Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,215,833 A→T 8.2% intergenic (+647/‑297) PP_1957 → / → PP_5493 Pdr/VanB family oxidoreductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,215,8330AT 8.2% 178.6 / 5.8 61intergenic (+647/‑297)PP_1957/PP_5493Pdr/VanB family oxidoreductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/31);  new base T (2/3);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

TACGGAATGGGGTCAAACTGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTCCCCGTCACCGCCCCCTTGTCCCGA  >  NC_002947/2215687‑2215980
                                                                                                                                                  |                                                                                                                                                   
tACGGAATGGGGTCAAACTGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGaa                                                                                                                                                    <  8:10795/148‑1 (MQ=255)
      aTGGGGTCAAACTGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCTTCGCAAgga                                                                                                                                             <  3:168264/149‑2 (MQ=255)
         gggTCAAACTGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGtat                                                                                                                                          <  8:362716/149‑1 (MQ=255)
               aaCTGAATTTTCCACAAAAAATTTCATATTTTTCATTGACTTACTATTGACAAAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTg                                                                                                                                     >  1:284805/1‑148 (MQ=255)
                aCTGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTGAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGta                                                                                                                                   <  4:188734/149‑1 (MQ=255)
                 cTGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGtat                                                                                                                                  <  6:367061/149‑1 (MQ=255)
                  tGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCTTCGCAAggaag                                                                                                                                           <  1:13746/139‑4 (MQ=255)
                  tGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCTTCGCAAggaag                                                                                                                                           >  2:13746/1‑136 (MQ=255)
                  tGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGtat                                                                                                                                  >  6:245487/1‑148 (MQ=255)
                    aaTTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATAc                                                                                                                                <  5:372249/148‑1 (MQ=255)
                            tCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCTTCGCAAggaa                                                                                                                                            <  8:159895/128‑3 (MQ=255)
                            tCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCTTCGCAAggaa                                                                                                                                            >  7:159895/1‑126 (MQ=255)
                            tCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTatat                                                                                                                                        >  4:80250/1‑132 (MQ=255)
                            tCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGaaaa                                                                                                                       >  3:226851/1‑149 (MQ=255)
                              aaaaaaTTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAAcg                                                                                                                     >  2:87429/1‑149 (MQ=255)
                               aaaaaTTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAAcgc                                                                                                                    <  5:245487/149‑1 (MQ=255)
                                aaaaTTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAAcgcg                                                                                                                   >  7:182273/1‑149 (MQ=255)
                                             tttCATTGACTTAGTATTGACATAATTTATATAATATTTTTGATCTAAAGGCTTGCGCCTGGGTCAAGGGTGTTGCTCTACTACTTGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGaaaaaa                                                                                                       <  5:294950/148‑1 (MQ=255)
                                             tttCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGaaaaaa                                                                                                       <  2:61481/148‑1 (MQ=255)
                                                 aTTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAattgcg                                                                                                  <  1:87429/149‑1 (MQ=255)
                                                          cTATTGACAAAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCgcct                                                                                         <  2:284805/149‑1 (MQ=255)
                                                                aCATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCtt                                                                                    <  8:275229/148‑1 (MQ=255)
                                                                 cATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGa                                                                                  <  3:157282/149‑1 (MQ=255)
                                                                  attaTTTATATAATATTTATGATCTCAAGGCTGGCGCCTGGTGCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAg                                                                                 <  2:140997/146‑1 (MQ=255)
                                                                  aTAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAg                                                                                 >  7:329833/1‑149 (MQ=255)
                                                                    aaTTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGtt                                                                               >  1:246830/1‑149 (MQ=255)
                                                                         atataATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCg                                                                           <  8:182273/148‑1 (MQ=255)
                                                                         atataATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCg                                                                           <  1:248165/148‑1 (MQ=255)
                                                                             aataTTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGccc                                                                       >  6:321564/1‑148 (MQ=255)
                                                                             aataTTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCg                                                                      >  3:72567/1‑149 (MQ=255)
                                                                               tatTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGAt                                                                    >  5:89573/1‑149 (MQ=255)
                                                                                          tcAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCt                                                         >  8:58402/1‑149 (MQ=255)
                                                                                          tcAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCt                                                         >  5:92840/1‑149 (MQ=255)
                                                                                            aaGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCgcctgcc                                                                                      >  2:374142/1‑118 (MQ=255)
                                                                                            aaGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCgcctgcc                                                                                      >  2:368570/1‑118 (MQ=255)
                                                                                            aaGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCgcctgcc                                                                                      <  1:368570/118‑1 (MQ=255)
                                                                                            aaGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCgcctgcc                                                                                      <  1:374142/118‑1 (MQ=255)
                                                                                            aaGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTcc                                                          <  5:321564/146‑1 (MQ=255)
                                                                                            aaGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCtt                                                        >  2:280771/1‑148 (MQ=255)
                                                                                                  ggCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGctct                                                 >  8:225835/1‑149 (MQ=255)
                                                                                                  ggCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGc                                                    >  2:36700/1‑146 (MQ=255)
                                                                                                  ggCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGc                                                    <  1:36700/146‑1 (MQ=255)
                                                                                                   gcgcCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGctct                                                 <  2:246830/148‑1 (MQ=255)
                                                                                                     gcCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGctctctc                                              <  7:225835/149‑1 (MQ=255)
                                                                                                      ccTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGctctctct                                             <  4:226851/149‑1 (MQ=255)
                                                                                                       cTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAAcgcgc                                                                                                                  >  8:98453/1‑79 (MQ=255)
                                                                                                       cTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAAcgcgc                                                                                                                  <  7:98453/79‑1 (MQ=255)
                                                                                                           gTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTc                                                       >  6:150454/1‑134 (MQ=255)
                                                                                                           gTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTc                                                       <  5:150454/134‑1 (MQ=255)
                                                                                                            tCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAg                                       <  6:92840/149‑1 (MQ=255)
                                                                                                            tCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGAGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAg                                       >  5:407197/1‑149 (MQ=255)
                                                                                                                     ttgCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTAttt                               <  6:89573/148‑1 (MQ=255)
                                                                                                                     ttgCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCACCTGAGGGCTAttt                               <  1:280771/148‑1 (MQ=255)
                                                                                                                           tactacTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTccc                        >  6:419535/1‑149 (MQ=255)
                                                                                                                           tactacTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTccc                        >  5:240066/1‑149 (MQ=255)
                                                                                                                             ctactGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTcccc                       <  8:329833/148‑1 (MQ=255)
                                                                                                                             ctactGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTCCCCg                      <  6:407197/149‑1 (MQ=255)
                                                                                                                                             ttGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTgcgc                                                                                           >  7:36788/1‑64 (MQ=255)
                                                                                                                                             ttGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTgcgc                                                                                           <  8:36788/64‑1 (MQ=255)
                                                                                                                                               gCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTCCCCGTCACCGCCCCCTTGTc      <  7:76607/147‑1 (MQ=255)
                                                                                                                                                cGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTCCCCGTCACCGCCCCCTTGTccc    <  7:58402/148‑1 (MQ=255)
                                                                                                                                                 gAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTCCACCTCACCGACCCCTTGTCCCGa  >  6:173184/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TACGGAATGGGGTCAAACTGAATTTTCCTCAAAAAATTTCATATTTTTCATTGACTTACTATTGACATAATTTATATAATATTTTTGATCTCAAGGCTGGCGCCTGGGTCAAGGTTGTTGCTCTACTACTGGTTACCTTCCTTGCGAAGCAAGGGTATATTTGTATACAAAAGAAAACGCGCGCGCGAAAAAATTGCGATTGCGCCTGCCTTGAGTTCTCGGCCCGATTCTGCGCTCCTTCAGCTCTCTCTCCTGAGGGCTATTTTGCTCCCCGTCACCGCCCCCTTGTCCCGA  >  NC_002947/2215687‑2215980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑