Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,147,208 T→G 10.4% intergenic (+81/+36) murB → / ← rne UDP‑N‑acetylenolpyruvoylglucosamine reductase/ribonuclease E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,147,2080TG10.4% 226.7 / 17.4 77intergenic (+81/+36)murB/rneUDP‑N‑acetylenolpyruvoylglucosamine reductase/ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (35/34);  new base G (4/4);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

GATTCAGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGA  >  NC_002947/2147064‑2147355
                                                                                                                                                |                                                                                                                                                   
gATTCAGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCa                                                                                                                                                 >  6:347443/1‑149 (MQ=255)
       gCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgg                                                                                                                                          <  8:235390/149‑1 (MQ=255)
        cGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCggg                                                                                                                                         <  4:321270/149‑1 (MQ=255)
           atatCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCt                                                                                                                                      >  6:223875/1‑149 (MQ=255)
            tatCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCtt                                                                                                                                     >  1:92582/1‑149 (MQ=255)
                ttCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCggg                                                                                                                                         <  7:31265/141‑1 (MQ=255)
                ttCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCggg                                                                                                                                         >  8:31265/1‑141 (MQ=255)
                  cGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCtttt                                                                                                                                   <  2:92582/145‑1 (MQ=255)
                     gCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTttcattca                                                                                                                            <  7:104410/149‑1 (MQ=255)
                         tttGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCtt                                                                                                                        <  5:223875/149‑1 (MQ=255)
                           tGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCttt                                                                                                                       <  7:30975/148‑1 (MQ=255)
                                         agaTGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGtga                                                                                                        >  8:341405/1‑149 (MQ=255)
                                               aGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGt                                                                                                  <  2:355114/149‑1 (MQ=255)
                                                 ccTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGttt                                                                                                >  1:295168/1‑149 (MQ=255)
                                                   tAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGgtgtgt                                                                                              >  4:319352/1‑149 (MQ=255)
                                                   tAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgt                                                                                              >  5:293018/1‑149 (MQ=255)
                                                   tAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgg                                                                                                                                          >  8:82714/1‑105 (MQ=38)
                                                   tAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgg                                                                                                                                          <  7:82714/105‑1 (MQ=38)
                                                     aTCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgtg                                                                                             <  4:311589/148‑1 (MQ=255)
                                                     aTCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgt                                                                                              <  2:327903/147‑1 (MQ=255)
                                                     aTCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCAGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgtg                                                                                             <  5:292648/148‑1 (MQ=255)
                                                     aTCTTTACTGATTTGCCGGCTGTGAACACCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGATATCAGACGTGATGAGGTTTGTGc                                                                                            >  7:258585/1‑149 (MQ=255)
                                                      tCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCt                                                                                           >  6:383041/1‑149 (MQ=255)
                                                        tttACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCa                                                                                         >  2:312303/1‑149 (MQ=255)
                                                              gATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgg                                                                                                                                          >  2:367457/1‑94 (MQ=37)
                                                              gATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgg                                                                                                                                          <  1:367457/94‑1 (MQ=37)
                                                               aTTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATcggcgg                                                                                  >  4:318665/1‑149 (MQ=255)
                                                                tttGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCggcggc                                                                                 >  3:114693/1‑149 (MQ=255)
                                                                    ccGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTc                                                                              <  2:234128/148‑1 (MQ=255)
                                                                     cGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTc                                                                              >  5:270035/1‑147 (MQ=255)
                                                                         tgtgAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGc                                                                        >  2:49726/1‑149 (MQ=255)
                                                                            gAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTgg                                                                     >  6:359391/1‑149 (MQ=255)
                                                                            gAACCCCCGTAGGAGCGGGATTACCCGCTAAGAGGCCCGTCAGGAAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTg                                                                      <  8:258585/148‑1 (MQ=255)
                                                                             aaCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGt                                                                    <  1:49726/149‑1 (MQ=255)
                                                                                ccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                       <  4:278353/143‑1 (MQ=255)
                                                                                ccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                       >  3:278353/1‑143 (MQ=255)
                                                                                  ccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGttt                                                                                                >  6:417049/1‑116 (MQ=255)
                                                                                  ccGTAGGAGCGGGTTTACCCCCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGttt                                                                                                <  5:417049/116‑1 (MQ=255)
                                                                                    gTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTggg                                                             <  6:270035/149‑1 (MQ=255)
                                                                                        gAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGc                                                         <  1:179347/149‑1 (MQ=255)
                                                                                          gCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTg                                                       <  5:359391/149‑1 (MQ=255)
                                                                                             ggTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGCTCCTGGGAAGCTgcag                                                    >  4:154807/1‑149 (MQ=255)
                                                                                              gTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcagc                                                   >  5:283787/1‑149 (MQ=255)
                                                                                              gTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcag                                                    >  5:276654/1‑148 (MQ=255)
                                                                                                   cccGCGAAGAGGCCCTTCAGGAAAACCAAAAAAAGCCCCGCCTGTTTGCACTGGCGGGGCCTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCt                                               <  5:347443/148‑1 (MQ=255)
                                                                                                   cccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCtt                                              >  7:83387/1‑149 (MQ=255)
                                                                                                    ccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                             >  8:362174/1‑149 (MQ=255)
                                                                                                    ccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                             >  1:306460/1‑149 (MQ=255)
                                                                                                    ccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                             >  6:87661/1‑149 (MQ=255)
                                                                                                    ccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                             >  6:93517/1‑149 (MQ=255)
                                                                                                      gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATTGGCGGCTTCCAGGGCTGGTTCCTg                                                               <  2:57071/129‑1 (MQ=255)
                                                                                                      gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATTGGCGGCTTCCAGGGCTGGTTCCTg                                                               >  1:57071/1‑129 (MQ=255)
                                                                                                      gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                             <  4:347860/147‑1 (MQ=255)
                                                                                                      gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                             >  3:347860/1‑147 (MQ=255)
                                                                                                      gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCt                                           >  2:261586/1‑149 (MQ=255)
                                                                                                       cgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTACATTCAGCTTTGGCTATCAGACGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtg                                          >  1:114219/1‑149 (MQ=255)
                                                                                                          agagGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTg                                                                      >  4:255113/1‑118 (MQ=255)
                                                                                                          agagGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTg                                                                      <  3:255113/118‑1 (MQ=255)
                                                                                                               cccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCtt                                  <  5:93517/149‑1 (MQ=255)
                                                                                                               cccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCtt                                  <  5:87661/149‑1 (MQ=255)
                                                                                                                 cGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTg                                <  8:83387/149‑1 (MQ=255)
                                                                                                                 cGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTg                                <  1:312303/149‑1 (MQ=255)
                                                                                                                 cGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTg                                <  3:319352/149‑1 (MQ=255)
                                                                                                                  gTCAGGCAAACCAAAAAAAGCCCCGCGAGTTTGCACTGGCGGGTATTTTTCATTCAGCTTTGTCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCGAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTgg                               <  2:295168/149‑1 (MQ=255)
                                                                                                                        cAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGcagcag                         <  4:114693/149‑1 (MQ=255)
                                                                                                                          aaCCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgtg                                                                                             >  8:305334/1‑79 (MQ=255)
                                                                                                                          aaCCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgtg                                                                                             <  7:305334/79‑1 (MQ=255)
                                                                                                                             cAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                    >  3:211127/1‑149 (MQ=255)
                                                                                                                             cAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                    >  6:14262/1‑149 (MQ=255)
                                                                                                                               aaaaaaGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                       >  2:397090/1‑96 (MQ=255)
                                                                                                                               aaaaaaGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                       <  1:397090/96‑1 (MQ=255)
                                                                                                                                   aaGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTcc              <  2:306460/149‑1 (MQ=255)
                                                                                                                                       cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCTGCAGCTTccca                                          >  2:105443/1‑115 (MQ=39)
                                                                                                                                       cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCTGCAGCTTccca                                          <  1:105443/117‑3 (MQ=39)
                                                                                                                                       cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCt          >  2:109172/1‑149 (MQ=255)
                                                                                                                                          gCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTgc       <  5:14262/149‑1 (MQ=255)
                                                                                                                                           ccAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTgcg      <  6:276654/149‑1 (MQ=255)
                                                                                                                                               tttGCACTGGCGGGGCTTTTTCATTCAGCTTTCGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGa  <  6:293018/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GATTCAGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGA  >  NC_002947/2147064‑2147355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑