Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,098,218 A→C 5.8% L558V (TTG→GTG)  PP_1875 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,098,2180AC 5.8% 217.6 / 3.6 69L558V (TTG→GTG) PP_1875two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base A (35/30);  new base C (2/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GGTCACCAGCAGAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTAAAACAGCTGCCAAAGCCCGGCTCGGAGCGAATG  >  NC_002947/2098072‑2098366
                                                                                                                                                   |                                                                                                                                                    
ggTCACCAGCAGAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGt                                                                                                                                                    >  5:367529/1‑149 (MQ=255)
 gTCACCAGCAGAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGtg                                                                                                                                                   >  3:187/1‑149 (MQ=255)
  tCACCAGCAGAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGtgc                                                                                                                                                  <  7:185067/149‑1 (MQ=255)
    aCCAGCAGAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGtgctg                                                                                                                                                >  2:329285/1‑149 (MQ=255)
    aCCAGCAGAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATAGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGtgct                                                                                                                                                 <  2:330434/148‑1 (MQ=255)
     ccAGCAGAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCGCAGGCGGGCAGCAAGtgctgc                                                                                                                                               <  4:187/149‑1 (MQ=255)
      cagcagAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGtgctgc                                                                                                                                               >  7:169492/1‑148 (MQ=255)
      cagcagAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCAGGTAGGTCAGGCCCCAGGCGGGCAGCAAGtgctgc                                                                                                                                               >  3:315629/1‑148 (MQ=255)
       agcagAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCACTtgctgccc                                                                                                                                             <  7:318368/149‑3 (MQ=255)
       agcagAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCaa                                                                                                                                             >  7:331671/1‑149 (MQ=255)
           gAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGt                                                                                                                                         >  8:8830/1‑149 (MQ=255)
              tCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACa                                                                                                                                           >  4:321092/1‑144 (MQ=255)
              tCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACa                                                                                                                                           <  3:321092/144‑1 (MQ=255)
                 gggTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCa                                                                                                                                      >  6:232474/1‑146 (MQ=255)
                 gggTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCa                                                                                                                                      <  5:232474/146‑1 (MQ=255)
                       tCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGTCCCTGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGcc                                                                                                                               <  1:117369/148‑1 (MQ=255)
                         aaTGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCt                                                                                                                           >  7:50966/1‑149 (MQ=255)
                                ggCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCt                                                                                                                    <  8:331671/149‑1 (MQ=255)
                                 gcgcAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTg                                                                                                                   >  2:27831/1‑149 (MQ=255)
                                     aGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCACTtgctgcc                                                                                                                                              <  2:341201/118‑2 (MQ=255)
                                     aGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCACTtgctgcc                                                                                                                                              >  1:341201/1‑117 (MQ=255)
                                          gAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCAtt                                                                                                                                    <  4:237847/123‑1 (MQ=255)
                                          gAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCAtt                                                                                                                                    >  3:237847/1‑123 (MQ=255)
                                          gAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGt                                                                                                           <  5:86902/148‑1 (MQ=255)
                                           aaCAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACCGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGtg                                                                                                                                                   <  2:804/107‑1 (MQ=255)
                                           aaCAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACCGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGtg                                                                                                                                                   >  1:804/1‑107 (MQ=255)
                                               ggTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCa                                                                                                                                      >  2:346029/1‑116 (MQ=255)
                                                  gCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCAcg                                                                                                  >  6:415495/1‑149 (MQ=255)
                                                  gCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCAcg                                                                                                  >  4:174089/1‑149 (MQ=255)
                                                   cTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCAcgc                                                                                                 >  1:399825/1‑149 (MQ=255)
                                                    tCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCAcgcg                                                                                                >  7:242002/1‑149 (MQ=255)
                                                      gACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGc                                                                                              >  7:150686/1‑149 (MQ=255)
                                                          tcgtcgGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGcc                                                                                             >  5:65313/1‑146 (MQ=255)
                                                          tcgtcgGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGcc                                                                                             <  6:65313/146‑1 (MQ=255)
                                                            gtcgGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGc                                                                                         >  2:211518/1‑148 (MQ=255)
                                                               ggTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAgg                                                                                     <  8:348588/149‑1 (MQ=255)
                                                               ggTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAgg                                                                                     <  8:150686/149‑1 (MQ=255)
                                                                gTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAg                                                                                                                                 >  4:224754/1‑104 (MQ=255)
                                                                gTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAggg                                                                                    >  3:129963/1‑149 (MQ=255)
                                                                 tGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCAt                                                                                           <  8:370883/141‑1 (MQ=255)
                                                                 tGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCAt                                                                                           >  7:370883/1‑141 (MQ=255)
                                                                 tGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGgc                                                                                   <  7:8830/149‑1 (MQ=255)
                                                                      aaCAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTgg                                                                              >  3:136219/1‑149 (MQ=255)
                                                                        cAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCg                                                                            <  2:399825/149‑1 (MQ=255)
                                                                         aGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGc                                                                                                                                >  6:63705/1‑96 (MQ=255)
                                                                         aGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGc                                                                                                                                <  5:63705/96‑1 (MQ=255)
                                                                           gTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGa                                                                         >  1:164659/1‑149 (MQ=255)
                                                                            tCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGAt                                                                        <  8:242002/149‑1 (MQ=255)
                                                                            tCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGAt                                                                        <  2:164659/149‑1 (MQ=255)
                                                                                      cGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCCGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGgt                                                               <  6:294975/148‑1 (MQ=255)
                                                                                      cGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGgtg                                                              >  3:235305/1‑149 (MQ=255)
                                                                                      cGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGgt                                                               <  4:129963/148‑1 (MQ=255)
                                                                                       ggCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGgtgt                                                             <  3:174089/149‑1 (MQ=255)
                                                                                         cAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGgtg                                                              <  1:92523/146‑1 (MQ=255)
                                                                                         cAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGgtg                                                              >  2:92523/1‑146 (MQ=255)
                                                                                               gCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTc                                                     <  8:169492/149‑1 (MQ=255)
                                                                                                  tGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGc                                                  >  7:367666/1‑149 (MQ=255)
                                                                                                        aaCTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGa                                                                         >  4:280453/1‑120 (MQ=255)
                                                                                                        aaCTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGa                                                                         <  3:280453/120‑1 (MQ=255)
                                                                                                             t‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTcggc                                       <  4:136219/149‑1 (MQ=255)
                                                                                                                aTGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTcggcg                                      <  2:60985/148‑1 (MQ=255)
                                                                                                                aTGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTcggcg                                      >  1:60985/1‑148 (MQ=255)
                                                                                                                 tGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGt                                    >  4:65347/1‑149 (MQ=255)
                                                                                                                     gcTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTaaaa                                <  4:235305/149‑1 (MQ=255)
                                                                                                                      cTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTAAAAc                               <  5:415495/149‑1 (MQ=255)
                                                                                                                                     ccAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTAAAACAGCTGCCAAAGCCCg                >  2:109551/1‑149 (MQ=255)
                                                                                                                                       actgttgcagcaCTTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTAAAACAGCTGCCAAAGCCCGGc              >  6:4351/7‑149 (MQ=255)
                                                                                                                                         gCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTAAAACAGCTGCCAAAGCCCGGCTc            >  1:43818/1‑149 (MQ=255)
                                                                                                                                                gcaAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTAAAACAGCTGCCAAAGCCCGGCTCGGAGCGa     <  1:27831/149‑1 (MQ=255)
                                                                                                                                                   aGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGCGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTAAAACAGCTGCCAAAGCCCGGCTCGGAGCGAAtg  >  6:27906/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGTCACCAGCAGAATCGGGGTTTTCAATGCCGGGCGCAGTTCGAACAGGTGCTCGACGTCGTCGGTGATCAACAGGTCGATCACTGCGGCAACCAGCGTGGCGCAACTGT‑CATGGCGCTGGTAGGTCAGGCCCCAGGCGGGCAGCAAGTGCTGCAACAGTTCATTGAGCCCGCTGCCGGCTGCGCTCAGTGCGGCCACGCGGCCATGCAAGGGCGCTGGCGGGATGGCTTCGGTGTGCACGGTCAGCGGCAATTCGGCGGTAAAACAGCTGCCAAAGCCCGGCTCGGAGCGAATG  >  NC_002947/2098072‑2098366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑