956/149‑1 (MQ=255)
                                                                                                                   gggCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATacac                             >  7:359259/1‑145 (MQ=255)
                                                                                                                      cACTGCAGGGCCAGGCCCAGGCTGAGCCGGCGCCGGGGCAGGCGGGGGGGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATgg                      <  6:124653/149‑1 (MQ=255)
                                                                                                                        cTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCCGGTGGTGATACACCAGATGGt                     >  5:50981/1‑148 (MQ=255)
                                                                                                                           ttgggCCCGCCCCTGGCGGCGCTGGCTCCGGGGCTGGGGGGGGTGAGGCGGGGGCAGGCGGGGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGa                   <  8:335676/145‑1 (MQ=255)
                                                                                                                             gggCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGa                   >  7:335676/1‑145 (MQ=255)
                                                                                                                             gggCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATccc               >  2:263837/1‑149 (MQ=255)
                                                                                                                              ggcacggccaaggcTTAGCTGGATCCGGGGCAGGCGGGTGTTTTCCGGGGGCATGCGGTGGGGCTCCTGTATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATcccc              <  6:102393/144‑1 (MQ=255)
                                                                                                                              ggCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACGCACTGCGAACCCGGGTGGTGTTACCACAGATGGTGATcccc              >  5:355246/1‑149 (MQ=255)
                                                                                                                                      cctggcTGAGCTGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGa      <  7:197066/146‑1 (MQ=255)
                                                                                                                                         ggcTGAGCAGGCTCAGGGGCAGGCGCGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGCTACCTGTACTGGCCCTTTCGCCGGTAAACCCACTCCTACAACGGGAGGTGATACACCAGATGGTGATCCCCCAGTAGAAGCg   >  6:422091/1‑149 (MQ=255)
                                                                                                                                          gcTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGAGCgg  <  3:167871/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TGGCGCGGGCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGAGCGG  >  NC_002947/2073228‑2073514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑